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Rabbit Recombinant Monoclonal BCL6 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (AB243920), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (AB243920), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (AB243920), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (AB243920), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (AB243920), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Not recommended
Tested
Mouse
Predicted
Not recommended
Predicted
Rat
Predicted
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

Alternative names

BCL5, LAZ3, ZBTB27, ZNF51, BCL6, B-cell lymphoma 6 protein, BCL-6, B-cell lymphoma 5 protein, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51, BCL-5

Recommended products

Rabbit Recombinant Monoclonal BCL6 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
SP155
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab243920 is the carrier-free version of Anti-Bcl6 antibody [SP155] - C-terminal ab183308.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Bcl6 protein also known as B-cell lymphoma 6 is a transcriptional repressor important for the germinal center formation in B-cells. It has a molecular weight of approximately 95 kDa. Bcl6 is expressed mainly in B-cells T-cells and certain subsets of dendritic cells. It functions by binding to target DNA sequences where it represses the expression of specific genes leading to controlled cell proliferation differentiation and apoptosis. Researchers often study Bcl6 through the use of monoclonal antibodies particularly in immunohistochemistry (IHC).

Biological function summary

Bcl6 plays essential roles in immune response regulation and lymphocyte development. In germinal centers Bcl6 influences the process of somatic hypermutation and class switch recombination in B-cells which is a part of generating antibody diversity. It operates as a central player in a multiprotein complex that includes corepressors such as SMRT and NCoR which facilitate the repression of its target genes. This regulatory action helps maintain the precise balance of immune cell functions.

Pathways

Bcl6 integrates into the B-cell receptor (BCR) signaling and the JAK-STAT pathway. It interacts with molecules like STAT1 and STAT3 positively influencing the survival and proliferation of B-cells. In these pathways Bcl6's activity modulates the transcriptional landscape that determines cell fate decisions affecting cellular processes such as proliferation and survival in response to external cues.

Associated diseases and disorders

Bcl6 is relevant to B cell lymphomas specifically diffuse large B-cell lymphoma (DLBCL) where it is often overexpressed contributing to malignancy by preventing cell death. This pathology frequently involves chromosomal translocations that juxtapose the Bcl6 gene with immunoglobulin genes. The relationship between Bcl6 and other proteins like Bcl2 is noteworthy in this context as Bcl2 inhibits apoptosis further contributing to cancer progression. Mutations and dysregulated expression of Bcl6 have also shown links to autoimmune diseases where its role in cell differentiation and survival may lead to aberrant immune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Bcl6 with Anti-Bcl6 antibody [SP155] - C-terminal ab183308 at 1/100 dilution (1.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Bcl6 antibody [SP155] - C-terminal ab183308)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920)

    Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using Anti-Bcl6 antibody [SP155] - C-terminal ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Bcl6 antibody [SP155] - C-terminal ab183308).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920)

    Immunohistochemical analysis of paraffin-embedded Human follicular lymphoma tissue, labeling Bcl6 using Anti-Bcl6 antibody [SP155] - C-terminal ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Bcl6 antibody [SP155] - C-terminal ab183308).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920)

    Immunohistochemical analysis of paraffin-embedded Human HK lymphoma tissue, labeling Bcl6 using Anti-Bcl6 antibody [SP155] - C-terminal ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Bcl6 antibody [SP155] - C-terminal ab183308).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920)

    Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using Anti-Bcl6 antibody [SP155] - C-terminal ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Bcl6 antibody [SP155] - C-terminal ab183308).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920)

    Immunohistochemical analysis of paraffin-embedded Human B-cell lymphoma tissue, labeling Bcl6 using Anti-Bcl6 antibody [SP155] - C-terminal ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Bcl6 antibody [SP155] - C-terminal ab183308).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Bcl6 using Anti-Bcl6 antibody [SP155] - C-terminal ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Bcl6 antibody [SP155] - C-terminal ab183308).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [SP155] - BSA and Azide free (ab243920)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Bcl6 using Anti-Bcl6 antibody [SP155] - C-terminal ab183308 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Bcl6 antibody [SP155] - C-terminal ab183308).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com