Anti-BCL9 antibody

• WB

Project2518****

Western blot - Anti-BCL9 antibody (AB37305)

All lanes:

Western blot - Anti-BCL9 antibody (ab37305) at 2 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

Lane 2:

Jurkat nuclear extract lysate (<a href='/en-us/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate at 20 µg

Lane 4:

Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 149 kDa

Observed band size: 100 kDa,180 kDa,47 kDa

false

Exposure time: 20min

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-BCL9 antibody (AB37305)

ab37305 stained in HeLa cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37305 at 5μg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 μM for 1hour at room temperature.