Knockout Tested Rabbit Recombinant Monoclonal BCL9L antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Images
![Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (AB314441), expandable thumbnail](https://content.abcam.com/products/images/bcl9l-antibody-epr27491-35-bsa-and-azide-free-ab314441--western-blot-img421787.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (AB314441)")
![Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (AB314441), expandable thumbnail](https://content.abcam.com/products/images/bcl9l-antibody-epr27491-35-bsa-and-azide-free-ab314441--western-blot-img421785.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (AB314441)")
![Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (AB314441), expandable thumbnail](https://content.abcam.com/products/images/bcl9l-antibody-epr27491-35-bsa-and-azide-free-ab314441--western-blot-img421786.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (AB314441)")
![Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (AB314441), expandable thumbnail](https://content.abcam.com/products/images/bcl9l-antibody-epr27491-35-bsa-and-azide-free-ab314441--western-blot-img435375.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (AB314441)")
![Immunocytochemistry/ Immunofluorescence - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (AB314441), expandable thumbnail](https://content.abcam.com/products/images/bcl9l-antibody-epr27491-35-bsa-and-azide-free-ab314441--immunocytochemistry-immunofluorescence-img421888.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (AB314441)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1 (By similarity).
Alternative names
DLNB11, BCL9L, B-cell CLL/lymphoma 9-like protein, B-cell lymphoma 9-like protein, BCL9-like protein, Protein BCL9-2
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal BCL9L antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27491-35
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314441 is the carrier-free version of Anti-BCL9L antibody [EPR27491-35] ab314440.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441), expandable thumbnail](https://content.abcam.com/products/images/bcl9l-antibody-epr27491-35-bsa-and-azide-free-ab314441--western-blot-img421787.jpg "Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441)")
Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441)
BCL9L Western blot staining using rabbit Anti-BCL9L antibody
This data was developed using Anti-BCL9L antibody [EPR27491-35] ab314440, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: RKO (PMID: 24419084).
The identity of the bands between 75 kDa and 160 kDa are unknown.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.All lanes: Western blot - Anti-BCL9L antibody [EPR27491-35] (Anti-BCL9L antibody [EPR27491-35] ab314440) at 1/1000 dilution
Lane 1: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: RKO (human colon epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 190 kDa
Exposure time: 180s
![Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441), expandable thumbnail](https://content.abcam.com/products/images/bcl9l-antibody-epr27491-35-bsa-and-azide-free-ab314441--western-blot-img421785.jpg "Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441)")
Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441)
BCL9L Western blot staining using rabbit Anti-BCL9L antibody
This data was developed using Anti-BCL9L antibody [EPR27491-35] ab314440, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The identity of the band at 160 kDa is unknown.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.All lanes: Western blot - Anti-BCL9L antibody [EPR27491-35] (Anti-BCL9L antibody [EPR27491-35] ab314440) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: HeLa transfected with siRNA specifically targeti BCL9L whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 190 kDa
Exposure time: 180s
![Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441), expandable thumbnail](https://content.abcam.com/products/images/bcl9l-antibody-epr27491-35-bsa-and-azide-free-ab314441--western-blot-img421786.jpg "Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441)")
Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441)
BCL9L Western blot staining of MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate using rabbit Anti-BCL9L antibody
This data was developed using Anti-BCL9L antibody [EPR27491-35] ab314440, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The identity of the bands between 75 kDa and 160 kDa are unknown.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.All lanes: Western blot - Anti-BCL9L antibody [EPR27491-35] (Anti-BCL9L antibody [EPR27491-35] ab314440) at 1/1000 dilution
All lanes: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 190 kDa
Exposure time: 180s
![Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441), expandable thumbnail](https://content.abcam.com/products/images/bcl9l-antibody-epr27491-35-bsa-and-azide-free-ab314441--western-blot-img435375.jpg "Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441)")
Western blot - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441)
This data was developed using the same antibody clone in a different buffer formulation (abAB314440).
Western blot: Anti-BCL9L antibody [EPR27491-35] (Anti-BCL9L antibody [EPR27491-35] ab314440) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-BCL9L antibody [EPR27491-35] ab314440 was shown to bind specifically to BCL9L. A band was observed at 190 kDa in wild-type A549 cell lysates with no signal observed at this size in BCL9L knockout cell line. To generate this image, wild-type and BCL9L knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-BCL9L antibody [EPR27491-35] (Anti-BCL9L antibody [EPR27491-35] ab314440) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 40 µg
Lane 2: Wild-type A549 cell lysate at 20 µg
Lane 3: Wild-type A549 cell lysate at 10 µg
Lane 4: BCL9L knockout A549 cell lysate at 30 µg
Lane 5: HeLa cell lysate at 20 µg
Lane 6: HEK-293 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 190 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441), expandable thumbnail](https://content.abcam.com/products/images/bcl9l-antibody-epr27491-35-bsa-and-azide-free-ab314441--immunocytochemistry-immunofluorescence-img421888.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441)")
Immunocytochemistry/ Immunofluorescence - Anti-BCL9L antibody [EPR27491-35] - BSA and Azide free (ab314441)
This data was developed using Anti-BCL9L antibody [EPR27491-35] ab314440, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labelling BCL9L with Anti-BCL9L antibody [EPR27491-35] ab314440 at 1/100 (5.13 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1011 (2ug/ml) dilution (Green). Confocal image showing nuclear staining in SW480 cell line. Negative control: RKO (PMID: 24419084). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1011 (2ug/ml) dilution.
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