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Rabbit Recombinant Monoclonal BCMA antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

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Images

Flow Cytometry - Anti-BCMA antibody [EPR22457-260] (AB253242), expandable thumbnail
  • Immunoprecipitation - Anti-BCMA antibody [EPR22457-260] (AB253242), expandable thumbnail
  • Western blot - Anti-BCMA antibody [EPR22457-260] (AB253242), expandable thumbnail
  • Western blot - Anti-BCMA antibody [EPR22457-260] (AB253242), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPR22457-260] (AB253242), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/60
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Target data

Function

The protein expressed by the TNFRSF17 gene acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. It promotes B-cell survival and plays a role in regulating humoral immunity, while also activating NF-kappa-B and JNK. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

CD269, BCM, BCMA, TNFRSF17, Tumor necrosis factor receptor superfamily member 17, B-cell maturation protein

Recommended products

Rabbit Recombinant Monoclonal BCMA antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22457-260
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-BCMA antibody [EPR22457-260] (ab253242), expandable thumbnail

    Flow Cytometry - Anti-BCMA antibody [EPR22457-260] (ab253242)

    Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (left) or U266B1 (human multiple myeloma B lymphocyte cell line) (right) cells labeling BCMA with ab253242 at 1/60 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    Negative control: HEK-293T cells（PMID: 18025285.

  • Immunoprecipitation - Anti-BCMA antibody [EPR22457-260] (ab253242), expandable thumbnail

    Immunoprecipitation - Anti-BCMA antibody [EPR22457-260] (ab253242)

    BCMA was immunoprecipitated from 0.35 mg of U266B1 (human multiple myeloma B lymphocyte cell line) whole cell lysate with ab253242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253242 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: U266B1 whole cell lysate 10 μg (Input).
    Lane 2: ab253242 IP in U266B1 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab253242 in U266B1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-BCMA antibody [EPR22457-260] (ab253242)

    Predicted band size: 20 kDa

    Observed band size: 19 kDa, 23 kDa

  • Western blot - Anti-BCMA antibody [EPR22457-260] (ab253242), expandable thumbnail

    Western blot - Anti-BCMA antibody [EPR22457-260] (ab253242)

    Blocking and dilution buffer: 5% NFDM/TBST.

    BCMA is a glycoprotein.
    The molecular weight observed is consistent with what has been described in the literature (PMID: 23776238, 10908663,
    8527407)

    Negative control: HEK-293T (PMID: 18025285).

    All lanes: Western blot - Anti-BCMA antibody [EPR22457-260] (ab253242) at 1/1000 dilution

    Lane 1: U266B1 (human multiple myeloma B lymphocyte cell line) whole cell lysate at 20 µg

    Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa

    Observed band size: 19 kDa

    Exposure time: 3min

  • Western blot - Anti-BCMA antibody [EPR22457-260] (ab253242), expandable thumbnail

    Western blot - Anti-BCMA antibody [EPR22457-260] (ab253242)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-BCMA antibody [EPR22457-260] (ab253242) at 1/1000 dilution

    All lanes: Recombinant his-tagged human BCMA protein (ab219697, aa1-54) at 0.02 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPR22457-260] (ab253242), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPR22457-260] (ab253242)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U288B1 (human multiple myeloma B lymphocyte cell line) or HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling BCMA with ab253242 at 1/50 dilution, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
    Confocal image showing cytoplasmic and weak membranous staining in U266B1 cell line (PMID: 8527407) is observed.
    Negative control: HEK-293T cells（PMID: 18025285).
    The nuclear conter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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