Anti-BCMA antibody [EPR22457-260] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (AB254205)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U288B1 (human multiple myeloma B lymphocyte cell line) or HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling BCMA with ab253242 at 1/50 dilution, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic and weak membranous staining in U266B1 cell line (PMID : 8527407).
Negative control : HEK-293T cells (PMID : 18025285).
The nuclear conter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253242).

• Flow Cyt

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Flow Cytometry - Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (AB254205)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (left) or U266B1 (human multiple myeloma B lymphocyte cell line) (right) cells labeling BCMA with ab253242 at 1/60 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Negative control : HEK-293T cells (PMID : 18025285).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253242).

• IP

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Immunoprecipitation - Anti-BCMA antibody [EPR22457-260] - BSA and Azide free (AB254205)

BCMA was immunoprecipitated from 0.35 mg of U266B1 (human multiple myeloma B lymphocyte cell line) whole cell lysate with ab253242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253242 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

Lane 1 : U266B1 whole cell lysate 10 μg (Input).
Lane 2 : ab253242 IP in U266B1 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab253242 in U266B1 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253242).

All lanes:

Immunoprecipitation - Anti-BCMA antibody [EPR22457-260] (<a href='/en-us/products/primary-antibodies/bcma-antibody-epr22457-260-ab253242'>ab253242</a>)

Predicted band size: 20 kDa

Observed band size: 19 kDa,23 kDa

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