Anti-BCMA antibody [EPRBOB-R1-F1-24]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BCMA antibody [EPRBOB-R1-F1-24] (AB245940)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized U266B1 (human multiple myeloma B lymphocyte) cell line labeling BCMA with ab245940 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPRBOB-R1-F1-24] (AB245940)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U266B1 (human multiple myeloma B lymphocyte) cells labeling BCMA with ab245940 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in U266B1 cell line. Negative control cells : Jurkat (PMID : 11104810. The nuclear counterstain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

• IP

Unknown

Immunoprecipitation - Anti-BCMA antibody [EPRBOB-R1-F1-24] (AB245940)

BCMA was immunoprecipitated from 0.35 mg U266B1 (human multiple myeloma B lymphocyte) whole cell lysate with ab245940 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245940 at 1/300 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1 : U266B1 whole cell lysate 10 μg (Input).
Lane 2 : ab245940 IP in U266B1 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab245940 in U266B1 whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940)

Predicted band size: 20 kDa

Observed band size: 19 kDa,23 kDa

false

• WB

Unknown

Western blot - Anti-BCMA antibody [EPRBOB-R1-F1-24] (AB245940)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 23776238).

Negative control : Jurkat (PMID : 11104810).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-BCMA antibody [EPRBOB-R1-F1-24] (ab245940) at 1/300 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 2:

U266B1 (human multiple myeloma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 20 kDa

Observed band size: 19 kDa,23-30 kDa

false

Exposure time: 3min