Anti-BCOR antibody

• IP

Supplier Data

Immunoprecipitation - Anti-BCOR antibody (AB245423)

BCOR was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245423 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245423 at 1 μg/ml.

Lane 1 : ab245423 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-BCOR antibody (ab245423)

Predicted band size: 192 kDa

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• WB

Lab

Western blot - Anti-BCOR antibody (AB245423)

False colour image of Western blot : Anti-BCOR antibody staining at 1/1000 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab245423 was shown to bind specifically to BCOR. A band was observed at 192 kDa in wild-type HeLa cell lysates with no signal observed at this size in BCOR CRISPR-Cas9 edited cell line ab265125 (CRISPR-Cas9 edited cell lysate ab258327). The band observed in the CRISPR-Cas9 edited lysate lane below 192 kDa is likely to represent a truncated form of BCOR. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and BCOR CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-BCOR antibody (ab245423) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

BCOR CRISPR-Cas9 edited HeLa cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Observed band size: 192 kDa

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• WB

Supplier Data

Western blot - Anti-BCOR antibody (AB245423)

All lanes:

Western blot - Anti-BCOR antibody (ab245423) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 192 kDa

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Exposure time: 30s