Anti-BCOR antibody [EPR29210-51]

• WB

Supplier Data

Western blot - Anti-BCOR antibody [EPR29210-51] (AB323412)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 34333045

; PMID : 18795143).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting BCOR whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 238 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-BCOR antibody [EPR29210-51] (AB323412)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 34333045; PMID : 18795143).

In lanes 1-3, the lysates were stored at -80°C prior to Western Blotting.

To obtain stronger signals, the lysates in lanes 4-5 are freshly prepared and used immediately for Western blotting to minimize protein degradation.

The identity of the bands below 238 kDa in lanes 5-6 are unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Lane 3:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 40 µg

Lane 4:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 6:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 238 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-BCOR antibody [EPR29210-51] (AB323412)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : P815.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 34333045; PMID : 18795143).

To obtain stronger signals, the lysates are freshly prepared and used immediately for Western blotting to minimize protein degradation.

The identity of the bands below 238 kDa are unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412) at 1/1000 dilution

Lane 1:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Lane 2:

WEHI-231 (mouse B cell lymphoma B lymphocyte ) whole cell lysate at 20 µg

Lane 3:

A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 20 µg

Lane 4:

P815 (mouse mastocytoma mast Cell ) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 238 kDa,124 kDa

false

Exposure time: 180s