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Rabbit Recombinant Monoclonal BCOR antibody. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-BCOR antibody [EPR29210-51] (AB323412), expandable thumbnail
  • Western blot - Anti-BCOR antibody [EPR29210-51] (AB323412), expandable thumbnail
  • Western blot - Anti-BCOR antibody [EPR29210-51] (AB323412), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended
Mouse
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence-specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor. Involved in the repression of TFAP2A; impairs binding of BCL6 and KDM2B to TFAP2A promoter regions. Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem cells; the function implies inhibition of methylation on histone H3 'Lys-4' (H3K4me3) and 'Lys-36' (H3K36me2).

Additional Targets

Bcor

Alternative names

KIAA1575, BCOR, BCL-6 corepressor, BCoR

Rabbit Recombinant Monoclonal BCOR antibody. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29210-51
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412), expandable thumbnail

    Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: P815.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 34333045; PMID: 18795143).

    To obtain stronger signals, the lysates are freshly prepared and used immediately for Western blotting to minimize protein degradation.

    The identity of the bands below 238 kDa are unknown.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412) at 1/1000 dilution

    Lane 1: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

    Lane 2: WEHI-231 (mouse B cell lymphoma B lymphocyte ) whole cell lysate at 20 µg

    Lane 3: A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 20 µg

    Lane 4: P815 (mouse mastocytoma mast Cell ) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 238 kDa, 124 kDa

    Exposure time: 180s

  • Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412), expandable thumbnail

    Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 34333045; PMID: 18795143).

    In lanes 1-3, the lysates were stored at -80°C prior to Western Blotting.

    To obtain stronger signals, the lysates in lanes 4-5 are freshly prepared and used immediately for Western blotting to minimize protein degradation.

    The identity of the bands below 238 kDa in lanes 5-6 are unknown.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg

    Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

    Lane 3: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 40 µg

    Lane 4: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 6: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 238 kDa, 124 kDa

    Exposure time: 180s

  • Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412), expandable thumbnail

    Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 34333045

    ; PMID: 18795143).

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-BCOR antibody [EPR29210-51] (ab323412) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting BCOR whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 238 kDa, 124 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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