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AB256126

Anti-Bcr-abl antibody [7C6] - BSA and Azide free

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Mouse Recombinant Monoclonal BCR antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

BCR1, D22S11, BCR, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Bcr-abl antibody [7C6] - BSA and Azide free (AB256126)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bcr-abl antibody [7C6] - BSA and Azide free (AB256126)

This data was developed using ab187831, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 fixed K562 (human chronic myelogenous leukemia lymphoblast) cells labeling Bcr-abl with ab187831 at 1/250 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Confocal image showing strongly cytoplasmic staining in K-562 cell line. The nuclear counter stain id DAPI (Blue). Tubulin is detected using ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red).

The negative controls are as follows :
-ve control 1 : ab187831 at 1/250 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) secondary at 1/500 dilution.
-ve control 2 : Primary (ab179513) at 1/200 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1/1000 dilution.

Western blot - Anti-Bcr-abl antibody [7C6] - BSA and Azide free (AB256126)
  • WB

Lab

Western blot - Anti-Bcr-abl antibody [7C6] - BSA and Azide free (AB256126)

This data was developed using ab187831, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Lysates should be non-boiled as boiling may cause protein aggregates.

Exposure time : 59 seconds

All lanes:

Western blot - Anti-Bcr-abl antibody [7C6] - BSA and Azide free (ab256126) at 1/1000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 197 kDa

Observed band size: 142 kDa,210 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7C6

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes an epitope within the amino acid sequence SSINEEITPRRQS of Bcr/Abl.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab256126 is the carrier-free version of ab187831.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bcr-abl fusion protein also known as BCR-ABL or BCR/c-ABL results from a genetic abnormality where parts of the BCR and ABL genes combine together. This fusion occurs on chromosome 22 known as the Philadelphia chromosome leading to a well-known oncogenic protein with a molecular mass of approximately 210 kDa. The Bcr-abl is primarily expressed in hematopoietic cells and plays a critical role in cellular proliferation and survival. The protein's kinase domain derived from c-ABL exhibits tyrosine kinase activity regulating downstream signaling pathways involved in cellular growth and division.
Biological function summary

Bcr-abl significantly alters normal cell functions by constantly activating signals that drive cell cycle progression and inhibit apoptosis. This protein forms a complex with various signaling molecules enhancing proliferative signals within the cell. The presence of BCR/ABL leads to abnormal interaction with cell cycle regulators and contributes to dysregulated growth characteristic of cancerous cells. Through these interactions Bcr-abl affects DNA repair processes and cytoskeletal remodelling both of which are critical for maintaining genomic stability and cellular integrity.

Pathways

Bcr-abl influences multiple signaling cascades prominently including the PI3K-AKT and RAS-RAF-MEK-ERK pathways. These pathways are pivotal for transmitting growth factor signals that promote cell survival and proliferation. BCR-ABL activates these pathways independently of growth factor binding which leads to uncontrolled proliferation and resistance to apoptosis. Additionally Bcr-abl interacts with proteins such as GRB2 and SHC which are parts of these signaling networks further amplifying growth and survival signals in affected cells.

Bcr-abl is strongly associated with chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL). These malignancies arise due to the continuous activity of the aberrant Bcr-abl protein which leads to the overproduction of malignant leukocytes. The disease progression in CML illustrates a link between the Bcr-abl expression and the transformation of normal hematopoietic stem cells into leukemic cells. Furthermore this protein relates to the development of leukemias by interacting with downstream effectors like STAT5 which aids in the transcription of anti-apoptotic genes further contributing to cancer cell survival and proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form (PubMed : 17116687, PubMed : 1903516, PubMed : 7479768). The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (PubMed : 23940119, PubMed : 7479768). The amino terminus contains an intrinsic kinase activity (PubMed : 1657398). Functions as an important negative regulator of neuronal RAC1 activity (By similarity). Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity (PubMed : 17116687). Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation (PubMed : 23940119).
See full target information BCR
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Product promise

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For full details, please see our Terms & Conditions

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