AB233709

Anti-Bcr antibody [1E11G12]

Name: Anti-Bcr antibody [1E11G12] (ab233709) | Abcam
Brand: Abcam Limited
SKU: AB233709
Price: 495 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal BCR antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BCR aa 100-300.

View Alternative Names

BCR1, D22S11, BCR, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26

4 Images

Flow Cytometry - Anti-Bcr antibody [1E11G12] (AB233709)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bcr antibody [1E11G12] (AB233709)

Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Bcr with ab233709 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-Bcr antibody [1E11G12] (AB233709)

All lanes:

Western blot - Anti-Bcr antibody [1E11G12] (ab233709) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 3:

Ramos (human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 143 kDa

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Supplier Data

Western blot - Anti-Bcr antibody [1E11G12] (AB233709)

All lanes:

Western blot - Anti-Bcr antibody [1E11G12] (ab233709) at 1/500 dilution

All lanes:

Recombinant human Bcr (aa139-28) protein

Predicted band size: 143 kDa

true

Supplier Data

Western blot - Anti-Bcr antibody [1E11G12] (AB233709)

All lanes:

Western blot - Anti-Bcr antibody [1E11G12] (ab233709) at 1/500 dilution

Lane 1:

Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

Human Bcr (aa139-280)-IgG-Fc-transfected HEK-293 cell lysate

Predicted band size: 143 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1E11G12

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human BCR aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

P11274

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bcr protein also known as breakpoint cluster region protein plays an important role in cellular signaling. It has a molecular mass of approximately 160 kDa and is expressed in many cell types such as hematopoietic cells. Bcr functions as a GTPase-activating protein (GAP) for p21rac and other small GTP-binding proteins. This activity influences cellular processes such as growth and differentiation. Bcr is also known for its involvement in the formation of the Bcr-Abl fusion protein an important factor in certain leukemias.

Biological function summary

The Bcr protein acts within cells to regulate signal transduction and cytoskeletal organization. It is part of the pre-B cell receptor (pre-BCR) signaling complex influencing B-cell development. Its GAP activity is important for the regulation of small GTPases impacting cell migration and adhesion. Alterations in Bcr function can affect cellular responses such as apoptosis making it a focus for research into targeted therapies for hematological conditions.

Pathways

The Bcr protein is an important player in signaling pathways involving the Ras superfamily of small GTPases. It is notably involved in the Rho GTPase pathway tied to cytoskeletal dynamics and cell cycle progression. Bcr's interaction with these pathways puts it in relation to proteins like Rac and Cdc42 which are vital for various cellular processes. Disruption in Bcr-associated pathways can lead to aberrant cell growth and metastasis.

Bcr has a well-documented link to certain leukemias particularly chronic myeloid leukemia (CML). The Bcr-Abl fusion protein resulting from the translocation of chromosomes 9 and 22 is central to CML pathology. Additionally Bcr connects with other proteins such as Abl in this disease context. Further disruptions in Bcr signaling can relate to disorders involving immune system dysregulation demonstrating the protein's relevance in both oncology and immunology research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form (PubMed : 17116687, PubMed : 1903516, PubMed : 7479768). The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (PubMed : 23940119, PubMed : 7479768). The amino terminus contains an intrinsic kinase activity (PubMed : 1657398). Functions as an important negative regulator of neuronal RAC1 activity (By similarity). Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity (PubMed : 17116687). Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation (PubMed : 23940119).

See full target information BCR

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 19: PubMed29966365

2018

NR1 and NR3B Composed Intranuclear -methyl-d-aspartate Receptor Complexes in Human Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tibor Hajdú,Tamás Juhász,Csilla Szűcs-Somogyi,Kálmán Rácz,Róza Zákány

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bcr antibody [1E11G12]

Flow Cytometry - Anti-Bcr antibody [1E11G12] (AB233709)

Western blot - Anti-Bcr antibody [1E11G12] (AB233709)

Western blot - Anti-Bcr antibody [1E11G12] (AB233709)

Western blot - Anti-Bcr antibody [1E11G12] (AB233709)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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