AB245511

Anti-Bcr antibody

Name: Anti-Bcr antibody (ab245511) | Abcam
Brand: Abcam Limited
SKU: AB245511

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(1 Publication)

Rabbit Polyclonal BCR antibody. Suitable for IP, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BCR aa 50-100.

View Alternative Names

BCR1, D22S11, BCR, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Bcr antibody (AB245511)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bcr antibody (AB245511)

Formalin-fixed, paraffin-embedded K562 (human chronic myelogenous leukemia cell line from bone marrow, contains the chromosomal translocation, t(9 : 22) that creates the BCR/ABL fusion gene) cells stained for Bcr (Green) using ab245511 at 1/1,000 dilution. Green-fluorescent goat anti rabbit IgG - Alexa Fluor^® 488 was used as the secondary antibody at a dilution of 1/1,000.

Flow Cytometry (Intracellular) - Anti-Bcr antibody (AB245511)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Bcr antibody (AB245511)

Flow cytometric analysis of 1 x 10⁶ K562 ( human chronic myelogenous leukemia cell line from bone marrow) cells stained with 0.25 μg ab245511 in a 150 μl reaction (Blue). Isotype anti-KLH control (black), no antibody (red).

Supplier Data

Immunoprecipitation - Anti-Bcr antibody (AB245511)

Bcr was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded). Western blot was performed on the immunoprecipitate using a different rabbit anti-Bcr primary antibody.

Lane 1 : ab245511 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Bcr antibody (ab245511)

Predicted band size: 143 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), IP

applications

Immunogen

Synthetic Peptide within Human BCR aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P11274

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10 µg/mg of lysate", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/2000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.25 µg/cells for 6 µg/cells", "FlowCytIntra-species-notes": "(in 150 μL volume)" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245511 was affinity purified using an epitope specific to Bcr immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bcr protein also known as breakpoint cluster region protein plays an important role in cellular signaling. It has a molecular mass of approximately 160 kDa and is expressed in many cell types such as hematopoietic cells. Bcr functions as a GTPase-activating protein (GAP) for p21rac and other small GTP-binding proteins. This activity influences cellular processes such as growth and differentiation. Bcr is also known for its involvement in the formation of the Bcr-Abl fusion protein an important factor in certain leukemias.

Biological function summary

The Bcr protein acts within cells to regulate signal transduction and cytoskeletal organization. It is part of the pre-B cell receptor (pre-BCR) signaling complex influencing B-cell development. Its GAP activity is important for the regulation of small GTPases impacting cell migration and adhesion. Alterations in Bcr function can affect cellular responses such as apoptosis making it a focus for research into targeted therapies for hematological conditions.

Pathways

The Bcr protein is an important player in signaling pathways involving the Ras superfamily of small GTPases. It is notably involved in the Rho GTPase pathway tied to cytoskeletal dynamics and cell cycle progression. Bcr's interaction with these pathways puts it in relation to proteins like Rac and Cdc42 which are vital for various cellular processes. Disruption in Bcr-associated pathways can lead to aberrant cell growth and metastasis.

Bcr has a well-documented link to certain leukemias particularly chronic myeloid leukemia (CML). The Bcr-Abl fusion protein resulting from the translocation of chromosomes 9 and 22 is central to CML pathology. Additionally Bcr connects with other proteins such as Abl in this disease context. Further disruptions in Bcr signaling can relate to disorders involving immune system dysregulation demonstrating the protein's relevance in both oncology and immunology research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form (PubMed : 17116687, PubMed : 1903516, PubMed : 7479768). The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (PubMed : 23940119, PubMed : 7479768). The amino terminus contains an intrinsic kinase activity (PubMed : 1657398). Functions as an important negative regulator of neuronal RAC1 activity (By similarity). Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity (PubMed : 17116687). Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation (PubMed : 23940119).

See full target information BCR

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

IUBMB life 75:659-672 PubMed36961205

2023

FOXC2-induced circCASK aggravates colorectal cancer progression by upregulating SIX1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei Zou,Yong Huang,Yuan Chen,Zhaoying Wu,Hao Xie,Hailang Zhou,Chungen Xing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bcr antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bcr antibody (AB245511)

Flow Cytometry (Intracellular) - Anti-Bcr antibody (AB245511)

Immunoprecipitation - Anti-Bcr antibody (AB245511)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise