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AB40779

Anti-Bcr antibody [EP535Y]

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(1 Publication)

Rabbit Recombinant Monoclonal BCR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BCR1, D22S11, BCR, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcr antibody [EP535Y] (AB40779)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcr antibody [EP535Y] (AB40779)

ab40779, staining human lymphoma by immunohistochemistry, paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Bcr antibody [EP535Y] (AB40779)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bcr antibody [EP535Y] (AB40779)

ab40779, staining HeLa cells by immunofluorescence.

Western blot - Anti-Bcr antibody [EP535Y] (AB40779)
  • WB

Lab

Western blot - Anti-Bcr antibody [EP535Y] (AB40779)

All lanes:

Western blot - Anti-Bcr antibody [EP535Y] (ab40779) at 1/1000 dilution

All lanes:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 143 kDa

Observed band size: 150 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP535Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% EDTA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bcr protein also known as breakpoint cluster region protein plays an important role in cellular signaling. It has a molecular mass of approximately 160 kDa and is expressed in many cell types such as hematopoietic cells. Bcr functions as a GTPase-activating protein (GAP) for p21rac and other small GTP-binding proteins. This activity influences cellular processes such as growth and differentiation. Bcr is also known for its involvement in the formation of the Bcr-Abl fusion protein an important factor in certain leukemias.
Biological function summary

The Bcr protein acts within cells to regulate signal transduction and cytoskeletal organization. It is part of the pre-B cell receptor (pre-BCR) signaling complex influencing B-cell development. Its GAP activity is important for the regulation of small GTPases impacting cell migration and adhesion. Alterations in Bcr function can affect cellular responses such as apoptosis making it a focus for research into targeted therapies for hematological conditions.

Pathways

The Bcr protein is an important player in signaling pathways involving the Ras superfamily of small GTPases. It is notably involved in the Rho GTPase pathway tied to cytoskeletal dynamics and cell cycle progression. Bcr's interaction with these pathways puts it in relation to proteins like Rac and Cdc42 which are vital for various cellular processes. Disruption in Bcr-associated pathways can lead to aberrant cell growth and metastasis.

Bcr has a well-documented link to certain leukemias particularly chronic myeloid leukemia (CML). The Bcr-Abl fusion protein resulting from the translocation of chromosomes 9 and 22 is central to CML pathology. Additionally Bcr connects with other proteins such as Abl in this disease context. Further disruptions in Bcr signaling can relate to disorders involving immune system dysregulation demonstrating the protein's relevance in both oncology and immunology research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form (PubMed : 17116687, PubMed : 1903516, PubMed : 7479768). The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (PubMed : 23940119, PubMed : 7479768). The amino terminus contains an intrinsic kinase activity (PubMed : 1657398). Functions as an important negative regulator of neuronal RAC1 activity (By similarity). Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity (PubMed : 17116687). Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation (PubMed : 23940119).
See full target information BCR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of proteome research 11:4847-62 PubMed22889411

2012

Proteomics strategy to identify substrates of LNX, a PDZ domain-containing E3 ubiquitin ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengguang Guo,Eli Song,Sucan Ma,Xiaorong Wang,Shijuan Gao,Chen Shao,Siqi Hu,Lulu Jia,Rui Tian,Tao Xu,Youhe Gao
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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