Anti-BCRP/ABCG2 antibody [EPR20080]
- RabMAb
- Recombinant
- KO Validated
- 20ul selling size
- What is this?
4
(6 Reviews)
|
(56 Publications)
Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) is a rabbit monoclonal antibody detecting BCRP/ABCG2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
View Alternative Names
CD338, ABCP, BCRP, BCRP1, MXR, ABCG2, Broad substrate specificity ATP-binding cassette transporter ABCG2, ATP-binding cassette sub-family G member 2, Breast cancer resistance protein, CDw338, Mitoxantrone resistance-associated protein, Placenta-specific ATP-binding cassette transporter, Urate exporter
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed A549 (human lung carcinoma cell line) cell line labeling BCRP/ABCG2 with ab207732 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)
Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on capillaries of human glioma (PMID : 20216549). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on human placenta (PMID : 12237881). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of human liver (PMID : 12237881). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of mouse liver (PMID : 12237881). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling BCRP/ABCG2 with ab207732 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on biliary canaliculis of rat liver (PMID : 12237881). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)
Blocking/Dilution buffer : 5% NFDM/TBST.
For Lysate preparation protocol, please refer to the protocol section of the website and/or here.
All lanes:
Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution
Lane 1:
Human placenta, the lysate was prepared in RIPA lysate buffer and boiled before loading at 10 µg
Lane 2:
Human placenta, the lysate was prepared in RIPA lysate buffer and was not boiled before loading at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 72 kDa
Observed band size: 75 kDa
true
Exposure time: 15s
- WB
Lab
Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)
Lanes 1 - 4 : Merged signal (red and green). Green - ab207732 observed at 70 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab207732 was shown to react with BCRP/ABCG2 in wild-type A549 cells in Western blot with loss of signal observed in ABCG2 knockout cell line ab259773 (ABCG2 knockout cell lysate ab259778). Wild-type A549 and ABCG2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab207732 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
ABCG2 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human ABCG2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-abcg2-knockout-a549-cell-line-ab259773'>ab259773</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
THP-1 cell lysate at 20 µg
Predicted band size: 72 kDa
Observed band size: 70 kDa
false
- WB
Supplier Data
Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)
Blocking/Dilution buffer : 5% NFDM/TBST.
For Lysate preparation protocol, please refer to the protocol section of the website and/or here.
All lanes:
Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution
Lane 1:
A549 (human lung carcinoma cell line) whole cell lysate, the lysate was prepared in RIPA lysate buffer and boiled before loading at 20 µg
Lane 2:
A549 (human lung carcinoma cell line) whole cell lysate, the lysate was prepared in RIPA lysate buffer and was not boiled before loading at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 72 kDa
Observed band size: 75 kDa
true
Exposure time: 3s
- WB
Supplier Data
Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (AB207732)
Blocking/Dilution buffer : 5% NFDM/TBST.
For Lysate preparation protocol, please refer to the protocol section of the website and/or here.
Negative control : Huh7 (PMID : 26327240, PMID : 18820285).
All lanes:
Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) at 1/1000 dilution
Lane 1:
HepG2 (human liver hepatocellular carcinoma cell line), whole cell lysate, the lysate was prepared in 1% SDS Hot Lysate buffer and boiled before loading at 20 µg
Lane 2:
Huh7 (human liver hepatocellular carcinoma epithelial cell line), whole cell lysate, the lysate was prepared in 1% SDS Hot Lysate buffer and boiled before loading at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 72 kDa
Observed band size: 75 kDa
true
Exposure time: 1min
Related conjugates and formulations (1)
-
Anti-BCRP/ABCG2 antibody [EPR20080] - BSA and Azide free
Reactivity data
Product details
What is this antibody validated in?
Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of BCRP/ABCG2?
Anti-BCRP/ABCG2 [EPR20080] (ab207732) specifically detects a band for BCRP/ABCG2 (UniProt: Q9UNQ0) at a molecular weight of 72kDa.
Trusted by the scientific community
Anti-BCRP/ABCG2 [EPR20080] (ab207732) was first used in a scientific publication in 2017 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-BCRP/ABCG2 [EPR20080] (ab207732) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-BCRP/ABCG2 antibody [EPR20080] (ab207732) has been confirmed by Western blot testing in ABCG2 Knockout A549 cell line, ab259773.
Other related products
We have a range of other formats of antibody clone [EPR20080] also available for your convenience: ab207732, Carrier free - ab232517
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (56)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in oncology 15:1558184 PubMed40548120
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 26: PubMed40362377
2025
Applications
Unspecified application
Species
Unspecified reactive species
Pharmacology research & perspectives 13:e70111 PubMed40325522
2025
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 15:8735 PubMed40082588
2025
Applications
Unspecified application
Species
Unspecified reactive species
British journal of pharmacology 182:2049-2066 PubMed39909477
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of biological sciences 21:382-399 PubMed39744423
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular medicine 54: PubMed38994756
2024
Applications
Unspecified application
Species
Unspecified reactive species
Redox biology 75:103262 PubMed38981367
2024
Applications
Unspecified application
Species
Unspecified reactive species
Biology direct 19:49 PubMed38910243
2024
Applications
Unspecified application
Species
Unspecified reactive species
Communications biology 7:177 PubMed38418614
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com