AB205067

Anti-BDNF antibody [3B2]

Name: Anti-BDNF antibody [3B2] Mouse monoclonal (ab205067) | Abcam
Brand: Abcam Limited
SKU: AB205067
Price: 475 USD
Availability: InStock

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(46 Publications )

Mouse Monoclonal BDNF antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Chinese hamster, Mouse, Rat samples. Cited in 46 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BDNF.

View Alternative Names

Neurotrophic factor BDNF precursor form, proBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [3B2] (AB205067)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [3B2] (AB205067)

Immunofluorescent analysis of U2OS cells labeling BDNF with ab205067 at 0.33 μg/ml, followed by Goat anti-mouse AlexaFluor488 secondary antibody. For nuclear staining DAPI was used. A : proBDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [3B2] (AB205067)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [3B2] (AB205067)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebral cortex tissue sections from Alzheimer's disease patients labeling BDNF with ab205067 at 5 μg/ml, followed by peroxidase secondary detection and DAB staining. A : BDNF staining by ab205067; B.Negative staining without primary antibody. Sections were counterstained with toluidine blue.

Supplier Data

Western blot - Anti-BDNF antibody [3B2] (AB205067)

All lanes:

Western blot - Anti-BDNF antibody [3B2] (ab205067) at 5 µg/mL

All lanes:

10 µl of CHO cell culture supernatant containing BDNF at 10 µL

Secondary

All lanes:

HRP-conjugated Goat anti-Mouse IgG

Predicted band size: 27 kDa

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Lab

Western blot - Anti-BDNF antibody [3B2] (AB205067)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel ran at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for 1 hour before incubation with ab205067 (2.5 microgram /mL) overnight at 4°C. Antibody binding was detected using infrared labelled goat anti-mouse IgG (green) at a 1 : 10,000 dilution for 1 hour at room temperature.

All lanes:

Western blot - Anti-BDNF antibody [3B2] (ab205067) at 2.5 µg/mL

Lane 1:

Human hippocampus

Lane 2:

Rat hippocampus

Lane 3:

Mouse hippocampus

Secondary

All lanes:

Goat anti-Mouse800 Licor at 1/10000 dilution

Predicted band size: 27 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3B2

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human, Chinese hamster

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human BDNF.

P23560

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.33-20 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed : 11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.. Neurotrophic factor BDNF precursor form. Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed : 24908487, PubMed : 29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).

See full target information BDNF

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Publications (46)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in psychiatry 16:1586613 PubMed40534913

2025

Correlation study of BDNF/TrkB/CREB, violence, and cognitive function in first-episode drug-naive schizophrenia patients.

Applications

Unspecified application

Species

Unspecified reactive species

Tiankai Jiang,Zhipeng Li,Tao Yu,Xudong Zhou,Tiantian Jiang,Yuhang Liang,Chen Yu,Min Zhu,Wenyu Wu

International journal of molecular sciences 26: PubMed40003962

2025

Non-Invasive Assessment of Neurogenesis Dysfunction in Fetuses with Early-Onset Growth Restriction Using Fetal Neuronal Exosomes Isolating from Maternal Blood: A Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

Vladislava Gusar,Natalia Kan,Anastasia Leonova,Vitaliy Chagovets,Victor Tyutyunnik,Zarine Khachatryan,Ekaterina Yarotskaya,Gennadiy Sukhikh

Journal of neuroinflammation 22:6 PubMed39800730

2025

TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Wang,Diqing Ouyang,Lin Li,Yunchuan Cao,Yingwen Wang,Nina Gu,Zhaosi Zhang,Zhao Li,Shuang Tang,Hui Tang,Yuan Zhang,Xiaochuan Sun,Jin Yan

CNS neuroscience & therapeutics 30:e14750 PubMed38898731

2024

Cognitive decline in Sprague-Dawley rats induced by neuroplasticity changes after occlusal support loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Wang,Qian Pang,Jiangqi Hu,Bin Luo,Yunping Lu,Xu Sun,Shixiang Meng,Qingsong Jiang

PloS one 19:e0300888 PubMed38512830

2024

Trolox and recombinant Irisin as a potential strategy to prevent neuronal damage induced by random positioning machine exposure in differentiated HT22 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Bonanni,Ida Cariati,Anna Maria Rinaldi,Mario Marini,Giovanna D'Arcangelo,Umberto Tarantino,Virginia Tancredi

Phytotherapy research : PTR 38:2619-2640 PubMed38488455

2024

Salidroside directly activates HSC70, revealing a new role for HSC70 in BDNF signalling and neurogenesis after cerebral ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Wenfang Lai,Rui Luo,Yuheng Tang,Zhengshuang Yu,Binbin Zhou,Zelin Yang,John Brown,Guizhu Hong

Genes to cells : devoted to molecular & cellular mechanisms 29:432-437 PubMed38467515

2024

Long-term sorbitol consumption affects the hippocampus and alters cognitive function in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haruna Yokoi,Jingshu Wang,Yoriko Ikuyo,Mitsuyoshi Yamada,Yosuke Shikama,Masae Furukawa,Kenji Matsushita

International journal of ophthalmology 17:34-41 PubMed38239953

2024

Effect of Sonic hedgehog gene-modified bone marrow mesenchymal stem cells on graft-induced retinal gliosis and retinal ganglion cells survival in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Wang,Hai-Chun Li,Jin Ma,Xi-Ling Yu

European journal of histochemistry : EJH 67: PubMed37491974

2023

Dexmedetomidine attenuates neuroinflammation and microglia activation in LPS-stimulated BV2 microglia cells through targeting circ-Shank3/miR-140-3p/TLR4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guangbao He,Yibo He,Hongwei Ni,Kai Wang,Yijun Zhu,Yang Bao

Journal of neuroinflammation 20:129 PubMed37245024

2023

The neuroprotective N-terminal amyloid-β core hexapeptide reverses reactive gliosis and gliotoxicity in Alzheimer's disease pathology models.

Applications

Unspecified application

Species

Unspecified reactive species

Megan J Lantz,Alyssa M Roberts,Donovan D Delgado,Robert A Nichols

View all publications

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