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AB223354

Anti-BDNF antibody

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(3 Publications)

Rabbit Polyclonal BDNF antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human BDNF.

View Alternative Names

Neurotrophic factor BDNF precursor form, proBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (AB223354)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (AB223354)

4% formaldehyde-fixed SK-N-BE cells stained for BDNF (green) using ab223354 at 1/100 dilution in ICC/IF. Counterstain : Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min RT, 5 min RT.

(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223354 (D) Merge.

Western blot - Anti-BDNF antibody (AB223354)
  • WB

Supplier Data

Western blot - Anti-BDNF antibody (AB223354)

Blocking buffer : 5% milk in TBST.

Color Development : TMB solution.

Primary and secondary antibodies were incubated for 1 hour at RT.

All lanes:

Western blot - Anti-BDNF antibody (ab223354) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP at 1/2000 dilution

Predicted band size: 27 kDa

Observed band size: 27.9 kDa

false

Exposure time: 12min

Western blot - Anti-BDNF antibody (AB223354)
  • WB

Supplier Data

Western blot - Anti-BDNF antibody (AB223354)

Blocking buffer : 5% milk in TBST.

Color Development : TMB solution.

Primary and secondary antibodies were incubated for 1 hour at RT.

All lanes:

Western blot - Anti-BDNF antibody (ab223354) at 1/1000 dilution

All lanes:

Mouse brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP at 1/2000 dilution

Predicted band size: 27 kDa

Observed band size: 27.9 kDa

false

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human BDNF. The exact immunogen used to generate this antibody is proprietary information.

P23560

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the BDNF gene is a critical signaling molecule that activates pathways downstream of NTRK2, as well as the heterodimeric receptor formed by NGFR and SORCS2. During development, it aids the survival and differentiation of specific neuronal populations in both the peripheral and central nervous systems. It is involved in axonal growth, pathfinding, and modulation of dendritic growth and morphology. In adult synapses, BDNF is a key regulator of synaptic transmission and plasticity in various CNS regions, contributing to processes like long-term potentiation, long-term depression, some short-term synaptic plasticity forms, and the homeostatic regulation of intrinsic neuronal excitability. Signaling through NGFR and SORCS2 is linked to synaptic plasticity and long-term depression, while its binding to these receptors also promotes neuronal apoptosis. This supplementary information is collated from multiple sources and compiled automatically.
See full target information BDNF
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 13: PubMed38397748

2024

Oxidative Stress Plays an Important Role in Glutamatergic Excitotoxicity-Induced Cochlear Synaptopathy: Implication for Therapeutic Molecules Screening.

Applications

Unspecified application

Species

Unspecified reactive species

Anissa Rym Saidia,Florence François,François Casas,Ilana Mechaly,Stéphanie Venteo,Joseph T Veechi,Jérôme Ruel,Jean-Luc Puel,Jing Wang

American journal of physiology. Renal physiology 319:F930-F940 PubMed32865014

2020

(Pro)renin receptor decoy peptide PRO20 protects against adriamycin-induced nephropathy by targeting the intrarenal renin-angiotensin system.

Applications

Unspecified application

Species

Unspecified reactive species

Renfei Luo,Kevin Yang,Fei Wang,Chuanming Xu,Tianxin Yang

Molecular therapy : the journal of the American Society of Gene Therapy 28:503-522 PubMed31843449

2019

Increased Microglial Exosomal miR-124-3p Alleviates Neurodegeneration and Improves Cognitive Outcome after rmTBI.

Applications

Unspecified application

Species

Unspecified reactive species

Xintong Ge,Mengtian Guo,Tianpeng Hu,Wenzhu Li,Shan Huang,Zhenyu Yin,Ying Li,Fanglian Chen,Luoyun Zhu,Chunsheng Kang,Rongcai Jiang,Ping Lei,Jianning Zhang
View all publications
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