Rabbit Polyclonal BDNF antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human BDNF.
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- Folia neuropathologica 61:273-2902023Astragaloside IV inhibits experimental autoimmune encephalomyelitis by modulating the polarization of both microglia/macrophages and astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingwen Yu,Bingtao Mu,Minfang Guo,Chunyun Liu,Tao Meng,Yuqing Yan,Lijuan Song,Jiezhong Yu,Gajendra Kumar,Cungen MaPubMed 37818688
- Asian journal of pharmaceutical sciences 18:1007832023Selective ischemic-hemisphere targeting Ginkgolide B liposomes with improved solubility and therapeutic efficacy for cerebral ischemia-reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Li,Miaomiao Zhang,Shiyi Li,Longlong Zhang,Jisu Kim,Qiujun Qiu,Weigen Lu,Jianxin WangPubMed 36891470
- International journal of molecular sciences 23:2022Improvement of Cognitive Function in Ovariectomized Rats by Human Neural Stem Cells Overexpressing Choline Acetyltransferase via Secretion of NGF and BDNF.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Jung Yoon,Yunseo Choi,Dongsun ParkPubMed 35628371
- Antioxidants (Basel, Switzerland) 11:2022Baicalein Exerts Therapeutic Effects against Endotoxin-Induced Depression-like Behavior in Mice by Decreasing Inflammatory Cytokines and Increasing Brain-Derived Neurotrophic Factor Levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHsin-Tzu Liu,Yu-Ning Lin,Ming-Cheng Tsai,Ya-Chi Wu,Ming-Chung LeePubMed 35624812
- Molecules (Basel, Switzerland) 27:2022Dioscin-Mediated Autophagy Alleviates MPP-Induced Neuronal Degeneration: An In Vitro Parkinson's Disease Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShofiul Azam,Md Ezazul Haque,Duk-Yeon Cho,Joon-Soo Kim,Md Jakaria,In-Su Kim,Dong-Kug ChoiPubMed 35566180
- BMC psychiatry 22:1822022BDNF-TrkB signaling-mediated upregulation of Narp is involved in the antidepressant-like effects of (2R,6R)-hydroxynorketamine in a chronic restraint stress mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingsha Ju,Jiaojiao Yang,Tingting Zhu,Panmiao Liu,Jianjun YangPubMed 35291971
- International journal of molecular sciences 22:2021Analysis of Antidepressant-like Effects and Action Mechanisms of GSB-106, a Small Molecule, Affecting the TrkB Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulia V Vakhitova,Tatiana S Kalinina,Liana F Zainullina,Anastasiya Yu Lusta,Anna V Volkova,Nikita V Kudryashov,Tatiana A Gudasheva,Alexander A Shimshirt,Ilya A Kadnikov,Mikhail V Voronin,Sergei B SeredeninPubMed 34948177
- Frontiers in cellular neuroscience 15:7720472021General Anesthesia During Neurodevelopment Reduces Autistic Behavior in Adult BTBR Mice, a Murine Model of Autism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianchen Cui,Jiho Park,Xianshu Ju,Yulim Lee,Boohwi Hong,Jeonghoon Ahn,Yoon Hee Kim,Youngkwon Ko,Seok-Hwa Yoon,Chaeseong Lim,Sun Yeul Lee,Sung-Oh Huh,Jun Young Heo,Woosuk ChungPubMed 34912193
- Experimental and therapeutic medicine 22:14422021Repeated exposure to sevoflurane in neonatal rats impairs cognition in adulthood via the PKA-CREB-BDNF signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJili Zhao,Jinyu Ren,Shuang Liu,Yanan Gong,Ping Meng,Haitao Tan,Yonggang ChenPubMed 34721684
- Brain and behavior 11:e23002021Genistein acts as antidepressant agent against chronic mild stress-induced depression model of rats through augmentation of brain-derived neurotrophic factor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingxiu Chang,Li Zhang,Huiyu Dai,Ling SunPubMed 34333865
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human BDNF. The exact immunogen used to generate this antibody is proprietary information. Database link P23560
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Tested | Tested |
| Cat | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Pig, Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Pig, Chimpanzee | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Dilution is assay dependent. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Pig, Chimpanzee | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.BDNF precursor formImportant signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed:24908487, PubMed:29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).
Alternative names
Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF
Recommended products
Rabbit Polyclonal BDNF antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human BDNF.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human BDNF. The exact immunogen used to generate this antibody is proprietary information. Database link P23560
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-BDNF antibody (ab226843)
12% SDS-PAGE.
Running: 80V, 15min; 140V, 40 min. Transfer: Semi-dry, 18 V, 60 min (NC embrane). Blocking: 5% non-fat milk in TBST, RT, 60 minutes. Washing: 5 ml TBST, 4 x 5 min. ECL exposure.
All lanes: Western blot - Anti-BDNF antibody (ab226843) at 1/500 dilution
Lane 1: Mouse brain at 50 µg
Lane 2: Rat brain at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 27 kDa
Observed band size: 17 kDa
Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (ab226843)
Immunocytochemistry/ Immunofluorescence analysis of DIV9 rat E18 primary cortical neuron cells labeling BDNF with ab226843 at 1:500 (green). Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Cells were counterstained with DAPI (blue) and a Tau antibody diluted at 1/500 (yellow).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)
Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling BDNF with ab226843 at 1/500 dilution at 4°C overnight. Antigen Retrieval:Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30min. Blocking:1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 minutes. Secondary antibody incubation: ABC HRP Kit (Rabbit IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (ab226843)
Immunohistochemical analysis of paraffin-embedded adult mouse retina tissue labeling BDNF with ab226843 at 1/250 dilution (green). Counter nuclear stained with DAPI (blue) and a beta III Tubulin antibody diluted at 1/500 (red).
Western blot - Anti-BDNF antibody (ab226843)
15% SDS-PAGE
All lanes: Western blot - Anti-BDNF antibody (ab226843) at 1/1000 dilution
Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg
Lane 2: Transfected (+) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 27 kDa
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