Anti-BDNF antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (AB226843)

Immunohistochemical analysis of paraffin-embedded adult mouse retina tissue labeling BDNF with ab226843 at 1/250 dilution (green). Counter nuclear stained with DAPI (blue) and a beta III Tubulin antibody diluted at 1/500 (red).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (AB226843)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling BDNF with ab226843 at 1/500 dilution at 4°C overnight. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking : 3% H₂O₂, RT, 30min. Blocking : 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 minutes. Secondary antibody incubation : ABC HRP Kit (Rabbit IgG), 1 : 200, RT, 30min. Washing : PBS, 2 x 5 mins. Chromogen system : DAB.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (AB226843)

Paraffin-embedded mouse hippocampus tissue stained for BDNF protein at cytoplasm with ab226843 at a 1/1000 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min .

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (AB226843)

Paraffin-embedded mouse hippocampus tissue stained for BDNF protein at cytoplasm with ab226843 at a 1/1000 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (AB226843)

Paraffin-embedded rat hippocampus tissue stained for BDNF protein at cytoplasm with ab226843 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (AB226843)

Paraffin-embedded rat hippocampus tissue stained for BDNF protein at cytoplasm with ab226843 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody (AB226843)

Immunofluorescence analysis of 4% paraformaldehyde fixed at RT for 15 mins DIV10 rat E18 primary cortical neuron cells labelling BDNF protein using ab226843 at a 1/500 dilution (Green). Tau stained by anti- tau antibody at a 1/500 dilution (Red). The nuclear counterstain is DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (AB226843)

Paraffin-embedded rat brain tissue stained for BDNF protein at cytoplasm with ab226843 at a 1/1000 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody (AB226843)

Paraffin-embedded rat brain tissue stained for BDNF protein at cytoplasm with ab226843 at a 1/1000 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

CiteAb

Western blot - Anti-BDNF antibody (AB226843)

BDNF western blot using anti-BDNF antibody ab226843. Publication image and figure legend from Han, J., Oh, J. P., et al., 2019, Mol Brain, PubMed 31488185.

ab226843 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab226843 please see the product overview.

Ginsenoside F1 rescues pCREB and up-regulates BDNF expression level in APP/PS1 mice (a–d) Western blot showing expression levels of pCREB, CREB, and BDNF in the hippocampus. a Representative Western blot. b pCREB expression in WT mice (WT, n = 5), APP/PS1 mice with vehicle administration (AD-Vehicle, n = 5), and APP/PS1 mice with F1 administration (AD-F1, n = 5) (one-way ANOVA, F(2,12) = 7.623, P < 0.01; Tukey post hoc confirmed statistical significance between WT group and AD-Vehicle group, P < 0.01; AD-Vehicle group and AD-F1 group, P < 0.05). c CREB expression levels (WT, n = 3; AD-Vehicle, n = 3; AD-F1, n = 3) (one-way ANOVA, F(2,6) = 4.613, P = 0.0612). d BDNF expression levels (WT, n = 3; AD-Vehicle, n = 3; AD-F1, n = 3) (one-way ANOVA, F(2,6) = 0.4877, P = 0.6364). e–h Western blot showing expression levels of pCREB, CREB, and BDNF in the cortex. e Representative Western blot. f pCREB expression levels (WT, n = 3; AD-Vehicle, n = 3; AD-F1, n = 3) (one-way ANOVA, F(2,6) = 1.416, P = 0.3136). g CREB expression levels (WT, n = 3; AD-Vehicle, n = 3; AD-F1, n = 3) (one-way ANOVA, F(2,6) = 1.273, P = 0.3448). h BDNF expression levels (WT, n = 3; AD-Vehicle, n = 3; AD-F1, n = 3) (one-way ANOVA, F(2,6) = 7.331, P < 0.05; Tukey post hoc confirmed statistical significance between WT group and AD-F1 group, P < 0.05). The y-axis indicates normalized protein levels relative to the GAPDH control. n values indicate the number of mice. Data are mean ± s.e.m. *P < 0.05. **P < 0.01. Full-length blots are presented in additional files (See Additional file 1 and Additional file 2)

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