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Rabbit Recombinant Monoclonal BDNF antibody. Suitable for IHC-P, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 481 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFIHC-FrFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/500

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

For unpurified use at 1/100 - 1/250 dilution.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

For unpurified use at 1/750 dilution.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Species

Rat

Dilution info

1/100

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Species

Human

Dilution info

1/100

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.BDNF precursor formImportant signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed:24908487, PubMed:29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).

Alternative names

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Rabbit Recombinant Monoclonal BDNF antibody. Suitable for IHC-P, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 481 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR1292

Purification technique

Affinity purification Protein A

Specificity

This product may cross react with the following family members: NGF beta, neurotrophin 3, neurotrophin 4. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Western blot - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail

    Western blot - Anti-BDNF antibody [EPR1292] (ab108319)

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with unpurified ab108319 (1/1000) overnight at 4°C. Ab8245 (mouse anti-GAPDH; 0.05 ug/mL) was included as a loading control. Antibody binding was detected using goat anti-rabbit IgG IR-680 (green) and goat anti-mouse IgG IR800 (red) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

    All lanes: Western blot - Anti-BDNF antibody [EPR1292] (AB108319) at 1/1000 dilution

    Lane 1: Human hippocampus lysate at 20 µg

    Lane 2: Rat hippocampus lysate at 20 µg

    Lane 3: Mouse hippocampus lysate at 20 µg

    Secondary

    All lanes: Gt anti Rb IR680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 27 kDa

    Observed band size: 15 kDa, 28 kDa, 35 kDa, 45 kDa

  • Western blot - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail

    Western blot - Anti-BDNF antibody [EPR1292] (ab108319)

    All lanes: Western blot - Anti-BDNF antibody [EPR1292] (AB108319) at 1/1000 dilution

    Lane 1: Human brain lysates at 20 µg

    Lane 2: Mouse brain lysates at 20 µg

    Lane 3: Rat brain lysates at 20 µg

    Lane 4: Human hippocampus lysates at 20 µg

    Lane 5: Mouse hippocampus lysates at 20 µg

    Lane 6: Rat hippocampus lysates at 20 µg

    Lane 7: Human cerebellum lysates at 20 µg

    Lane 8: Mouse cerebellum lysates at 20 µg

    Lane 9: Rat cerebellum lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 27 kDa

    Observed band size: 15-45 kDa

  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)

    IHC image of BDNF staining in a section of frozen normal human cerebral cortex performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab108319, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)

    Immunohistochemistry (Frozen sections) analysis of rat cerebral cortex tissue sections labeling BDNF with Purified ab108319 at 1/100 (2.8 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling BDNF with Purified ab108319 at 1:500 dilution (0.56 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

  • Flow Cytometry (Intracellular) - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-BDNF antibody [EPR1292] (ab108319)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with purified ab108319 at 1/30 dilution (10μg/ml) (red). Cells were fixed with 80% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail

    Western blot - Anti-BDNF antibody [EPR1292] (ab108319)

    Different batches of ab108319 were tested on Mouse brain lysate at 0.3 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 14-45 kDa.

    All lanes: Western blot - Anti-BDNF antibody [EPR1292] (AB108319)

    Predicted band size: 27 kDa

  • Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] (ab108319)

    Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling BDNF with Purified ab108319 at 1/100 (2.8 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] (ab108319)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with Purified ab108319 at 1:500 (0.6 μg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319)

    Immunohistochemical analysis of paraffin-embedded human brain tissue using unpurified ab108319 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail
    This image is courtesy of a customer review submitted by Kirk McManus (1894904)

    Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] (ab108319)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling BDNF with unpurified ab108319 at a dilution of 1/750. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton-X100 in PBS. ab150081 (1/200) was used as the secondary antibody.

    The antibody produces a strong, golgi-associated labelling pattern in both PF and MeOH fixed samples.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] (ab108319)

    Immunohistochemical analysis of formalin fixed paraffin embedded human brain (cerebrum) labelling BDNF with ab108319 at a concentration of 0.56 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab108319 Anti-BDNF antibody [EPR1292] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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