Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free

12 product images

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  Western blot - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BDNF antibody [EPR1292] ab108319).

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with unpurified Anti-BDNF antibody [EPR1292] ab108319 (1/1000) overnight at 4°C. Anti-GAPDH antibody [6C5] - Loading Control ab8245 (mouse anti-GAPDH; 0.05 ug/mL) was included as a loading control. Antibody binding was detected using goat anti-rabbit IgG IR-680 (green) and goat anti-mouse IgG IR800 (red) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

  All lanes: Western blot - Anti-BDNF antibody [EPR1292] (Anti-BDNF antibody [EPR1292] ab108319) at 1/1000 dilution

  Lane 1: Human hippocampus lysate at 20 µg

  Lane 2: Rat hippocampus lysate at 20 µg

  Lane 3: Mouse hippocampus lysate at 20 µg

  Secondary

  All lanes: Gt anti Rb IR680 at 1/10000 dilution

  Performed under reducing conditions.

  Predicted band size: 27 kDa

  Observed band size: 15 kDa, 28 kDa, 35 kDa, 45 kDa

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  Western blot - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  This data was developed using Anti-BDNF antibody [EPR1292] ab108319, the same antibody clone in a different buffer formulation.

  All lanes: Western blot - Anti-BDNF antibody [EPR1292] (Anti-BDNF antibody [EPR1292] ab108319) at 1/1000 dilution

  Lane 1: Human brain lysates at 20 µg

  Lane 2: Mouse brain lysates at 20 µg

  Lane 3: Rat brain lysates at 20 µg

  Lane 4: Human hippocampus lysates at 20 µg

  Lane 5: Mouse hippocampus lysates at 20 µg

  Lane 6: Rat hippocampus lysates at 20 µg

  Lane 7: Human cerebellum lysates at 20 µg

  Lane 8: Mouse cerebellum lysates at 20 µg

  Lane 9: Rat cerebellum lysates at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Predicted band size: 27 kDa

  Observed band size: 15-45 kDa

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  Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  Immunohistochemistry (Frozen sections) analysis of rat cerebral cortex tissue sections labeling BDNF with Purified Anti-BDNF antibody [EPR1292] ab108319 at 1/100 (2.8 μg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BDNF antibody [EPR1292] ab108319).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling BDNF with Purified Anti-BDNF antibody [EPR1292] ab108319 at 1:500 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BDNF antibody [EPR1292] ab108319).

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  Flow Cytometry (Intracellular) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with purified Anti-BDNF antibody [EPR1292] ab108319 at 1/30 dilution (10 μg/ml) (red). Cells were fixed with 80% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BDNF antibody [EPR1292] ab108319).

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  Western blot - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  This data was developed using Anti-BDNF antibody [EPR1292] ab108319, the same antibody clone in a different buffer formulation. Different batches of Anti-BDNF antibody [EPR1292] ab108319 were tested on Mouse brain lysate at 0.3 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 14-45 kDa.

  All lanes: Western blot - Anti-BDNF antibody [EPR1292] (Anti-BDNF antibody [EPR1292] ab108319)

  Predicted band size: 27 kDa

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  Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling BDNF with Purified Anti-BDNF antibody [EPR1292] ab108319 at 1/100 (2.8 μg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BDNF antibody [EPR1292] ab108319).

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  Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with Purified Anti-BDNF antibody [EPR1292] ab108319 at 1:500 (0.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BDNF antibody [EPR1292] ab108319).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  This IHC data was generated using the same anti-BDNF antibody clone, EPR1292, in a different buffer formulation (cat# Anti-BDNF antibody [EPR1292] ab108319).
  

  Immunohistochemical analysis of paraffin-embedded Human brain tissue using Anti-BDNF antibody [EPR1292] ab108319 at 1/100

  Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

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  This image is courtesy of a customer review submitted by Kirk McManus (1894904)

  Immunocytochemistry/ Immunofluorescence - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  This ICC/IF data was generated using the same anti-BDNF antibody clone, EPR1292, in a different buffer formulation (cat# Anti-BDNF antibody [EPR1292] ab108319).
  

  Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling BDNF with Anti-BDNF antibody [EPR1292] ab108319 at a dilution of 1/750. Cells were fixed with Paraformaldehyde and permeabilised with 0.5% Triton-X100 in PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 (1/200) was used as the secondary antibody.

  The antibody produces a strong, golgi-associated labelling pattern in both PF and MeOH fixed samples.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  This data was developed using the same antibody clone in a different buffer formulation (Anti-BDNF antibody [EPR1292] ab108319).

  Immunohistochemical analysis of formalin fixed paraffin embedded human brain (cerebrum) labelling BDNF with Anti-BDNF antibody [EPR1292] ab108319 at a concentration of 0.56 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). Anti-BDNF antibody [EPR1292] ab108319 Anti-BDNF antibody [EPR1292] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

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  Western blot - Anti-BDNF antibody [EPR1292] - Low endotoxin, Azide free (ab216443)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BDNF antibody [EPR1292] ab108319).

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 was used as a His tag loading control.

  All lanes: Western blot - Anti-BDNF antibody [EPR1292] (Anti-BDNF antibody [EPR1292] ab108319) at 1/1000 dilution

  Lane 1: 293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

  Lane 2: 293T cells transfected with a Human BDNF expression vector containing a His-tag whole cell lysate at 20 µg

  Lane 3: 293T cells transfected with a Human NGF expression vector containing a His-tag whole cell lysate at 20 µg

  Lane 4: 293T cells transfected with a Human NTF3 expression vector containing a His-tag whole cell lysate at 20 µg

  Lane 5: 293T cells transfected with a Human NTF4 expression vector containing a His-tag whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 35 kDa

  Exposure time: 1s