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AB324065

Anti-BDNF antibody [EPR29334-72] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal BDNF antibody. Carrier free. Suitable for sELISA and reacts with Human, Rat samples.

View Alternative Names

Neurotrophic factor BDNF precursor form, proBDNF, Brain-derived neurotrophic factor, Bdnf

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Sandwich ELISA - Anti-BDNF antibody [EPR29334-72] - BSA and Azide free (Capture) (AB324065)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-BDNF antibody [EPR29334-72] - BSA and Azide free (Capture) (AB324065)

Interpolated concentration of native BDNF was measured at different sample concentrations. Undiluted samples are as follows : human serum 100%, and human plasma (Citrate) 100%. Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-BDNF antibody [EPR29334-72] - BSA and Azide free (Capture) (AB324065)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-BDNF antibody [EPR29334-72] - BSA and Azide free (Capture) (AB324065)

Sandwich ELISA using Rat BDNF immunogen protein standard curve with the capture antibody ab324065 at 2 ug/mL and detector antibody ab324112 at 0.5 ug/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown.)

Sandwich ELISA - Anti-BDNF antibody [EPR29334-72] - BSA and Azide free (Capture) (AB324065)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-BDNF antibody [EPR29334-72] - BSA and Azide free (Capture) (AB324065)

Interpolated concentration of native BDNF was measured at different sample concentrations. Undiluted samples are as follows : rat serum 100%, and rat plasma 100%. Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29334-72

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB212166

Human BDNF ELISA Kit

SimpleStep

ELISA - Human BDNF ELISA Kit (AB212166)

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Reactivity data

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Product details

ab324065 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Rat BDNF. The recommended pair for sandwich ELISA is:

Capture: ab324065, Human/Rat BDNF Capture Antibody (unconjugated)

Detector: ab324112, Human/Rat BDNF Detector Antibody (unconjugated)

The reference range value is 15.6-1000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Important signaling molecule that activates signaling cascades downstream of NTRK2 (By similarity). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability (By similarity).. Neurotrophic factor BDNF precursor form. Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
See full target information Bdnf
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com