Rabbit Recombinant Monoclonal BDP / ARID3B antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ARID3B.
Images
![Immunoprecipitation - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (AB276048), expandable thumbnail](https://content.abcam.com/products/images/bdp-arid3b-antibody-008-bsa-and-azide-free-ab276048--immunoprecipitation-img35535.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (AB276048)")
![Western blot - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (AB276048), expandable thumbnail](https://content.abcam.com/products/images/bdp-arid3b-antibody-008-bsa-and-azide-free-ab276048--western-blot-img16537.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (AB276048)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human ARID3B. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IVW6
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 1-4 μL/mg of lysate |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Target data
Function
Transcription factor which may be involved in neuroblastoma growth and malignant transformation. Favors nuclear targeting of ARID3A.
Alternative names
AT-rich interactive domain-containing protein 3B, ARID domain-containing protein 3B, Bright and dead ringer protein, Bright-like protein, ARID3B, DRIL2, BDP
Recommended products
Rabbit Recombinant Monoclonal BDP / ARID3B antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ARID3B.
Alternative names
AT-rich interactive domain-containing protein 3B, ARID domain-containing protein 3B, Bright and dead ringer protein, Bright-like protein, ARID3B, DRIL2, BDP
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human ARID3B. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IVW6
- Carrier free
Yes
- Clone number
008
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048), expandable thumbnail](https://content.abcam.com/products/images/bdp-arid3b-antibody-008-bsa-and-azide-free-ab276048--immunoprecipitation-img35535.jpg "Immunoprecipitation - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)")
Immunoprecipitation - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)
ARID3B was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia lymphoblast cell line ) (lane A) and HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (lane B) whole cell lysate with 2 μL ab276048 and 15 μl of 50% Protein G agarose. Western blot was performed from the immunoprecipitate using ab276048 at 1/100 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibodyat 1/5000 dilution.
Developed using the odssey technique.
Performed under reducing conditions.All lanes: Immunoprecipitation - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)
Predicted band size: 61 kDa
![Western blot - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048), expandable thumbnail](https://content.abcam.com/products/images/bdp-arid3b-antibody-008-bsa-and-azide-free-ab276048--western-blot-img16537.jpg "Western blot - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)")
Western blot - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)
Developed using the Odyssey technique.
Performed under reducing conditions.
All lanes: Western blot - Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048) at 1/500 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 30 µg
Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 61 kDa
Downloads
Product protocols
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