AB114071

Anti-Beclin 1 antibody [2A4]

Name: Anti-Beclin 1 antibody [2A4] (ab114071) | Abcam
Brand: Abcam Limited
SKU: AB114071
Price: 510 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(33 Publications)

Mouse Monoclonal Beclin 1 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 33 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BECN1 aa 50-250.

View Alternative Names

GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

4 Images

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Western blot - Anti-Beclin 1 antibody [2A4] (AB114071)

All lanes:

Western blot - Anti-Beclin 1 antibody [2A4] (ab114071) at 1/500 dilution

Lane 1:

Hela cell lysate

Lane 2:

A431 cell lysate

Lane 3:

MCF7 cell lysate

Lane 4:

Raji cell lysate

Lane 5:

Jurkat cell lysate

Lane 6:

SKBR3 cell lysate

Predicted band size: 52 kDa

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Flow Cytometry - Anti-Beclin 1 antibody [2A4] (AB114071)

Flow Cyt

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Flow Cytometry - Anti-Beclin 1 antibody [2A4] (AB114071)

Flow cytometric analysis of RAJI cells using Beclin 1 mouse mAb (green) and negative control (purple).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [2A4] (AB114071)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [2A4] (AB114071)

Immunohistochemical analysis of Beclin 1 expression in paraffin embedded breast cancer tissues (left) and liver cancer tissues (right) using ab114071 at 1/200 dilution with DAB staining

CiteAb

Western blot - Anti-Beclin 1 antibody [2A4] (AB114071)

Beclin 1 western blot using anti-Beclin 1 antibody [2A4] ab114071. Publication image and figure legend from Di Fazio, P., Waldegger, P., et al., 2016, Oncotarget, PubMed 27058414.

ab114071 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab114071 please see the product overview.

Beclin1 involvement(A) Beclin1 protein distribution was determined by immunofluorescence in HepG2 and Hep3B cells. After treatment with 100 nM panobinostat, Beclin1 aggregates are clearly visible. Immunofluorescence analysis has been performed under identical settings. Nuclei were stained with Hoechst 33342. Magnification is 630× and scale bar represents 10 μm. (B) Western blot of Beclin1. Densitometry results were normalized to β-actin content and are expressed relative to untreated controls set at 1.0.

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2A4

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human BECN1 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

Q14457

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.

Biological function summary

The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.

Pathways

Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.

Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a central role in autophagy (PubMed : 18570871, PubMed : 21358617, PubMed : 23184933, PubMed : 23974797, PubMed : 25484083, PubMed : 28445460, PubMed : 37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways : PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20208530, PubMed : 20643123, PubMed : 23974797, PubMed : 26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed : 25275521). May play a role in antiviral host defense.. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.

See full target information BECN1

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Stem cells translational medicine 14: PubMed40558385

2025

Bone mesenchymal stem cells attenuate hepatic stellate cell activation and liver fibrosis through REDD1/autophagy pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tingjuan Huang,Lina Nie,Haichao Diao,Ziyi Shang,Qizhi Shuai,Jun Xu,Jun Xie

Frontiers in molecular biosciences 11:1432945 PubMed39411401

2024

Endoplasmic reticulum stress alters myelin associated protein expression and extracellular vesicle composition in human oligodendrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ethan D Evalt,Saranraj Govindaraj,Madison T Jones,Nesve Ozsoy,Han Chen,Ashley E Russell

Journal of enzyme inhibition and medicinal chemistry 39:2343352 PubMed38700244

2024

The activity of pyrazolo[4,3-][1,2,4]triazine and pyrazolo[4,3-]tetrazolo[1,5-][1,2,4]triazine sulphonamide derivatives in monolayer and spheroid breast cancer cell cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Szymanowska,Dominika Radomska,Robert Czarnomysy,Mariusz Mojzych,Katarzyna Kotwica-Mojzych,Krzysztof Bielawski,Anna Bielawska

JCI insight 9: PubMed38652543

2024

Endothelial HIFα/PDGF-B to smooth muscle Beclin1 signaling sustains pathological muscularization in pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima Z Saddouk,Andrew Kuzemczak,Junichi Saito,Daniel M Greif

International journal of urology : official journal of the Japanese Urological Association 29:1085-1094 PubMed35975690

2022

TP53 loss-of-function causes vulnerability to autophagy inhibition in aggressive prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhang,Xian-Li Song,Bin Yu,Lian-Chee Foong,Yu Shu,Chun-Wai Mai,Jing Hu,Baijun Dong,Wei Xue,Chee Wai Chua

Journal of cellular and molecular medicine 26:1606-1620 PubMed35106915

2022

Acetylshikonin induces autophagy-dependent apoptosis through the key LKB1-AMPK and PI3K/Akt-regulated mTOR signalling pathways in HL-60 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Di Wu,Yuan-Ying Zhang,Shu-Ying Yi,Bei-Bei Sun,Jing Lan,Han-Ming Jiang,Gang-Ping Hao

Experimental and therapeutic medicine 22:1422 PubMed34707704

2021

MicroRNA-106a-5p promotes the proliferation, autophagy and migration of lung adenocarcinoma cells by targeting LKB1/AMPK.

Applications

Unspecified application

Species

Unspecified reactive species

Yushan Zhou,Yuxuan Zhang,Yanli Li,Liqiong Liu,Zhidong Li,Yanhong Liu,Yi Xiao

International journal of biological sciences 17:2970-2983 PubMed34345220

2021

MicroRNA-216b targets to potentiate autophagy and apoptosis of breast cancer cells the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Liu,Ping Ye,Yuanyuan Ye,Baosan Han

Cell death & disease 12:449 PubMed33953166

2021

SOX2 promotes chemoresistance, cancer stem cells properties, and epithelial-mesenchymal transition by β-catenin and Beclin1/autophagy signaling in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Zhu,Shimiao Huang,Shengyuan Chen,Jiaxuan Chen,Zhiqing Wang,Yadong Wang,Haoxuan Zheng

Journal of Cancer 12:3515-3528 PubMed33995628

2021

Prognostic Value of Autophagy, Microsatellite Instability, and Mutations in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wang,Zhi Zhao,Jing Zhuang,Xinxin Wu,Zhizhong Wang,Bing Zhang,Ge Gao,Yinping Zhang,Caili Guo,Qingxin Xia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Beclin 1 antibody [2A4]

Western blot - Anti-Beclin 1 antibody [2A4] (AB114071)

Flow Cytometry - Anti-Beclin 1 antibody [2A4] (AB114071)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [2A4] (AB114071)

Western blot - Anti-Beclin 1 antibody [2A4] (AB114071)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (33)

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