Rabbit Polyclonal Beclin 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human BECN1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin.
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- Pharmaceutics 15:2023Breast Milk Mesenchymal Stem Cells and/or Derived Exosomes Mitigated Adenine-Induced Nephropathy via Modulating Renal Autophagy and Fibrotic Signaling Pathways and Their Epigenetic Regulations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTarek Khamis,Amira Ebrahim Alsemeh,Asma Alanazi,Asmaa Monir Eltaweel,Heba M Abdel-Ghany,Doaa M Hendawy,Adel Abdelkhalek,Mahmoud A Said,Heba H Awad,Basma Hamed Ibrahim,Dina Mohamed Mekawy,Corina Pascu,Crista Florin,Ahmed Hamed ArishaPubMed 37631363
- Chronic diseases and translational medicine 9:238-2492023Cyclophilin D as a potential therapeutic target of liver ischemia/reperfusion injury by mediating crosstalk between apoptosis and autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengjiao Yang,Zhihui Wang,Jin Xie,Md Reyad-Ul-Ferdous,Siying Li,Yongfeng SongPubMed 37711863
- Cell death discovery 9:152023Neuroprotective effect of astragalin via activating PI3K/Akt-mTOR-mediated autophagy on APP/PS1 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCui-Zhu Yang,Shu-Han Wang,Run-Heng Zhang,Jia-Hong Lin,Ying-Hong Tian,Ya-Qi Yang,Jing Liu,Yu-Xin MaPubMed 36681681
- Antioxidants (Basel, Switzerland) 11:2022β-Cell Autophagy Pathway and Endoplasmic Reticulum Stress Regulating-Role of Liposomal Curcumin in Experimental Diabetes Mellitus: A Molecular and Morphometric Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSafaa I Khater,Mohamed F Dowidar,Aya E Abdel-Aziz,Tarek Khamis,Naief Dahran,Leena S Alqahtani,Mohamed M M Metwally,Al-Sayed Al-Hady Abd-Elrahamn,Mohammed Alsieni,Manal E Alosaimi,Maram H Abduljabbar,Amany Abdel-Rahman MohamedPubMed 36552609
- Oxidative medicine and cellular longevity 2022:13309282022Neuroprotective Mechanism of Icariin on Hypoxic Ischemic Brain Damage in Neonatal Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengxia Wang,Xiaoxia Yang,Qian Zhou,Yingqi Guo,Yingxiu Chen,Linyang Song,Junhua Yang,Lixia Li,Li LuoPubMed 36425058
- Oxidative medicine and cellular longevity 2022:76551422022Naringin protects human nucleus pulposus cells against TNF--induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenchang Chen,Shang Gao,Huapeng Guan,Xin Zhang,Yuliang Gao,Youxiang Su,Yun Song,Yuehua Jiang,Nianhu LiPubMed 35265264
- TheScientificWorldJournal 2021:13933722021Treadmill Exercise Training Ameliorates Functional and Structural Age-Associated Kidney Changes in Male Albino Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeba R Salem,Manar A FariedPubMed 34887703
- Journal of cellular and molecular medicine 25:11198-112112021Reticulocalbin 1 is required for proliferation and migration of non-small cell lung cancer cells regulated by osteoblast-conditioned medium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaijing Fu,Rui Chen,Yue Wang,Yang Xu,Chun Xia,Bing ZhangPubMed 34747128
- International journal of environmental research and public health 18:2021Immunohistochemical Analysis of the Beclin-1 Expression Predicts the Progression of Oral Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIda Barca,Chiara Mignogna,Daniela Novembre,Francesco Ferragina,Maria Giulia CristofaroPubMed 34769649
- Experimental and therapeutic medicine 22:7372021Inhibition of the Wnt/β-catenin signaling pathway reduces autophagy levels in complement treated podocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaocheng Dong,Haoran Dai,Yu Gao,Zhendong Feng,Wenbin Liu,Fei Liu,Zihan Zhang,Fang Ma,Xinran Xie,Zebing Zhu,Weijing Liu,Baoli LiuPubMed 34055054
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BECN1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q14457
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/500 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Target data
Function
Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.
Alternative names
GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197
Recommended products
Rabbit Polyclonal Beclin 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human BECN1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BECN1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q14457
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.
- Biological function summary
The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.
- Pathways
Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.
- Associated diseases and disorders
Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Western blot - Anti-Beclin 1 antibody (ab217179)
All lanes: Western blot - Anti-Beclin 1 antibody (ab217179) at 1/300 dilution
All lanes: 293T lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution
Predicted band size: 52 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (ab217179)
Immunofluorescent analysis of SMMC7721 cells labeling Beclin 1 with ab217179 at 1/50 dilution followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Western blot - Anti-Beclin 1 antibody (ab217179)
All lanes: Western blot - Anti-Beclin 1 antibody (ab217179) at 1/200 dilution
Lane 1: mouse brain lysates
Lane 2: mouse intestine lysates
SecondaryAll lanes: Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 52 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (ab217179)
Immunofluorescent analysis of U138 cells labeling Beclin 1 with ab217179 at 1/100 dilution followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab217179)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody (Goat Anti-Rabbit IgG, Cy3 conjugated) at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab217179)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab217179)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat kidney tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Beclin 1 antibody (ab217179)
Beclin 1 western blot using anti-Beclin 1 antibody ab217179. Publication image and figure legend from Peng, X., Ji, C., et al., 2020, J Cell Mol Med, PubMed 31657132.
ab217179 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab217179 please see the product overview.
LncRNA TNRC6C‐AS1 silencing promotes the apoptosis and autophagy of TC cells through STK4/Hippo. A, The mRNA expression of Beclin1 in transfected SW579 cells normalized to GAPDH was determined by RT‐qPCR. B, Western blot analysis was used to determine the protein levels of Beclin1, LC3‐II/I and Casepase‐3 in SW579 cells normalized to GAPDH after transfection. C, The proliferation ability of SW579 cells in each group was tested by EdU assay. D, TUNEL method was used to detect the apoptosis rate of SW579 cells. E, MDC method was used to detect the number of autophagic vesicles of SW579 cells in each group. *P < .05 vs the blank group, #P < .05 vs the shSTK4 group. The comparison among multiple groups was analysed by one‐way ANOVA (mean ± standard deviation). Values were obtained from 3 independent experiments in triplicate
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