AB217179

Anti-Beclin 1 antibody

Name: Anti-Beclin 1 antibody (ab217179) | Abcam
Brand: Abcam Limited
SKU: AB217179
Price: 535 USD
Availability: InStock

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(25 Publications)

Rabbit Polyclonal Beclin 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 25 publications. Immunogen corresponding to Synthetic Peptide within Human BECN1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (AB217179)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (AB217179)

Immunofluorescent analysis of SMMC7721 cells labeling Beclin 1 with ab217179 at 1/50 dilution followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (AB217179)

Immunofluorescent analysis of U138 cells labeling Beclin 1 with ab217179 at 1/100 dilution followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB217179)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB217179)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat kidney tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB217179)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB217179)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody (Goat Anti-Rabbit IgG, Cy3 conjugated) at 1/200 dilution.

Supplier Data

Western blot - Anti-Beclin 1 antibody (AB217179)

All lanes:

Western blot - Anti-Beclin 1 antibody (ab217179) at 1/300 dilution

All lanes:

293T lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution

Predicted band size: 52 kDa

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Supplier Data

Western blot - Anti-Beclin 1 antibody (AB217179)

All lanes:

Western blot - Anti-Beclin 1 antibody (ab217179) at 1/200 dilution

Lane 1:

mouse brain lysates

Lane 2:

mouse intestine lysates

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

Predicted band size: 52 kDa

false

CiteAb

Western blot - Anti-Beclin 1 antibody (AB217179)

Beclin 1 western blot using anti-Beclin 1 antibody ab217179. Publication image and figure legend from Peng, X., Ji, C., et al., 2020, J Cell Mol Med, PubMed 31657132.

ab217179 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab217179 please see the product overview.

LncRNA TNRC6C-AS1 silencing promotes the apoptosis and autophagy of TC cells through STK4/Hippo. A, The mRNA expression of Beclin1 in transfected SW579 cells normalized to GAPDH was determined by RT-qPCR. B, Western blot analysis was used to determine the protein levels of Beclin1, LC3-II/I and Casepase-3 in SW579 cells normalized to GAPDH after transfection. C, The proliferation ability of SW579 cells in each group was tested by EdU assay. D, TUNEL method was used to detect the apoptosis rate of SW579 cells. E, MDC method was used to detect the number of autophagic vesicles of SW579 cells in each group. *p < .05 vs the blank group, #p < .05 vs the shSTK4 group. The comparison among multiple groups was analysed by one-way ANOVA (mean ± standard deviation). Values were obtained from 3 independent experiments in triplicate

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human BECN1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q14457

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.

Biological function summary

The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.

Pathways

Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.

Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Plays a central role in autophagy (PubMed : 18570871, PubMed : 21358617, PubMed : 23184933, PubMed : 23974797, PubMed : 25484083, PubMed : 28445460, PubMed : 37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways : PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20208530, PubMed : 20643123, PubMed : 23974797, PubMed : 26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed : 25275521). May play a role in antiviral host defense.. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.

See full target information BECN1

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0314974 PubMed39739966

2025

High glucose promotes macrophage switching to the M1 phenotype via the downregulation of STAT-3 mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhao,Yuteng Jiang,Fengmei Wang,Li Sun,Mengyuan Ding,Liyuan Zhang,Beibei Wu,Xiaoliang Zhang

JCI insight 9: PubMed38652543

2024

Endothelial HIFα/PDGF-B to smooth muscle Beclin1 signaling sustains pathological muscularization in pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima Z Saddouk,Andrew Kuzemczak,Junichi Saito,Daniel M Greif

Scientific reports 14:575 PubMed38182706

2024

Melatonin enhances captopril mediated cardioprotective effects and improves mitochondrial dynamics in male Wistar rats with chronic heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Sherein F El-Sayed,Amira Mohamed Abdelhamid,Shimaa Gamal ZeinElabdeen,Dalia Ibrahim El-Wafaey,Suzan M M Moursi

Pharmaceutics 15: PubMed37631363

2023

Breast Milk Mesenchymal Stem Cells and/or Derived Exosomes Mitigated Adenine-Induced Nephropathy via Modulating Renal Autophagy and Fibrotic Signaling Pathways and Their Epigenetic Regulations.

Applications

Unspecified application

Species

Unspecified reactive species

Tarek Khamis,Amira Ebrahim Alsemeh,Asma Alanazi,Asmaa Monir Eltaweel,Heba M Abdel-Ghany,Doaa M Hendawy,Adel Abdelkhalek,Mahmoud A Said,Heba H Awad,Basma Hamed Ibrahim,Dina Mohamed Mekawy,Corina Pascu,Crista Florin,Ahmed Hamed Arisha

Open medicine (Warsaw, Poland) 18:20230698 PubMed37415610

2023

Dihydroartemisinin ameliorates cerebral I/R injury in rats via regulating VWF and autophagy-mediated SIRT1/FOXO1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Duan,Junxia Wu

Chronic diseases and translational medicine 9:238-249 PubMed37711863

2023

Cyclophilin D as a potential therapeutic target of liver ischemia/reperfusion injury by mediating crosstalk between apoptosis and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjiao Yang,Zhihui Wang,Jin Xie,Md Reyad-Ul-Ferdous,Siying Li,Yongfeng Song

Cell death discovery 9:15 PubMed36681681

2023

Neuroprotective effect of astragalin via activating PI3K/Akt-mTOR-mediated autophagy on APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cui-Zhu Yang,Shu-Han Wang,Run-Heng Zhang,Jia-Hong Lin,Ying-Hong Tian,Ya-Qi Yang,Jing Liu,Yu-Xin Ma

Antioxidants (Basel, Switzerland) 11: PubMed36552609

2022

β-Cell Autophagy Pathway and Endoplasmic Reticulum Stress Regulating-Role of Liposomal Curcumin in Experimental Diabetes Mellitus: A Molecular and Morphometric Study.

Applications

Unspecified application

Species

Unspecified reactive species

Safaa I Khater,Mohamed F Dowidar,Aya E Abdel-Aziz,Tarek Khamis,Naief Dahran,Leena S Alqahtani,Mohamed M M Metwally,Al-Sayed Al-Hady Abd-Elrahamn,Mohammed Alsieni,Manal E Alosaimi,Maram H Abduljabbar,Amany Abdel-Rahman Mohamed

Oxidative medicine and cellular longevity 2022:1330928 PubMed36425058

2022

Neuroprotective Mechanism of Icariin on Hypoxic Ischemic Brain Damage in Neonatal Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mengxia Wang,Xiaoxia Yang,Qian Zhou,Yingqi Guo,Yingxiu Chen,Linyang Song,Junhua Yang,Lixia Li,Li Luo

Arthritis research & therapy 24:165 PubMed35804467

2022

Increased Wnt/β-catenin signaling contributes to autophagy inhibition resulting from a dietary magnesium deficiency in injury-induced osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijun Bai,Michael Z Miao,Hui Li,Yiqing Wang,Ruixue Hou,Ke He,Xuan Wu,Hongyu Jin,Chao Zeng,Yang Cui,Guanghua Lei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Beclin 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (AB217179)

Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (AB217179)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB217179)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB217179)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB217179)

Western blot - Anti-Beclin 1 antibody (AB217179)

Western blot - Anti-Beclin 1 antibody (AB217179)

Western blot - Anti-Beclin 1 antibody (AB217179)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (25)

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