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AB223372

Anti-Beclin 1 antibody

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(2 Publications )

Anti-Beclin 1 antibody (ab223372) is a rabbit polyclonal antibody detecting Beclin 1 in Western Blot, ICC/IF. Suitable for Human.

View Alternative Names

GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

3 Images
Western blot - Anti-Beclin 1 antibody (AB223372)
  • WB

Supplier Data

Western blot - Anti-Beclin 1 antibody (AB223372)

Blocking buffer : 5% milk in TBST.

Color Development : TMB solution.

Primary and secondary antibodies were incubated for 1 hour at room temperature.

All lanes:

Western blot - Anti-Beclin 1 antibody (ab223372) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 51 kDa

false

Exposure time: 12min

Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (AB223372)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (AB223372)

4% formaldehyde-fixed SK-N-BE cells stained for Beclin 1 (green) using ab223372 at 1/100 dilution in ICC/IF. Secondary Antibody : Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain : Phalloidin Texas Red F-Actin stain (red) at 1/1000 for 60 minutes at room temperature; DAPI (blue) nuclear stain at 1/5000 for 5 minutes at room temperature.

(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223372 (D) Merge.

Western blot - Anti-Beclin 1 antibody (AB223372)
  • WB

Supplier Data

Western blot - Anti-Beclin 1 antibody (AB223372)

Blocking buffer : 5% milk in TBST.

Color Development : TMB solution.

Primary and secondary antibodies were incubated for 1 hour at room temperature.

All lanes:

Western blot - Anti-Beclin 1 antibody (ab223372) at 1/1000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 51 kDa

false

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human BECN1. The exact immunogen used to generate this antibody is proprietary information.

Q14457

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Beclin 1 antibody (ab223372) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Beclin 1?
Anti-Beclin 1 (ab223372) specifically detects a band for Beclin 1 (UniProt: Q14457) at a molecular weight of 52kDa.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.
Biological function summary

The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.

Pathways

Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.

Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a central role in autophagy (PubMed : 18570871, PubMed : 21358617, PubMed : 23184933, PubMed : 23974797, PubMed : 25484083, PubMed : 28445460, PubMed : 37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways : PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20208530, PubMed : 20643123, PubMed : 23974797, PubMed : 26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed : 25275521). May play a role in antiviral host defense.. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.
See full target information BECN1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e25234 PubMed38375306

2024

EPO regulates the differentiation and homing of bone marrow mesenchymal stem cells through Notch1/Jagged pathway to treat pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Li,Chongyang Shen,Nianchi Wen,Yicen Han,Lu Guo

Biology 9: PubMed32756447

2020

Exercise Ameliorates Insulin Resistance of Type 2 Diabetes through Motivating Short-Chain Fatty Acid-Mediated Skeletal Muscle Cell Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Yang,Haiqi Lin,Wentao Lin,Xiaoyang Xu
View all publications
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Product promise

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