Name: Anti-Beclin 1 antibody
SKU: ab62557
Rating: 4 (7 reviews)

Anti-Beclin 1 antibody (ab62557) is a rabbit polyclonal antibody that is used to detect Beclin 1 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Over 290 publications

- Trusted since 2008

Images

Western blot - Anti-Beclin 1 antibody (AB62557), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS
Form: Liquid
Clonality: Polyclonal

Immunogen

Synthetic Peptide within Human BECN1. The exact immunogen used to generate this antibody is proprietary information. Database link Q14457

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IF	IHC-P	ICC/IF
Human	Tested	Expected	Tested	Tested
Mouse	Tested	Tested	Tested	Expected
Rat	Tested	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1-2 µg/mL	Notes After transfer, wash membrane in TBST. Incubate membrane with 5% non-fat dry milk in TBS, 4C, overnight.. Dilute ab62557 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab62557 for 1 h at room temperature. Wash 3 times x 10 min in washing buffer with gentle agitation. Dilute 1μL secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 h at rt. Wash 3 x 10 min in washing buffer with gentle agitation. Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS. Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS. Blocking Buffer: 5% non-fat dry milk, TBS. Wash Buffer (TBST): 125 mM NaCl, 25mM Tris pH 8.0, 0.1% Tween-20.
Species Rat	Dilution info 1-2 µg/mL	Notes After transfer, wash membrane in TBST. Incubate membrane with 5% non-fat dry milk in TBS, 4C, overnight.. Dilute ab62557 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab62557 for 1 h at room temperature. Wash 3 times x 10 min in washing buffer with gentle agitation. Dilute 1μL secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 h at rt. Wash 3 x 10 min in washing buffer with gentle agitation. Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS. Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS. Blocking Buffer: 5% non-fat dry milk, TBS. Wash Buffer (TBST): 125 mM NaCl, 25mM Tris pH 8.0, 0.1% Tween-20.
Species Human	Dilution info 1-2 µg/mL	Notes After transfer, wash membrane in TBST. Incubate membrane with 5% non-fat dry milk in TBS, 4C, overnight.. Dilute ab62557 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab62557 for 1 h at room temperature. Wash 3 times x 10 min in washing buffer with gentle agitation. Dilute 1μL secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 h at rt. Wash 3 x 10 min in washing buffer with gentle agitation. Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS. Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS. Blocking Buffer: 5% non-fat dry milk, TBS. Wash Buffer (TBST): 125 mM NaCl, 25mM Tris pH 8.0, 0.1% Tween-20.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 10-20 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 2-5 µg/mL	Notes -
Species Rat	Dilution info 2-5 µg/mL	Notes -
Species Human	Dilution info 2-5 µg/mL	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 10-20 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

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Target data

See full target information BECN1

Function

Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.

Alternative names

GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS
Form: Liquid
Clonality: Polyclonal
Immunogen: Synthetic Peptide within Human BECN1. The exact immunogen used to generate this antibody is proprietary information. Database link Q14457
Purification technique: Affinity purification Immunogen
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

What is this antibody validated in?

Anti-Beclin 1 antibody (ab62557) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Beclin 1?

Anti-Beclin 1 (ab62557) specifically detects a band for Beclin 1 (UniProt: Q14457) at a molecular weight of 52kDa.

Trusted by the scientific community

Anti-Beclin 1 (ab62557) was first used in a scientific publication in 2008 and has been cited over 290 times in peer-reviewed journals.

Reviewed by scientists

Anti-Beclin 1 (ab62557) has over 5 independent reviews from customers.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.

Biological function summary

The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.

Pathways

Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.

Associated diseases and disorders

Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.

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9 product images

Western blot - Anti-Beclin 1 antibody (ab62557)
Gel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
All lanes: Western blot - Anti-Beclin 1 antibody (ab62557) at 1 µg/mL
Lane 1: 293 human cell line at 15 µg
Lane 2: A549 human cell line at 15 µg
Lane 3: HeLa cell line at 15 µg
Lane 4: HepG2 cell line at 15 µg
Lane 5: K562 cell line at 15 µg
Lane 6: MCF7 cell line at 15 µg
Lane 7: SK-N-SH cell line at 15 µg
Secondary
All lanes: Rabbit IgG antibody (HRP) at 1/1000 dilution
Predicted band size: 52 kDa
Western blot - Anti-Beclin 1 antibody (ab62557)
Gel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
All lanes: Western blot - Anti-Beclin 1 antibody (ab62557) at 1 µg/mL
Lane 1: Mouse heart tissue at 15 µg
Lane 2: Mouse brain tissue at 15 µg
Lane 3: Mouse colon tissue at 15 µg
Secondary
All lanes: Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 52 kDa
Western blot - Anti-Beclin 1 antibody (ab62557)
Gel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
All lanes: Western blot - Anti-Beclin 1 antibody (ab62557) at 1 µg
Lane 1: Rat liver tissue at 15 µg
Lane 2: Rat heart tissue at 15 µg
Lane 3: Rat lung tissue at 15 µg
Lane 4: Rat brain tissue at 15 µg
Lane 5: Rat ovary tissue at 15 µg
Lane 6: Rat colon tissue at 15 µg
Secondary
All lanes: Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 52 kDa
Immunofluorescence - Anti-Beclin 1 antibody (ab62557)
ab62557 at 20 μg/mL staining Beclin 1 (green) in mouse brain tissue by Immunofluorescence. DAPI staining in blue.
Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (ab62557)
Beclin 1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Beclin 1 antibody
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Beclin-1 with ab62557 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)
Beclin 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse brain tissue using rabbit Anti-Beclin 1 antibody
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Beclin-1 antibody (ab62557) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)
Beclin 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat brain tissue using rabbit Anti-Beclin 1 antibody
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Beclin-1 antibody (ab62557) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)
Beclin 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat liver tissue using rabbit Anti-Beclin 1 antibody
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Beclin-1 antibody (ab62557) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)
Beclin 1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human kidney tissue using rabbit Anti-Beclin 1 antibody
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Beclin-1 antibody (ab62557) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.