AB62557

Anti-Beclin 1 antibody

Name: Anti-Beclin 1 antibody (ab62557) | Abcam
Brand: Abcam Limited
SKU: AB62557
Price: 515 USD
Availability: InStock
Rating: 4 (7 reviews)

(7 Reviews)

(363 Publications)

Anti-Beclin 1 antibody (ab62557) is a rabbit polyclonal antibody detecting Beclin 1 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Over 290 publications
- Trusted since 2008

View Alternative Names

GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

9 Images

Unknown

Western blot - Anti-Beclin 1 antibody (AB62557)

Gel : 10%

Running condition : 130V, 1.5hrs

Transfer condition : Wet, 250mA, 2 hrs (NC membrane)

Blocking condition : 5% non-fat milk in TBS at 4 overnight

Primary antibody incubation : RT, 1hr

Secondary antibody incubation : RT, 1hr

Washing condition : 5 ml TBST, 4 x 5min

Exposure system : GE ECL 10 sec

All lanes:

Western blot - Anti-Beclin 1 antibody (ab62557) at 1 µg/mL

Lane 1:

293 human cell line at 15 µg

Lane 2:

A549 human cell line at 15 µg

Lane 3:

HeLa cell line at 15 µg

Lane 4:

HepG2 cell line at 15 µg

Lane 5:

K562 cell line at 15 µg

Lane 6:

MCF7 cell line at 15 µg

Lane 7:

SK-N-SH cell line at 15 µg

Secondary

All lanes:

Rabbit IgG antibody (HRP) at 1/1000 dilution

Predicted band size: 52 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (AB62557)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (AB62557)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling Beclin-1 with ab62557 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB62557)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB62557)

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Beclin-1 antibody (ab62557) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB62557)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Beclin-1 antibody (ab62557) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB62557)

Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Beclin-1 antibody (ab62557) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Supplier Data

Immunofluorescence - Anti-Beclin 1 antibody (AB62557)

ab62557 at 20 μg/mL staining Beclin 1 (green) in mouse brain tissue by Immunofluorescence. DAPI staining in blue.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB62557)

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Beclin-1 antibody (ab62557) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Unknown

Western blot - Anti-Beclin 1 antibody (AB62557)

Gel : 10%

Running condition : 130V, 1.5hrs

Transfer condition : Wet, 250mA, 2 hrs (NC membrane)

Blocking condition : 5% non-fat milk in TBS at 4 overnight

Primary antibody incubation : RT, 1hr

Secondary antibody incubation : RT, 1hr

Washing condition : 5 ml TBST, 4 x 5min

Exposure system : GE ECL 10 sec

All lanes:

Western blot - Anti-Beclin 1 antibody (ab62557) at 1 µg

Lane 1:

Rat liver tissue at 15 µg

Lane 2:

Rat heart tissue at 15 µg

Lane 3:

Rat lung tissue at 15 µg

Lane 4:

Rat brain tissue at 15 µg

Lane 5:

Rat ovary tissue at 15 µg

Lane 6:

Rat colon tissue at 15 µg

Secondary

All lanes:

Rabbit IgG antibody (HRP) at 1/10000 dilution

Predicted band size: 52 kDa

false

Unknown

Western blot - Anti-Beclin 1 antibody (AB62557)

Gel : 10%

Running condition : 130V, 1.5hrs

Transfer condition : Wet, 250mA, 2 hrs (NC membrane)

Blocking condition : 5% non-fat milk in TBS at 4 overnight

Primary antibody incubation : RT, 1hr

Secondary antibody incubation : RT, 1hr

Washing condition : 5 ml TBST, 4 x 5min

Exposure system : GE ECL 10 sec

All lanes:

Western blot - Anti-Beclin 1 antibody (ab62557) at 1 µg/mL

Lane 1:

Mouse heart tissue at 15 µg

Lane 2:

Mouse brain tissue at 15 µg

Lane 3:

Mouse colon tissue at 15 µg

Secondary

All lanes:

Rabbit IgG antibody (HRP) at 1/10000 dilution

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IF, ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human BECN1. The exact immunogen used to generate this antibody is proprietary information.

Q14457

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IF" : {"fullname" : "Immunofluorescence", "shortname":"IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "After transfer, wash membrane in TBST. Incubate membrane with 5% non-fat dry milk in TBS, 4C, overnight..Dilute ab62557 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab62557 for 1 h at room temperature. Wash 3 times x 10 min in washing buffer with gentle agitation.Dilute 1μL secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 h at rt. Wash 3 x 10 min in washing buffer with gentle agitation.Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS. Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS. Blocking Buffer: 5% non-fat dry milk, TBS. Wash Buffer (TBST): 125 mM NaCl, 25mM Tris pH 8.0, 0.1% Tween-20.", "IF-species-checked": "guaranteed", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10-20 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "After transfer, wash membrane in TBST. Incubate membrane with 5% non-fat dry milk in TBS, 4C, overnight..Dilute ab62557 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab62557 for 1 h at room temperature. Wash 3 times x 10 min in washing buffer with gentle agitation.Dilute 1μL secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 h at rt. Wash 3 x 10 min in washing buffer with gentle agitation.Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS. Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS. Blocking Buffer: 5% non-fat dry milk, TBS. Wash Buffer (TBST): 125 mM NaCl, 25mM Tris pH 8.0, 0.1% Tween-20.", "IF-species-checked": "testedAndGuaranteed", "IF-species-dilution-info": "10-20 µg/mL", "IF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "After transfer, wash membrane in TBST. Incubate membrane with 5% non-fat dry milk in TBS, 4C, overnight..Dilute ab62557 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab62557 for 1 h at room temperature. Wash 3 times x 10 min in washing buffer with gentle agitation.Dilute 1μL secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 h at rt. Wash 3 x 10 min in washing buffer with gentle agitation.Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS. Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS. Blocking Buffer: 5% non-fat dry milk, TBS. Wash Buffer (TBST): 125 mM NaCl, 25mM Tris pH 8.0, 0.1% Tween-20.", "IF-species-checked": "guaranteed", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Beclin 1 antibody (ab62557) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Beclin 1?
Anti-Beclin 1 (ab62557) specifically detects a band for Beclin 1 (UniProt: Q14457) at a molecular weight of 52kDa.

Trusted by the scientific community
Anti-Beclin 1 (ab62557) was first used in a scientific publication in 2008 and has been cited over 290 times in peer-reviewed journals.

Reviewed by scientists
Anti-Beclin 1 (ab62557) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.

Biological function summary

The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.

Pathways

Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.

Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a central role in autophagy (PubMed : 18570871, PubMed : 21358617, PubMed : 23184933, PubMed : 23974797, PubMed : 25484083, PubMed : 28445460, PubMed : 37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways : PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20208530, PubMed : 20643123, PubMed : 23974797, PubMed : 26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed : 25275521). May play a role in antiviral host defense.. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.

See full target information BECN1

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Publications (363)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29484 PubMed40796594

2025

Cardamonin inhibits the growth and stemness of osteosarcoma stem cells by inducing autophagy and inhibiting the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Zhang,Yu Jin,Qiannan Ding,Yulei Li,Lixia Yang,Mengyun Li,Zuo Lv,Kaifang Wang,Maowei Yang,Haixin Zhang,Xiucheng Li

Acta biochimica et biophysica Sinica : PubMed40567051

2025

Therapeutic potential of targeting the NEDD4L-eEF1A1 axis in cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Qin,Xinyue Wang,Minghui Zhao,Yuehui Liu,Huiyi Hou,Tingting Wang,Yuhe Pei,Jingxin Zhang,Zhou Shen,Feixiang Wu,Lishuang Zheng,Jinghua Li,Zhiyu Ni,Jianhong Shi

Cancer medicine 14:e70916 PubMed40476478

2025

Hepatitis B Virus X Protein Upregulates SREBP2 to Modulate Autophagy in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyan Lin,Yongxu Lin,Yongzhu Huang,Mingrong Wang,Xiaoxia Xie,Weiqi Cai,Qilan Guo,Pingying Jiang,Yuanlin Qi,Dan Li

Frontiers in aging neuroscience 17:1568002 PubMed40256391

2025

Galangin reduces MPTP-induced dopamine neuron injury via the autophagy dependent-PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Huang,Qiaofeng Li,Jingyi Wu,Yingying He,Junwei Huang,Sipeng Xie,Canfeng Yang,Qingling Ruan,Zhongliu Zhou,Minzhen Deng

Discover oncology 16:445 PubMed40169423

2025

miR-221 is a prognostic marker and promotes the proliferation and migration of esophageal squamous cell carcinoma by inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Mei,Zilu Chen,Foxing Tan,YuHeng Zhou,Haolin Du,Renjun Gu,Yan Huang

Open life sciences 20:20251063 PubMed40092733

2025

IL-11 promotes Ang II-induced autophagy inhibition and mitochondrial dysfunction in atrial fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Qianyu Zhang,Yunjie Han,Jun Zhang,Nan Zheng

Cell death & disease 16:81 PubMed39922805

2025

USP4-mediated CENPF deubiquitylation regulated tumor metastasis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongdong Xie,Hanbin Lin,Yuecheng Wu,Yanan Yu,Xintong Liu,Yating Zheng,Xiaojie Wang,Jiashu Wu,Meifang Xu,Yuting Han,Qiongying Zhang,Yu Deng,Lin Lin,Yan Linzhu,Li Qingyun,Xinjian Lin,Ying Huang,Pan Chi

Heliyon 10:e33829 PubMed39816371

2025

Quercetin attenuated ropivacaine-induced neurotoxicity in SH-SY5Y cells through upregulating Pim1 and enhancing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghai Liu,Ling Ren,Yangyang Sun,Jing Yan,Gu Gong

Nutrients 16: PubMed39770904

2025

The Effect of Quercetin on Non-Alcoholic Fatty Liver Disease (NAFLD) and the Role of Beclin1, P62, and LC3: An Experimental Study.

Applications

Unspecified application

Species

Unspecified reactive species

Ioannis Katsaros,Maria Sotiropoulou,Michail Vailas,Fotini Papachristou,Paraskevi Papakyriakopoulou,Marirena Grigoriou,Nikolaos Kostomitsopoulos,Alexandra Giatromanolaki,Georgia Valsami,Alexandra Tsaroucha,Dimitrios Schizas

Journal of diabetes research 2024:7146054 PubMed39741964

2025

Autophagy Regulates Ferroptosis-Mediated Diabetic Liver Injury by Modulating the Degradation of ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Liangxiu Wu,Weicheng Lai,Lanlan Li,Sen Yang,Fengjuan Li,Chen Yang,Xiaobing Gong,Liangyan Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Beclin 1 antibody

Western blot - Anti-Beclin 1 antibody (AB62557)

Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (AB62557)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB62557)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB62557)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB62557)

Immunofluorescence - Anti-Beclin 1 antibody (AB62557)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (AB62557)

Western blot - Anti-Beclin 1 antibody (AB62557)

Western blot - Anti-Beclin 1 antibody (AB62557)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (363)

Product promise