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Anti-Beclin 1 antibody [EPR19662] (ab207612) is a rabbit monoclonal antibody that is used to detect Beclin 1 in Western Blot, IP. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with Beclin 1 knockout cell line validation


Images

Western blot - Anti-Beclin 1 antibody [EPR19662] (AB207612), expandable thumbnail
  • Western blot - Anti-Beclin 1 antibody [EPR19662] (AB207612), expandable thumbnail
  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (AB207612), expandable thumbnail
  • Western blot - Anti-Beclin 1 antibody [EPR19662] (AB207612), expandable thumbnail
  • Western blot - Anti-Beclin 1 antibody [EPR19662] (AB207612), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Rat
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.

Alternative names

GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

Recommended products

Anti-Beclin 1 antibody [EPR19662] (ab207612) is a rabbit monoclonal antibody that is used to detect Beclin 1 in Western Blot, IP. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with Beclin 1 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19662
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Beclin 1 antibody [EPR19662] (ab207612) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), in Human, Mouse, Rat samples.

What is the molecular weight of Beclin 1?


Anti-Beclin 1 [EPR19662] (ab207612) specifically detects a band for Beclin 1 (UniProt: Q14457) at a molecular weight of 52kDa.

Recommended positive controls


WB: Human fetal brain, fetal heart and fetal kidney lysates; HeLa, HepG2, HEK-293, MCF7, Jurkat, Raji, C6, RAW264.7, PC-12, NIH/3T3, A20 and C2C12 whole cell lysates; rat brain, kidney and spleen lysates; mouse brain, heart and spleen lysates.IP: HeLa whole cell lysate.

Trusted by the scientific community


Anti-Beclin 1 [EPR19662] (ab207612) was first used in a scientific publication in 2016 and has been cited over 270 times in peer-reviewed journals.

Reviewed by scientists


Anti-Beclin 1 [EPR19662] (ab207612) has over 10 independent reviews from customers.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed


The specificity of Anti-Beclin 1 antibody [EPR19662] (ab207612) has been confirmed by Western blot testing in BECN1 Knockout HeLa cells.



Other related products


We have a range of other formats of antibody clone [EPR19662] also available for your convenience:
ab207612, Carrier free - Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free ab227107, Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) ab289864, Carrier free - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) - BSA and Azide free ab289873, Anti-Beclin-1 antibody [EPR19662] - Chicken IgY (Chimeric) ab302669, Carrier free - Anti-Beclin-1 antibody [EPR19662] - Chicken IgY (Chimeric) - BSA and Azide free ab302670



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.

Biological function summary

The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.

Pathways

Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.

Associated diseases and disorders

Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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6 product images

  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612), expandable thumbnail

    Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 52 kDa

    Observed band size: 52 kDa

    Exposure time: 30s

  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612), expandable thumbnail

    Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 30 seconds; Lane 5: 15 seconds; Lane 6: 15 seconds.

    All lanes: Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

    Lane 3: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

    Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

    Lane 5: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

    Lane 6: Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa

    Observed band size: 52 kDa

  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612), expandable thumbnail

    Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)

    Beclin 1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab207612 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab207612 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10μg (Input).

    Lane 2: ab207612 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab207612 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)

    Predicted band size: 52 kDa

  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612), expandable thumbnail

    Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 5 seconds; Lane 5: 10 seconds; Lane 6: 30 seconds.

    Lanes 1 - 4: Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution

    Lanes 5 - 6: Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution

    Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 2: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 3: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 4: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Lane 5: A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 10 µg

    Lane 6: C2C12 (Mouse myoblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/100000 dilution

    Predicted band size: 52 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612), expandable thumbnail

    Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/6: 5 seconds; Lane 2/3: 15 seconds; Lane 4: 3 seconds; Lane 5: 10 seconds.

    All lanes: Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution

    Lane 1: Rat brain lysate at 10 µg

    Lane 2: Rat kidney lysate at 10 µg

    Lane 3: Rat spleen lysate at 10 µg

    Lane 4: Mouse brain lysate at 10 µg

    Lane 5: Mouse heart lysate at 10 µg

    Lane 6: Mouse spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612), expandable thumbnail

    Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

    Western blot: Anti-BECN1 antibody [EPR19662] (ab207612) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab207612 was shown to bind specifically to BECN1. A band was observed at 57 kDa in wild-type HeLa cell lysates with no signal observed at this size in BECN1 knockout cell line. To generate this image, wild-type and BECN1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: BECN1 knockout HeLa cell lysate at 20 µg

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: HepG2 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

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