AB289864

Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric)

20ul selling size
Recombinant
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Mouse Recombinant Monoclonal Beclin 1 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

View Alternative Names

GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

5 Images

Lab

Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864)

Beclin 1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab289864 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289864 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg Lane 2 : ab289864 IP in HeLa whole cell lysate Lane 3 : Mouse monoclonal IgG2a (ab18413) instead of ab289864 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 23 seconds. Observed MW (kDa) : 52.

Lab

Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864)

Beclin 1 was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate with ab289864 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab289864 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate 10 µg Lane 2 : ab289864 IP in PC-12 whole cell lysate Lane 3 : Mouse monoclonal IgG2a (ab18413) instead of ab289864 in PC-12 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 75 seconds. Observed MW (kDa) : 52.

Lab

Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864)

Beclin 1 was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate with ab289864 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289864 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution. Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate 10 µg Lane 2 : ab289864 IP in RAW264.7 whole cell lysate Lane 3 : Mouse monoclonal IgG2a (ab18413) instead of ab289864 in RAW264.7 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 75 seconds. Observed MW (kDa) : 52.

Lab

Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : Lane 1 : 126 seconds Lane : 2, 4 : 3 minutes Lane 3, 5 : 15 seconds.

All lanes:

Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Rat heart tissue lysate at 20 µg

Lane 5:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-fc-hrp-preadsorbed-ab98717'>ab98717</a>) at 1/20000 dilution

Predicted band size: 51 kDa

Observed band size: 52 kDa

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Lab

Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : Lane 1-5, 9 : 15 seconds Lane 6-8 : 5.5 seconds

All lanes:

Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 6:

A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate at 20 µg

Lane 7:

C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

Lane 8:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 9:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysa at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-fc-hrp-preadsorbed-ab98717'>ab98717</a>) at 1/20000 dilution

Predicted band size: 51 kDa

Observed band size: 52 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EPR19662

Isotype

IgG2a

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab289864 does not react with mouse in immunoprecipitation.

This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab207612). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.

Biological function summary

The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.

Pathways

Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.

Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a central role in autophagy (PubMed : 18570871, PubMed : 21358617, PubMed : 23184933, PubMed : 23974797, PubMed : 25484083, PubMed : 28445460, PubMed : 37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways : PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20208530, PubMed : 20643123, PubMed : 23974797, PubMed : 26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed : 25275521). May play a role in antiviral host defense.. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.

See full target information BECN1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric)

Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864)

Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864)

Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864)

Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864)

Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise