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Mouse Recombinant Monoclonal Beclin 1 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864), expandable thumbnail
  • Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864), expandable thumbnail
  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864), expandable thumbnail
  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864), expandable thumbnail
  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (AB289864), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIP
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.

Alternative names

GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

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Mouse Recombinant Monoclonal Beclin 1 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19662
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab289864 does not react with mouse in immunoprecipitation.

This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Beclin 1 antibody [EPR19662] ab207612). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.

Biological function summary

The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.

Pathways

Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.

Associated diseases and disorders

Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864), expandable thumbnail

    Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

    Exposure time: Lane 1: 126 seconds Lane: 2, 4: 3 minutes Lane 3, 5: 15 seconds. 

    All lanes: Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Lane 4: Rat heart tissue lysate at 20 µg

    Lane 5: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (Goat Anti-Mouse IgG Fc (HRP) preadsorbed ab98717) at 1/20000 dilution

    Predicted band size: 51 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864), expandable thumbnail

    Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

    Exposure time: Lane 1-5, 9: 15 seconds Lane 6-8: 5.5 seconds

    All lanes: Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 4: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 5: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Lane 6: A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate at 20 µg

    Lane 7: C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

    Lane 8: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

    Lane 9: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysa at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (Goat Anti-Mouse IgG Fc (HRP) preadsorbed ab98717) at 1/20000 dilution

    Predicted band size: 51 kDa

    Observed band size: 52 kDa

  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864), expandable thumbnail

    Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)

    Beclin 1 was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate with ab289864 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab289864 at 1/1000 dilution. Mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/5000 dilution.

    Lane 1: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate 10 µg

    Lane 2: ab289864 IP in PC-12 whole cell lysate

    Lane 3: Mouse monoclonal IgG2a (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of ab289864 in PC-12 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 75 seconds. 

    Observed MW (kDa): 52. 

  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864), expandable thumbnail

    Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)

    Beclin 1 was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate with ab289864 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289864 at 1/1000 dilution. Mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/5000 dilution.

    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate 10 µg

    Lane 2: ab289864 IP in RAW264.7 whole cell lysate

    Lane 3: Mouse monoclonal IgG2a (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of ab289864 in RAW264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 75 seconds. 

    Observed MW (kDa): 52. 

  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864), expandable thumbnail

    Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (ab289864)

    Beclin 1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab289864 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289864 at 1/1000 dilution. Mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg

    Lane 2: ab289864 IP in HeLa whole cell lysate

    Lane 3: Mouse monoclonal IgG2a (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of ab289864 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 23 seconds. 

    Observed MW (kDa): 52.

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