Anti-Beclin 1 antibody [EPR20473] 20 ul size (ab210498)

Rabbit Recombinant Monoclonal Beclin 1 antibody. Suitable for WB, IP, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 136 publications.

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Images

Western blot - Anti-Beclin 1 antibody [EPR20473] (AB210498), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt	WB	IP	IHC-P
Human	Not recommended	Tested	Tested	Tested
Mouse	Not recommended	Tested	Expected	Expected
Rat	Not recommended	Tested	Expected	Expected

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes -
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information BECN1

Function

Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.

Alternative names

GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

Recommended products

Rabbit Recombinant Monoclonal Beclin 1 antibody. Suitable for WB, IP, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 136 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR20473
Purification technique: Affinity purification Protein A
Specificity: We don’t recommend this antibody for mouse and rat in IHC. In our hands most mouse and rat tissues showed nuclear staining.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.

Biological function summary

The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.

Pathways

Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.

Associated diseases and disorders

Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.

Product promise

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10 product images

Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 3: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 4: T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg
Lane 5: Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 15s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] (ab210498)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Beclin 1 with ab210498 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic staining on human kidney is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
We don't recommend this antibody for mouse and rat in IHC. In our hands most mouse and rat tissues showed nuclear staining.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] (ab210498)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Beclin 1 with ab210498 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic staining on tumor cells of human breast cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
We don't recommend this antibody for mouse and rat in IHC. In our hands most mouse and rat tissues showed nuclear staining.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Beclin 1 antibody [EPR20473] (ab210498)
Beclin 1 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab210498 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab210498 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HepG2 whole cell lysate, 10 μg (Input).
Lane 2: ab210498 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab210498 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-Beclin 1 antibody [EPR20473] (ab210498)
Predicted band size: 52 kDa
Observed band size: 52 kDa
Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Human colon lysate at 10 µg
Secondary
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 3min
Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498) at 1/1000 dilution
Lane 1: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Lane 3: A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 10 µg
Lane 4: C2C12 (Mouse myoblast cell line) whole cell lysate
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 1min
Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse spleen lysate at 10 µg
Lane 3: Rat brain lysate at 10 µg
Lane 4: Rat kidney lysate at 10 µg
Lane 5: Rat spleen lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 3min
Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498)
False colour image of Western blot: Anti-Beclin 1 antibody [EPR20473] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab210498 was shown to bind specifically to Beclin 1. A band was observed at 52 kDa in wild-type Jurkat cell lysates with no signal observed at this size in BECN1 CRISPR-Cas9 edited cell line ab283778 (BECN1 CRISPR-Cas9 edited cell lysate ab283792). The band observed in the CRISPR-Cas9 edited lysate lane above 52 kDa is likely to represent Beclin 1 with an insertion. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and BECN1 CRISPR-Cas9 edited Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498) at 1/1000 dilution
Lane 1: Wild-type Jurkat cell lysate at 20 µg
Lane 2: BECN1 CRISPR-Cas9 edited Jurkat cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: Raji cell lysate at 20 µg
Secondary
Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] (ab210498)
Immunohistochemistry analysis of paraffin embedded sections of human hepatocellular carcinoma tissue staining Beclin 1 with ab210498 at 1/100 dilution and ready to use secondary Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Counterstained with hematoxylin. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on human hepatocellular carcinoma. The section was incubated with ab210498 at 4°C overnight.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498)
Beclin 1 western blot using anti-Beclin 1 antibody [EPR20473] ab210498. Publication image and figure legend from Chow, J. P. H., Cai, Y., et al., 2020, PLoS One, PubMed 32353864.

ab210498 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab210498 please see the product overview.
Expression of ASS1 in various cell lines and the effect of NEI-01 on their cell viability.(A) Expression of ASS1, ASL and OTC in SMMC7721, MIA PaCa-2 and PANC-1. Unperturbed asynchronously growing cells were harvested by trypsinization. After cell lysis, total cell lysates were subject to immunoblot with anti-ASS1, anti-ASL, anti-OTC or anti-ACTIN antibodies. (B) Inhibition of cell growth of MIA PaCa-2 and PANC-1 cells but not SMMC7721 cells by NEI-01 treatment. SMMC7721 cells, MIA PaCa-2 cells or PANC-1 were seeded in 96 well-plate with ~3000 cells/well density 24 hr prior to NEI-01 treatments. After 72 hr, cells were subject to MTT assay. The relative cell viability in % vs the NEI-01 concentration was shown as mean ± SD of triplicate wells. (C) Activation of autophagic pathway upon NEI-01 treatment. MIA PaCa-2 cells were treated with indicated amount of NEI-01 for 3 or 7 days. The cells were then harvested and subject to immunoblot using designated antibodies.