Rabbit Recombinant Monoclonal Beclin 1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (AB232461), expandable thumbnail](https://content.abcam.com/products/images/beclin-1-antibody-epr20473-bsa-and-azide-free-ab232461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79635.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (AB232461)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (AB232461), expandable thumbnail](https://content.abcam.com/products/images/beclin-1-antibody-epr20473-bsa-and-azide-free-ab232461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72184.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (AB232461)")
![Immunoprecipitation - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (AB232461), expandable thumbnail](https://content.abcam.com/products/images/beclin-1-antibody-epr20473-bsa-and-azide-free-ab232461--immunoprecipitation-img70260.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (AB232461)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (AB232461), expandable thumbnail](https://content.abcam.com/products/images/beclin-1-antibody-epr20473-bsa-and-azide-free-ab232461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423569.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (AB232461)")
![Western blot - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (AB232461), expandable thumbnail](https://content.abcam.com/products/images/beclin-1-antibody-epr20473-bsa-and-azide-free-ab232461--western-blot-img417983.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (AB232461)")
Publications
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- Experimental and therapeutic medicine 21:4302021Dynamic study into autophagy and apoptosis during orthodontic tooth movement.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaoying Wang,Li Zhang,Fuwei Lin,Qian Zheng,Xiaomei Xu,Li MeiPubMed 33747169
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Tested |
| Mouse | Predicted | Not recommended | Expected | Predicted |
| Rat | Predicted | Not recommended | Expected | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
12 products for Alternative Product
Target data
Function
Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.
Alternative names
GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197
Recommended products
Rabbit Recombinant Monoclonal Beclin 1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20473
- Purification technique
- Affinity purification Protein A
- Specificity
We don’t recommend this antibody for mouse and rat in IHC. In our hands most mouse and rat tissues showed nuclear staining.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232461 is the carrier-free version of Anti-Beclin 1 antibody [EPR20473] ab210498.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.
- Biological function summary
The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.
- Pathways
Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.
- Associated diseases and disorders
Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461), expandable thumbnail](https://content.abcam.com/products/images/beclin-1-antibody-epr20473-bsa-and-azide-free-ab232461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img79635.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Beclin 1 with Anti-Beclin 1 antibody [EPR20473] ab210498 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic staining on human kidney is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
We don't recommend this antibody for mouse and rat in IHC. In our hands most mouse and rat tissues showed nuclear staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Beclin 1 antibody [EPR20473] ab210498).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461), expandable thumbnail](https://content.abcam.com/products/images/beclin-1-antibody-epr20473-bsa-and-azide-free-ab232461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72184.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Beclin 1 with Anti-Beclin 1 antibody [EPR20473] ab210498 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Granularly cytoplasmic staining on tumor cells of human breast cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
We don't recommend this antibody for mouse and rat in IHC. In our hands most mouse and rat tissues showed nuclear staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Beclin 1 antibody [EPR20473] ab210498).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461), expandable thumbnail](https://content.abcam.com/products/images/beclin-1-antibody-epr20473-bsa-and-azide-free-ab232461--immunoprecipitation-img70260.jpg "Immunoprecipitation - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)")
Immunoprecipitation - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)
Beclin 1 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with Anti-Beclin 1 antibody [EPR20473] ab210498 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using Anti-Beclin 1 antibody [EPR20473] ab210498 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HepG2 whole cell lysate, 10 μg (Input).
Lane 2: Anti-Beclin 1 antibody [EPR20473] ab210498 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Beclin 1 antibody [EPR20473] ab210498 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Beclin 1 antibody [EPR20473] ab210498).
All lanes: Immunoprecipitation - Anti-Beclin 1 antibody [EPR20473] (Anti-Beclin 1 antibody [EPR20473] ab210498)
Predicted band size: 52 kDa
Observed band size: 52 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461), expandable thumbnail](https://content.abcam.com/products/images/beclin-1-antibody-epr20473-bsa-and-azide-free-ab232461--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423569.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)
Immunohistochemistry analysis of paraffin embedded sections of human hepatocellular carcinoma tissue staining Beclin 1 with Anti-Beclin 1 antibody [EPR20473] ab210498 at 1/100 dilution and ready to use secondary Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Counterstained with hematoxylin. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on human hepatocellular carcinoma. The section was incubated with Anti-Beclin 1 antibody [EPR20473] ab210498 at 4°C overnight.
This data was developed using the same antibody clone in a different buffer formulation (Anti-Beclin 1 antibody [EPR20473] ab210498).![Western blot - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461), expandable thumbnail](https://content.abcam.com/products/images/beclin-1-antibody-epr20473-bsa-and-azide-free-ab232461--western-blot-img417983.jpg "Western blot - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)")
Western blot - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)
False colour image of Western blot: Anti-Beclin 1 antibody [EPR20473] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Beclin 1 antibody [EPR20473] ab210498 was shown to bind specifically to Beclin 1. A band was observed at 52 kDa in wild-type Jurkat cell lysates with no signal observed at this size in BECN1 CRISPR-Cas9 edited cell line ab283778 (BECN1 CRISPR-Cas9 edited cell lysate ab283792). The band observed in the CRISPR-Cas9 edited lysate lane above 52 kDa is likely to represent Beclin 1 with an insertion. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and BECN1 CRISPR-Cas9 edited Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Beclin 1 antibody [EPR20473] (Anti-Beclin 1 antibody [EPR20473] ab210498) at 1/1000 dilution
Lane 1: Wild-type Jurkat cell lysate at 20 µg
Lane 2: BECN1 CRISPR-Cas9 edited Jurkat cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: Raji cell lysate at 20 µg
SecondaryLanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 52 kDa
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