Rabbit Polyclonal Beclin 1 phospho S234 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human BECN1 phospho S234.
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- Molecular and cellular biochemistry 478:1415-14252022The protective effects of melatonin in high glucose environment by alleviating autophagy and apoptosis on primary cortical neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijiao Xiong,Song Liu,Chaoming Liu,Tianting Guo,Zhihua Huang,Liangdong LiPubMed 36348200
- Annals of translational medicine 9:15452021miR-17-5p attenuates kidney ischemia-reperfusion injury by inhibiting the PTEN and BIM pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Ma,Lei Fu,Zihao Jia,Qiang Zhong,Zhongli Huang,Xianding Wang,Yu Fan,Tao Lin,Turun SongPubMed 34790751
- International journal of molecular medicine 43:1311-13202019Rapamycin‑induced miR‑30a downregulation inhibits senescence of VSMCs by targeting Beclin1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPan Tan et. al.PubMed 30747228
- Scientific reports 8:6152018SESN2 facilitates mitophagy by helping Parkin translocation through ULK1 mediated Beclin1 phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshish Kumar,Chandrima ShahaPubMed 29330382
- International journal of molecular medicine 39:559-5682017Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathway.
- International journal of molecular medicine 37:309-182015Electroacupuncture protects against ischemic stroke by reducing autophagosome formation and inhibiting autophagy through the mTORC1-ULK1 complex-Beclin1 pathway.Applications:WBReactive species:Unspecified reactive speciesWeilin Liu,Guanhao Shang,Shanli Yang,Jia Huang,Xiehua Xue,Yunjiao Lin,Yi Zheng,Xian Wang,Lulu Wang,Ruhui Lin,Jing Tao,Lidian ChenPubMed 26647915
- PloS one 9:e1015262014Molecular switch role of Akt in Polygonatum odoratum lectin-induced apoptosis and autophagy in human non-small cell lung cancer A549 cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BECN1 phospho S234. The exact immunogen used to generate this antibody is proprietary information. Database link Q14457
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Primates | Predicted |
| Sheep | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Cow, Dog, Primates | Dilution info - | Notes - |
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Target data
Function
Plays a central role in autophagy (PubMed:18570871, PubMed:21358617, PubMed:23184933, PubMed:23974797, PubMed:25484083, PubMed:28445460, PubMed:37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123, PubMed:23974797, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.
Alternative names
GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197
Recommended products
Rabbit Polyclonal Beclin 1 phospho S234 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human BECN1 phospho S234.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BECN1 phospho S234. The exact immunogen used to generate this antibody is proprietary information. Database link Q14457
- Purification technique
- Affinity purification Immunogen
- Specificity
Immunolabeling of the Beclin 1 band is decreased in the Beclin 1 alanine substitution mutant. Additional bands may be observed in non-immunoprecipitated lysates.
- Concentration
- Purification notes
ab183335 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.
- Biological function summary
The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.
- Pathways
Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.
- Associated diseases and disorders
Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Beclin 1 (phospho S234) antibody (ab183335)
Beclin 1 (phospho S234) Western blot staining using rabbit Anti-Beclin 1 (phospho S234) antibody
All lanes: Western blot - Anti-Beclin 1 (phospho S234) antibody (ab183335) at 1/250 dilution
Lane 1: Lysate from 293T cells expressing wild type flag-Beclin 1
Lane 2: Lysate from 293T cells expressing mutant flag-Beclin 1 (S234A, S295A)
Predicted band size: 52 kDa
Observed band size: 60 kDa
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