Rabbit Recombinant Monoclonal BEND3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Recombinant fragment - Human samples.
Images
![Western blot - Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free (AB273577), expandable thumbnail](https://content.abcam.com/products/images/bend3-antibody-epr21038-60-bsa-and-azide-free-ab273577--western-blot-img42804.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free (AB273577)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant fragment - Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
Target data
Function
Transcriptional repressor which associates with the NoRC (nucleolar remodeling complex) complex and plays a key role in repressing rDNA transcription. The sumoylated form modulates the stability of the NoRC complex component BAZ2A/TIP5 by controlling its USP21-mediated deubiquitination (PubMed:21914818, PubMed:26100909). Binds to unmethylated major satellite DNA and is involved in the recruitment of the Polycomb repressive complex 2 (PRC2) to major satellites (By similarity). Stimulates the ERCC6L translocase and ATPase activities (PubMed:28977671).
Alternative names
KIAA1553, BEND3, BEN domain-containing protein 3
Recommended products
Rabbit Recombinant Monoclonal BEND3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21038-60
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab273577 is the carrier-free version of Anti-BEND3 antibody [EPR21038-60] ab215708.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free (ab273577), expandable thumbnail](https://content.abcam.com/products/images/bend3-antibody-epr21038-60-bsa-and-azide-free-ab273577--western-blot-img42804.jpg "Western blot - Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free (ab273577)")
Western blot - Anti-BEND3 antibody [EPR21038-60] - BSA and Azide free (ab273577)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
50 kDa unknown bands are observed.
Exposure times: Lanes 1-3: 158 seconds; Lanes 4-8: 180 seconds .
Thise data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (Anti-BEND3 antibody [EPR21038-60] ab215708).
All lanes: Western blot - Anti-BEND3 antibody [EPR21038-60] (Anti-BEND3 antibody [EPR21038-60] ab215708) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 4: F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 5: U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate at 20 µg
Lane 6: NCCIT (human pluripotent embryonic carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 7: HeLa treated with 10 uM MG-132 for 4 hours, whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 100 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com