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AB14927

Anti-Bestrophin/BEST1 antibody

4

(6 Reviews)

|

(8 Publications)

Rabbit Polyclonal Bestrophin/BEST1 antibody. Suitable for ICC, IP, WB, ICC/IF and reacts with Mouse, Rat, Human, Cow samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human BEST1 aa 1-100.

View Alternative Names

VMD2, BEST1, Bestrophin-1, TU15B, Vitelliform macular dystrophy protein 2

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Cow, Human

Applications

WB, ICC, IP, ICC/IF

applications

Immunogen

Synthetic Peptide within Human BEST1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O76090

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Alternative Products

Primary Antibodies

AB2182

Anti-Bestrophin/BEST1 antibody [E6-6]

Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (AB2182)

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Primary Antibodies

AB14928

Anti-Bestrophin/BEST1 antibody

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Primary Antibodies

AB14929

Anti-Bestrophin/BEST1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bestrophin/BEST1 antibody (AB14929)

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB12039

Anti-MiTF antibody [C5]

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: Tris glycine, 30% Glycerol (glycerin, glycerine), 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bestrophin also known as BEST1 or VMD2 is a protein that functions as a calcium-activated chloride channel. It plays an important role in the transport of ions across cell membranes. The molecular mass of BEST1 is about 68 kDa. This protein is prominently expressed in the retinal pigment epithelium (RPE) which is a layer of cells in the eye vital to the function and survival of the photoreceptors. These photoreceptors are responsible for capturing light and initiating vision.
Biological function summary

BEST1 influences the regulation of ionic homeostasis in the eye and contributes to the RPE's maintenance. It acts as part of a chloride channel complex that helps transport chloride ions which is important for fluid absorption and secretion in the retina. There are interactions with calcium ions that activate the channel allowing it to regulate the flow of other ions in response to changes in calcium concentration which is essential for maintaining the ionic balance necessary for proper vision.

Pathways

BEST1 plays significant roles in the visual cycle and ion transport pathways in the retina. It is related to the protein ANO2 another chloride channel although they interact differently with calcium ions. BEST1 influences the fluid regulation within the retina an essential part of the visual cycle pathway. It signals the movement of ions in response to external stimuli maintaining the balance of fluids and ions that reh cells function correctly.

Mutations in the BEST1 gene are connected to Best vitelliform macular dystrophy and adult-onset foveomacular dystrophy. Both disorders lead to retinal degeneration affecting vision. The interaction between BEST1 and other chloride channels like ANO2 suggests possible effects on the severity and progression of these retinal diseases highlighting the need for continued research into therapeutic interventions and the complex network of protein interactions within the retina.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+) (PubMed : 11904445, PubMed : 12907679, PubMed : 18179881, PubMed : 18400985, PubMed : 19853238, PubMed : 21330666, PubMed : 26200502, PubMed : 26720466, PubMed : 35789156). Allows the movement of chloride and hydrogencarbonate (PubMed : 11904445, PubMed : 12907679, PubMed : 18179881, PubMed : 18400985, PubMed : 19853238, PubMed : 21330666, PubMed : 26200502, PubMed : 26720466, PubMed : 35789156). Found in a partially open conformation leading to significantly smaller chloride movement (PubMed : 35789156). Upon F2R/PAR-1 activation, the sequestered calcium is released into the cytosol of astrocytes, leading to the (Ca2+)-dependent release of L-glutamate into the synaptic cleft that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). Upon activation of the norepinephrine-alpha-1 adrenergic receptor signaling pathway, transports as well D-serine than L-glutamate in a (Ca2+)-dependent manner, leading to activation of adjacent NMDAR receptors and therefore regulates the heterosynaptic long-term depression and metaplasticity during initial memory acquisition (By similarity). Releases the 4-aminobutanoate neurotransmitter in a (Ca2+)-dependent manner, and participates in its tonic release from cerebellar glial cells (By similarity).
See full target information BEST1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 13:3008-3026 PubMed37521872

2023

Glutamate-releasing BEST1 channel is a new target for neuroprotection against ischemic stroke with wide time window.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Xiong,Hui Xiao,Meng Sun,Yunjie Liu,Ling Gao,Ke Xu,Haiying Liang,Nan Jiang,Yuhui Lin,Lei Chang,Haiyin Wu,Dongya Zhu,Chunxia Luo

Annals of translational medicine 9:245 PubMed33708872

2021

Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zhang,Bingnan Su,Luyan Jiao,Ze-Hua Xu,Chang-Jun Zhang,Jinfu Nie,Mei-Ling Gao,Ying V Zhang,Zi-Bing Jin

Frontiers in bioengineering and biotechnology 8:456 PubMed32500067

2020

Optimisation of a Novel Bio-Substrate as a Treatment for Atrophic Age-Related Macular Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel McCormick,Ian Pearce,Stephen Kaye,Atikah Haneef

The Journal of neuroscience : the official journal 37:6712-6728 PubMed28592694

2017

Opening a New Time Window for Treatment of Stroke by Targeting HDAC2.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hui Lin,Jian Dong,Ying Tang,Huan-Yu Ni,Yu Zhang,Ping Su,Hai-Ying Liang,Meng-Cheng Yao,Hong-Jin Yuan,Dong-Liang Wang,Lei Chang,Hai-Yin Wu,Chun-Xia Luo,Dong-Ya Zhu

PloS one 12:e0173575 PubMed28282420

2017

Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Zhouhui Geng,Patrick J Walsh,Vincent Truong,Caitlin Hill,Mara Ebeling,Rebecca J Kapphahn,Sandra R Montezuma,Ching Yuan,Heidi Roehrich,Deborah A Ferrington,James R Dutton

Scientific reports 6:21975 PubMed26911344

2016

The extracellular calcium-sensing receptor regulates human fetal lung development via CFTR.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah C Brennan,William J Wilkinson,Hsiu-Er Tseng,Brenda Finney,Bethan Monk,Holly Dibble,Samantha Quilliam,David Warburton,Luis J Galietta,Paul J Kemp,Daniela Riccardi

Investigative ophthalmology & visual science 52:4497-505 PubMed21498618

2011

Molecular consequences of BEST1 gene mutations in canine multifocal retinopathy predict functional implications for human bestrophinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Karina E Guziewicz,Julianna Slavik,Sarah J P Lindauer,Gustavo D Aguirre,Barbara Zangerl

The Journal of physiology 587:3777-93 PubMed19528248

2009

Claudin-16 affects transcellular Cl- secretion in MDCK cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dorothee Günzel,Salah Amasheh,Sandra Pfaffenbach,Jan F Richter,P Jaya Kausalya,Walter Hunziker,Michael Fromm
View all publications
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Product promise

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