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AB2182

Anti-Bestrophin/BEST1 antibody [E6-6]

5

(7 Reviews)

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(39 Publications)

Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) is a mouse monoclonal antibody detecting Bestrophin/BEST1 in Western Blot, IP, IHC-Fr, ICC/IF. Suitable for Cow, Dog, Human, Monkey, Pig.

- Over 30 publications
- Trusted since 2002

View Alternative Names

VMD2, BEST1, Bestrophin-1, TU15B, Vitelliform macular dystrophy protein 2

3 Images
Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (AB2182)
  • WB

AbReview9515****

Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (AB2182)

The primary antobody was diluted in PBS/Tween/5%Milk and incubated for 1.5 hours at 25°C.

All lanes:

Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) at 1/1000 dilution

Lane 1:

Human RPE, retinal pigment epithelial cell lysate at 20 µg

Lane 2:

Non transfected HEK 293 cell extract at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-mouse

Predicted band size: 68 kDa

Observed band size: 67 kDa

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Exposure time: 5min

This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (AB2182)
  • WB

Unknown

Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (AB2182)

All lanes:

Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) at 1/1000 dilution

All lanes:

Human RPE cell lysate

Predicted band size: 68 kDa

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Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (AB2182)
  • WB

CiteAb

Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (AB2182)

Bestrophin/BEST1 western blot using anti-Bestrophin/BEST1 antibody [E6-6] ab2182. Publication image and figure legend from Hazim, R. A., Karumbayaram, S., et al., 2017, Stem Cell Res Ther, PubMed 28969679.

ab2182 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab2182 please see the product overview.

Specific protein and gene expression profiling of iPSC-derived RPE cells. a Expression of RPE65 by 2.5–3-month cultures of RPE cells from three different iPSC lines, indicated by an immunolabeled western blot image. Actin was used as a loading control. b Expression of RPE65, MERTK, and BEST1 genes in iPSC-RPE cells cultured for 2–3 months, detected by RT-PCR. GAPDH was used as a loading control. c–e Expression of the RPE-specific proteins BEST1 (c), RPE65 (d), and MITF (e), indicated by immunofluorescence. f iPSC-RPE cells showed no expression of the pluripotency marker OCT4; nuclei labeled with DAPI. Scale bars : c, 20 μm; d, e, 50 μm; f, 20 μm. HEK human embryonic kidney cells, iPSC induced pluripotent stem cell, RPE retinal pigment epithelium, MERTK MER proto-oncogene, tyrosine kinase, BEST1 bestrophin 1, GAPDH glyceraldehyde-3-phosphate dehydrogenase, MITF microphthalmia-associated transcription factor

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

E6-6

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human, Monkey, Dog, Pig, Cow

Applications

IHC-Fr, IP, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human BEST1 aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O76090

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Reactivity data

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"IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Dog": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Goat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Monkey": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Pig": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Primates": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rabbit": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Dog, Human, Monkey, Pig samples.

Trusted by the scientific community
Anti-Bestrophin/BEST1 [E6-6] (ab2182) was first used in a scientific publication in 2002 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-Bestrophin/BEST1 [E6-6] (ab2182) has over 5 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bestrophin also known as BEST1 or VMD2 is a protein that functions as a calcium-activated chloride channel. It plays an important role in the transport of ions across cell membranes. The molecular mass of BEST1 is about 68 kDa. This protein is prominently expressed in the retinal pigment epithelium (RPE) which is a layer of cells in the eye vital to the function and survival of the photoreceptors. These photoreceptors are responsible for capturing light and initiating vision.
Biological function summary

BEST1 influences the regulation of ionic homeostasis in the eye and contributes to the RPE's maintenance. It acts as part of a chloride channel complex that helps transport chloride ions which is important for fluid absorption and secretion in the retina. There are interactions with calcium ions that activate the channel allowing it to regulate the flow of other ions in response to changes in calcium concentration which is essential for maintaining the ionic balance necessary for proper vision.

Pathways

BEST1 plays significant roles in the visual cycle and ion transport pathways in the retina. It is related to the protein ANO2 another chloride channel although they interact differently with calcium ions. BEST1 influences the fluid regulation within the retina an essential part of the visual cycle pathway. It signals the movement of ions in response to external stimuli maintaining the balance of fluids and ions that reh cells function correctly.

Mutations in the BEST1 gene are connected to Best vitelliform macular dystrophy and adult-onset foveomacular dystrophy. Both disorders lead to retinal degeneration affecting vision. The interaction between BEST1 and other chloride channels like ANO2 suggests possible effects on the severity and progression of these retinal diseases highlighting the need for continued research into therapeutic interventions and the complex network of protein interactions within the retina.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+) (PubMed : 11904445, PubMed : 12907679, PubMed : 18179881, PubMed : 18400985, PubMed : 19853238, PubMed : 21330666, PubMed : 26200502, PubMed : 26720466, PubMed : 35789156). Allows the movement of chloride and hydrogencarbonate (PubMed : 11904445, PubMed : 12907679, PubMed : 18179881, PubMed : 18400985, PubMed : 19853238, PubMed : 21330666, PubMed : 26200502, PubMed : 26720466, PubMed : 35789156). Found in a partially open conformation leading to significantly smaller chloride movement (PubMed : 35789156). Upon F2R/PAR-1 activation, the sequestered calcium is released into the cytosol of astrocytes, leading to the (Ca2+)-dependent release of L-glutamate into the synaptic cleft that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). Upon activation of the norepinephrine-alpha-1 adrenergic receptor signaling pathway, transports as well D-serine than L-glutamate in a (Ca2+)-dependent manner, leading to activation of adjacent NMDAR receptors and therefore regulates the heterosynaptic long-term depression and metaplasticity during initial memory acquisition (By similarity). Releases the 4-aminobutanoate neurotransmitter in a (Ca2+)-dependent manner, and participates in its tonic release from cerebellar glial cells (By similarity).
See full target information BEST1
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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:27106 PubMed40715346

2025

Regulation of RPE65 expression in human retinal pigment epithelium cells.

Applications

Unspecified application

Species

Unspecified reactive species

Olga A Postnikova,Samuel William,Sheetal Uppal,Steven L Bernstein,Eugenia Poliakov,Igor B Rogozin,T Michael Redmond

Cells 14: PubMed40643526

2025

Porcine Single-Eye Retinal Pigment Epithelium Cell Culture for Barrier and Polarity Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp Dörschmann,Sina von der Weppen,Emi Koyama,Johann Roider,Alexa Klettner

JCI insight 10: PubMed40327408

2025

A spontaneous nonhuman primate model of inherited retinal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yi,Mingming Xu,Ying Xue,Yingxue Cao,Ziqi Yang,Lingli Zhou,Yang Zhou,Le Shi,Xiaomei Mai,Zehui Sun,Wenjie Qing,Yuying Li,Aolun Qing,Kaiwen Zhang,Lechun Ou,Shoudeng Chen,Elia J Duh,Xialin Liu

Scientific reports 14:22391 PubMed39333742

2024

Clustered ARPE-19 cells distinct in mitochondrial membrane potential may play a pivotal role in cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Takafumi Miyatani,Hiroshi Tanaka,Kosaku Numa,Asako Uehara,Yohei Otsuki,Junji Hamuro,Shigeru Kinoshita,Chie Sotozono

The Journal of biological chemistry 300:107569 PubMed39009342

2024

Glycogen myophosphorylase loss causes increased dependence on glucose in iPSC-derived retinal pigment epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Basudha Basu,Magdalena Karwatka,Becky China,Martin McKibbin,Kamron Khan,Chris F Inglehearn,John E Ladbury,Colin A Johnson

Gels (Basel, Switzerland) 10: PubMed38786253

2024

Sequential Fabrication of a Three-Layer Retina-like Structure.

Applications

Unspecified application

Species

Unspecified reactive species

Yahel Shechter,Roni Cohen,Michael Namestnikov,Assaf Shapira,Adiel Barak,Aya Barzelay,Tal Dvir

Proceedings of the National Academy of Sciences of the United States of America 120:e2214842120 PubMed37339216

2023

Single-cell transcriptomics reveals maturation of transplanted stem cell-derived retinal pigment epithelial cells toward native state.

Applications

Unspecified application

Species

Unspecified reactive species

Bhav Harshad Parikh,Paul Blakeley,Kakkad Regha,Zengping Liu,Binxia Yang,Mayuri Bhargava,Daniel Soo Lin Wong,Queenie Shu Woon Tan,Claudine See Wei Wong,Hao Fei Wang,Abdurrahmaan Al-Mubaarak,Chai Chou,Chui Ming Gemmy Cheung,Kah Leong Lim,Veluchamy Amutha Barathi,Walter Hunziker,Gopal Lingam,Tim Xiaoming Hu,Xinyi Su

Investigative ophthalmology & visual science 64:9 PubMed37163276

2023

Spatiotemporal Coordination of RPE Cell Quality by Extracellular Vesicle miR-494-3p Via Competitive Interplays With SIRT3 or PTEN.

Applications

Unspecified application

Species

Unspecified reactive species

Junji Hamuro,Tomoko Yamashita,Yohei Otsuki,Nao Hiramoto,Mayuka Adachi,Takafumi Miyatani,Hiroshi Tanaka,Morio Ueno,Shigeru Kinoshita,Chie Sotozono

International journal of molecular sciences 23: PubMed36499228

2022

Transient Retention of Photoreceptor Outer Segments in Matrigel-Embedded Retinal Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Berber,Sofiia Bondarenko,Lisa Michaelis,Bernhard Heinrich Friedrich Weber

Stem cell research & therapy 13:454 PubMed36064625

2022

Determining the optimal stage for cryopreservation of human embryonic stem cell-derived retinal pigment epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhang,Xianyu Huang,Sujun Liu,Xinyue Bai,Xinyue Zhu,Dennis O Clegg,Mei Jiang,Xiaodong Sun
View all publications
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