AB85037

Anti-beta 1 Adrenergic Receptor antibody

Name: Anti-beta 1 Adrenergic Receptor antibody (ab85037) | Abcam
Brand: Abcam Limited
SKU: AB85037
Rating: 3 (4 reviews)

(4 Reviews)

(4 Publications)

Rabbit Polyclonal beta 1 Adrenergic Receptor antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications.

View Alternative Names

Adrb1r, Beta-1 adrenergic receptor, Beta-1 adrenoreceptor, Beta-1 adrenoceptor

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-beta 1 Adrenergic Receptor antibody (AB85037)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-beta 1 Adrenergic Receptor antibody (AB85037)

ab85037 staining beta 1 Adrenergic Receptor in THP-1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab85037 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

Unknown

Western blot - Anti-beta 1 Adrenergic Receptor antibody (AB85037)

Mouse Beta-1 adrenergic receptor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-beta 1 Adrenergic Receptor antibody (ab85037) at 1 µg/mL

All lanes:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution

Predicted band size: 51 kDa

Observed band size: 55 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The beta 1 adrenergic receptor also known as beta-1 adrenoceptor or beta-1 receptor is a G protein-coupled receptor involved in the response to epinephrine and norepinephrine. It has a molecular mass of approximately 51 kDa. This receptor is mainly expressed in the heart where it plays a significant role in regulating cardiovascular functions. It is also found in other tissues like the brain and kidneys though at lower levels. The beta-1 adrenergic receptor functions by activating adenylate cyclase which increases intracellular cAMP levels.

Biological function summary

The beta 1 adrenergic receptor influences heart rate and cardiac contractility. This receptor is key in the sympathetic nervous system affecting physiological responses during stress or exercise. It does not form part of a complex in its active state. Upon activation the receptor enhances cardiac output by increasing the force and frequency of heart contractions. It also contributes to the relaxation of vascular and smooth muscle tissues facilitating better blood flow and pressure regulation.

Pathways

Beta 1 adrenergic receptor is integral to the adrenergic signaling pathway. It works alongside proteins like adenylate cyclase to modulate the levels of cAMP within the cell which acts as a secondary messenger in signal transduction. The receptor plays a significant role in the fight-or-flight response coordinating with other adrenergic receptors like the beta 2 adrenergic receptor to ensure a well-rounded physiological reaction to stressors. Another pathway of significance is the cardiac beta-adrenergic signaling which involves the regulation of heart muscle activities.

The beta 1 adrenergic receptor is associated with heart failure and hypertension. In heart failure altered expression or function of this receptor can lead to impaired contractile response and cardiac performance. In hypertension overactive adrenergic receptor signaling contributes to increased cardiac output and blood pressure. Related proteins include the beta-2 adrenergic receptor and angiotensin II which can exacerbate these conditions when dysregulated influencing the development and progression of cardiovascular diseases. Esmolol a beta-1 selective blocker is often used to manage these conditions by targeting the receptor specifically to reduce heart rate and blood pressure.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling (By similarity). Involved in the regulation of sleep/wake behaviors (PubMed : 31473062).

See full target information Adrb1

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:889656 PubMed35517499

2022

The Apoptotic Resistance of BRCA1-Deficient Ovarian Cancer Cells is Mediated by cAMP.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yue,Jihong Ma,Yinan Xiao,Pan Wang,Xiaoyang Gu,Bingteng Xie,Mo Li

Metabolism: clinical and experimental 116:154463 PubMed33309713

2020

Inhibiting glycolysis rescues memory impairment in an intellectual disability Gdi1-null mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Patrizia D'Adamo,Anemari Horvat,Antonia Gurgone,Maria Lidia Mignogna,Veronica Bianchi,Michela Masetti,Maddalena Ripamonti,Stefano Taverna,Jelena Velebit,Maja Malnar,Marko Muhič,Katja Fink,Angela Bachi,Umberto Restuccia,Sara Belloli,Rosa Maria Moresco,Alessia Mercalli,Lorenzo Piemonti,Maja Potokar,Saša Trkov Bobnar,Marko Kreft,Helena H Chowdhury,Matjaž Stenovec,Nina Vardjan,Robert Zorec

The Journal of clinical investigation 124:2099-112 PubMed24713652

2014

Vascular rarefaction mediates whitening of brown fat in obesity.

Applications

Species

Mouse

Ippei Shimizu,Tamar Aprahamian,Ryosuke Kikuchi,Ayako Shimizu,Kyriakos N Papanicolaou,Susan MacLauchlan,Sonomi Maruyama,Kenneth Walsh

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 28:440-52 PubMed24100020

2013

Antiobese function of platelet-activating factor: increased adiposity in platelet-activating factor receptor-deficient mice with age.

Applications

Unspecified application

Species

Unspecified reactive species

Junko Sugatani,Satoshi Sadamitsu,Masahiko Yamaguchi,Yasuhiro Yamazaki,Ryoko Higa,Yoshiki Hattori,Takahiro Uchida,Akira Ikari,Wataru Sugiyama,Tatsuo Watanabe,Satoshi Ishii,Masao Miwa,Takao Shimizu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta 1 Adrenergic Receptor antibody

Immunocytochemistry/ Immunofluorescence - Anti-beta 1 Adrenergic Receptor antibody (AB85037)

Western blot - Anti-beta 1 Adrenergic Receptor antibody (AB85037)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise