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AB129065

Anti-beta 1 Spectrin antibody [EPR6259]

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(3 Publications)

Rabbit Recombinant Monoclonal beta 1 Spectrin antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

SPTB1, SPTB, Beta-I spectrin

1 Images
Western blot - Anti-beta 1 Spectrin antibody [EPR6259] (AB129065)
  • WB

Unknown

Western blot - Anti-beta 1 Spectrin antibody [EPR6259] (AB129065)

All lanes:

Western blot - Anti-beta 1 Spectrin antibody [EPR6259] (ab129065) at 1/1000 dilution

All lanes:

Red blood cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 23 kDa,246 kDa

false

  • Carrier free

    Anti-beta 1 Spectrin antibody [EPR6259] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6259

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta 1 Spectrin also known as SPTB is a large cytoskeletal protein with a mass of approximately 247 kDa. It mechanically contributes to the stabilization of the plasma membrane by forming a meshwork beneath it. This protein links the plasma membrane to the actin cytoskeleton. SPTB is abundantly expressed in erythrocytes and neurons where it plays important roles in maintaining cell shape and structural integrity.
Biological function summary

Beta 1 Spectrin is part of the spectrin-actin cytoskeleton complex and participates in cellular functions such as maintaining membrane integrity and flexibility. It anchors various proteins to the cytoskeleton and regulates membrane protein distribution. The interactions of beta 1 Spectrin within cells ensure proper localization of membrane channels and receptors critical for cellular signaling and transport activities.

Pathways

Beta 1 Spectrin functions in pathways connected to membrane cytoskeleton dynamics and cellular environmental responses. It closely relates to the ankyrin pathway where it interacts with ankyrin proteins to connect spectrin-actin network to plasma membrane proteins. Furthermore beta 1 Spectrin is involved in the maintenance and organization of lipid rafts which are specialized membrane microdomains enriched with specific lipids and proteins.

Beta 1 Spectrin mutations and defects associate with conditions like hereditary spherocytosis and spinocerebellar ataxia. In hereditary spherocytosis defects in beta 1 Spectrin lead to hemolytic anemia due to the altered erythrocyte membrane stability. This protein also interacts with ankyrin and band 3 proteins which are important in red blood cell membrane architecture and function. In spinocerebellar ataxia abnormalities in beta 1 Spectrin affect neuronal function contributing to progressive motor coordination issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
See full target information SPTB
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Annals of human genetics 89:450-458 PubMed40551579

2025

Novel SPTB Variations Cause Hereditary Spherocytosis With Cholangiolithiasis and Severe Intrahepatic Cholestasis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Lin Li,Sadik Ali,Qiong Bin

Journal of cellular and molecular medicine 23:4454-4463 PubMed31016877

2019

Two novel ANK1 loss-of-function mutations in Chinese families with hereditary spherocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Hao,Shanshan Li,Duan Ma,Shiyu Chen,Bowen Zhang,Deyong Xiao,Jin Zhang,Nan Jiang,Shayi Jiang,Jing Ma

Antioxidants (Basel, Switzerland) 6: PubMed28704937

2017

Attenuation of Red Blood Cell Storage Lesions with Vitamin C.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberly Sanford,Bernard J Fisher,Evan Fowler,Alpha A Fowler,Ramesh Natarajan
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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