Rabbit Recombinant Monoclonal beta 1 Spectrin antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-beta 1 Spectrin antibody [EPR6259] - BSA and Azide free (AB248275), expandable thumbnail](https://content.abcam.com/products/images/beta-1-spectrin-antibody-epr6259-bsa-and-azide-free-ab248275--western-blot-img136522.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta 1 Spectrin antibody [EPR6259] - BSA and Azide free (AB248275)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Tested | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
Alternative names
SPTB1, SPTB, Beta-I spectrin
Recommended products
Rabbit Recombinant Monoclonal beta 1 Spectrin antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6259
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248275 is the carrier-free version of Anti-beta 1 Spectrin antibody [EPR6259] ab129065.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta 1 Spectrin also known as SPTB is a large cytoskeletal protein with a mass of approximately 247 kDa. It mechanically contributes to the stabilization of the plasma membrane by forming a meshwork beneath it. This protein links the plasma membrane to the actin cytoskeleton. SPTB is abundantly expressed in erythrocytes and neurons where it plays important roles in maintaining cell shape and structural integrity.
- Biological function summary
Beta 1 Spectrin is part of the spectrin-actin cytoskeleton complex and participates in cellular functions such as maintaining membrane integrity and flexibility. It anchors various proteins to the cytoskeleton and regulates membrane protein distribution. The interactions of beta 1 Spectrin within cells ensure proper localization of membrane channels and receptors critical for cellular signaling and transport activities.
- Pathways
Beta 1 Spectrin functions in pathways connected to membrane cytoskeleton dynamics and cellular environmental responses. It closely relates to the ankyrin pathway where it interacts with ankyrin proteins to connect spectrin-actin network to plasma membrane proteins. Furthermore beta 1 Spectrin is involved in the maintenance and organization of lipid rafts which are specialized membrane microdomains enriched with specific lipids and proteins.
- Associated diseases and disorders
Beta 1 Spectrin mutations and defects associate with conditions like hereditary spherocytosis and spinocerebellar ataxia. In hereditary spherocytosis defects in beta 1 Spectrin lead to hemolytic anemia due to the altered erythrocyte membrane stability. This protein also interacts with ankyrin and band 3 proteins which are important in red blood cell membrane architecture and function. In spinocerebellar ataxia abnormalities in beta 1 Spectrin affect neuronal function contributing to progressive motor coordination issues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-beta 1 Spectrin antibody [EPR6259] - BSA and Azide free (ab248275), expandable thumbnail](https://content.abcam.com/products/images/beta-1-spectrin-antibody-epr6259-bsa-and-azide-free-ab248275--western-blot-img136522.jpg "Western blot - Anti-beta 1 Spectrin antibody [EPR6259] - BSA and Azide free (ab248275)")
Western blot - Anti-beta 1 Spectrin antibody [EPR6259] - BSA and Azide free (ab248275)
This data was developed using Anti-beta 1 Spectrin antibody [EPR6259] ab129065, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-beta 1 Spectrin antibody [EPR6259] (Anti-beta 1 Spectrin antibody [EPR6259] ab129065) at 1/1000 dilution
All lanes: Red blood cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 23 kDa, 246 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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