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AB248275

Anti-beta 1 Spectrin antibody [EPR6259] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal beta 1 Spectrin antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SPTB1, SPTB, Beta-I spectrin

1 Images
Western blot - Anti-beta 1 Spectrin antibody [EPR6259] - BSA and Azide free (AB248275)
  • WB

Unknown

Western blot - Anti-beta 1 Spectrin antibody [EPR6259] - BSA and Azide free (AB248275)

This data was developed using ab129065, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-beta 1 Spectrin antibody [EPR6259] (<a href='/en-us/products/primary-antibodies/beta-1-spectrin-antibody-epr6259-ab129065'>ab129065</a>) at 1/1000 dilution

All lanes:

Red blood cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 23 kDa,246 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6259

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab248275 is the carrier-free version of ab129065.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta 1 Spectrin also known as SPTB is a large cytoskeletal protein with a mass of approximately 247 kDa. It mechanically contributes to the stabilization of the plasma membrane by forming a meshwork beneath it. This protein links the plasma membrane to the actin cytoskeleton. SPTB is abundantly expressed in erythrocytes and neurons where it plays important roles in maintaining cell shape and structural integrity.
Biological function summary

Beta 1 Spectrin is part of the spectrin-actin cytoskeleton complex and participates in cellular functions such as maintaining membrane integrity and flexibility. It anchors various proteins to the cytoskeleton and regulates membrane protein distribution. The interactions of beta 1 Spectrin within cells ensure proper localization of membrane channels and receptors critical for cellular signaling and transport activities.

Pathways

Beta 1 Spectrin functions in pathways connected to membrane cytoskeleton dynamics and cellular environmental responses. It closely relates to the ankyrin pathway where it interacts with ankyrin proteins to connect spectrin-actin network to plasma membrane proteins. Furthermore beta 1 Spectrin is involved in the maintenance and organization of lipid rafts which are specialized membrane microdomains enriched with specific lipids and proteins.

Beta 1 Spectrin mutations and defects associate with conditions like hereditary spherocytosis and spinocerebellar ataxia. In hereditary spherocytosis defects in beta 1 Spectrin lead to hemolytic anemia due to the altered erythrocyte membrane stability. This protein also interacts with ankyrin and band 3 proteins which are important in red blood cell membrane architecture and function. In spinocerebellar ataxia abnormalities in beta 1 Spectrin affect neuronal function contributing to progressive motor coordination issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
See full target information SPTB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 10:e13735 PubMed35891648

2022

Identification and validation of immune related core transcription factors in NAFLD.

Applications

Unspecified application

Species

Unspecified reactive species

Minbo Zhang,Yu Zhang,Xiaoxiao Jiao,Linying Lai,Yiting Qian,Bo Sun,Wenzhuo Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com