Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (AB182136)

IHC image of beta 2 Adrenergic Receptor staining in a section of frozen human prostate carcinoma performed on a Leica BOND^TM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab182136, 1/750 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (AB182136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1 : 100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (AB182136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1 : 100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (AB182136)

Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1/100 (1.3 μg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. DAPI was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (AB182136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling beta 2 Adrenergic Receptor with Purified ab182136 at 1 : 100 dilution (1.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

• WB

Unknown

Western blot - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (AB182136)

Blocking and diluting buffer : 5% NFDM/TBST

The 50-90kda bands detected by ab182136 are consistent with many literatures, like PMID : 2836733, PMID : 17570158, PMID : 11701618, PMID : 2545714, PMID : 16708760.

These bands have not been confirmed experimentally.

All lanes:

Western blot - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136) at 0.1 µg/mL

Lane 1:

A431 ( Human epidermoid carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Human skeletal muscle lysates at 20 µg

Lane 3:

Human fetal liver lysates at 20 µg

Lane 4:

Mouse heart lysates at 20 µg

Lane 5:

Rat heart lysates at 20 µg

Lane 6:

Mouse kidney lysates at 20 µg

Lane 7:

Rat kidney lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

false

• WB

Lab

Western blot - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (AB182136)

The 50-90kda bands detected by ab182136 are consistent with many literatures, like PMID : 2836733, PMID : 17570158, PMID : 11701618, PMID : 2545714, PMID : 16708760.

These bands have not been confirmed experimentally.

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (ab182136) at 1/1000 dilution

Lane 1:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Human skeletal muscle lysate at 20 µg

Lane 3:

Human fetal liver lysate at 20 µg

Lane 4:

Mouse heart lysate at 20 µg

Lane 5:

Rat heart lysate at 20 µg at 20 µg

Lane 6:

Mouse kidney lysate at 20 µg

Lane 7:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 50-90 kDa

false

Exposure time: 180s