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AB9871

Anti-beta 2 Defensin/BD-2 antibody

4

(2 Reviews)

|

(10 Publications)

Goat Polyclonal DFB4A antibody. Suitable for WB, sELISA and reacts with Human, Recombinant full length protein - Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DEFB4A.

View Alternative Names

DEFB102, DEFB2, DEFB4, DEFB4B, DEFB4A, Defensin beta 4A, Beta-defensin 2, Skin-antimicrobial peptide 1, BD-2, hBD-2, SAP1

1 Images
Sandwich ELISA - Anti-beta 2 Defensin/BD-2 antibody (AB9871)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-beta 2 Defensin/BD-2 antibody (AB9871)

Sandwich ELISA : To detect Human BD-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of beta-2 Defensin Polyclonal Antibody is required. This antigen affinity purified antibody, in conjunction with a beta-2 Defensin Polyclonal Antibody, Biotin as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-2.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

sELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human DEFB4A.

O15263

Reactivity data

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Product details

hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.

Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in water
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta 2 defensin also known as BD-2 or beta-defensin 2 is a small antimicrobial peptide with a molecular mass of about 4.6 kDa. It plays an important role in the innate immune response by disrupting microbial membranes. BD-2 is expressed in epithelial cells making it important for creating a protective barrier in the skin respiratory tract and urogenital tract. This peptide acts directly against bacteria fungi and some viruses helping to maintain microbial homeostasis in these areas.
Biological function summary

Beta 2 defensin serves as a critical component of host defense mechanisms. It not only shows antimicrobial properties but also influences inflammatory processes. BD-2 interacts with immune cells recruiting and activating them for enhanced defense response. Presently there is no evidence of beta 2 defensin being part of a larger protein complex focusing its function on immediate immune response and cellular signaling in areas it is expressed.

Pathways

Beta 2 defensin engages in critical pathways of immune protection. It is part of the toll-like receptor signaling pathway and engages the NF-kB pathway. These pathways are essential for activating immune responses when pathogens are recognized. Additionally beta 2 defensin interacts with other defensins and related antimicrobial peptides to mount an effective immune response coordinating with various cytokines and chemokines to balance protective actions.

Beta 2 defensin has connections to inflammatory and autoimmune conditions. It has a potential link to psoriasis where its expression levels are increased serving both protective and inflammatory roles. BD-2 is also investigated in cystic fibrosis where its impaired function can contribute to chronic lung infections. In these conditions the altered balance of BD-2 associates it with proteins involved in immune dysregulation contributing to the pathophysiology of these disorders.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria (PubMed : 10837369, PubMed : 9202117). Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM (PubMed : 10837369). Also exhibits antimicrobial activity against the yeast C.albicans (PubMed : 10837369, PubMed : 30050988, PubMed : 9202117). Permeabilizes C.albicans cell membranes via targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), thereby leading to cell fragmentation and cell death (PubMed : 30050988). Acts as a ligand for C-C chemokine receptor CCR6 (PubMed : 10521347, PubMed : 20068036). Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells, such as immature dendritic cells and memory T cells (PubMed : 10521347, PubMed : 20068036).
See full target information DEFB4A

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Microbiome 11:227 PubMed37849006

2023

Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models.

Applications

Unspecified application

Species

Unspecified reactive species

Gijs Rikken,Luca D Meesters,Patrick A M Jansen,Diana Rodijk-Olthuis,Ivonne M J J van Vlijmen-Willems,Hanna Niehues,Jos P H Smits,Peter Oláh,Bernhard Homey,Joost Schalkwijk,Patrick L J M Zeeuwen,Ellen H van den Bogaard

iScience 26:106483 PubMed37096035

2023

Gram-positive anaerobic cocci guard skin homeostasis by regulating host-defense mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Danique A van der Krieken,Gijs Rikken,Thomas H A Ederveen,Patrick A M Jansen,Diana Rodijk-Olthuis,Luca D Meesters,Ivonne M J J van Vlijmen-Willems,Bram van Cranenbroek,Renate G van der Molen,Joost Schalkwijk,Ellen H van den Bogaard,Patrick L J M Zeeuwen

Frontiers in veterinary science 9:925701 PubMed35812842

2022

Activated Mesenchymal Stromal Cell Therapy for Treatment of Multi-Drug Resistant Bacterial Infections in Dogs.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie Johnson,Lyndah Chow,Jacqueline Harrison,Sirikul Soontararak,Steven Dow

Stem cells translational medicine 9:235-249 PubMed31702119

2019

Antibacterial activity of human mesenchymal stem cells mediated directly by constitutively secreted factors and indirectly by activation of innate immune effector cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lyndah Chow,Valerie Johnson,Renata Impastato,Jonathan Coy,Alyssa Strumpf,Steven Dow

The Journal of investigative dermatology 140:415-424.e10 PubMed31344386

2019

Targeting the Cutaneous Microbiota in Atopic Dermatitis by Coal Tar via AHR-Dependent Induction of Antimicrobial Peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Jos P H Smits,Thomas H A Ederveen,Gijs Rikken,Noa J M van den Brink,Ivonne M J J van Vlijmen-Willems,Jos Boekhorst,Marijke Kamsteeg,Joost Schalkwijk,Sacha A F T van Hijum,Patrick L J M Zeeuwen,Ellen H van den Bogaard

Scientific reports 8:10044 PubMed29968819

2018

Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Sija Landman,Vivian L de Oliveira,Piet E J van Erp,Esther Fasse,Stijn C G Bauland,Irma Joosten,Hans J P M Koenen

The Journal of investigative dermatology 130:2611-20 PubMed20631729

2010

A comprehensive analysis of pattern recognition receptors in normal and inflamed human epidermis: upregulation of dectin-1 in psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Heleen D de Koning,Diana Rodijk-Olthuis,Ivonne M J J van Vlijmen-Willems,Leo A B Joosten,Mihai G Netea,Joost Schalkwijk,Patrick L J M Zeeuwen

Respiratory medicine 103:201-8 PubMed18980836

2008

Tracheostomy and related host-pathogen interaction are associated with airway inflammation as characterized by tracheal aspirate analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Patrizia Pignatti,Antonella Balestrino,Christian Herr,Robert Bals,Dania Moretto,Massimo Corradi,Rossella Alinovi,Monica Delmastro,Claus Vogelmeier,Stefano Nava,Gianna Moscato,Bruno Balbi

Thorax 64:144-9 PubMed18852155

2008

Suppression of pulmonary innate host defence in smokers.

Applications

Unspecified application

Species

Unspecified reactive species

C Herr,C Beisswenger,C Hess,K Kandler,N Suttorp,T Welte,J-M Schroeder,C Vogelmeier

Clinical and experimental immunology 131:90-101 PubMed12519391

2003

Increased expression of antimicrobial peptides and lysozyme in colonic epithelial cells of patients with ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

A Fahlgren,S Hammarström,A Danielsson,M-L Hammarström
View all publications

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