Goat Polyclonal DFB4A antibody. Suitable for WB, sELISA, Neut and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DEFB4A.
IgG
Goat
pH: 7.2
Constituents: PBS
Lyophilized
Polyclonal
| WB | sELISA | Neut | |
|---|---|---|---|
| Human | Expected | Expected | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes To detect beta 2 Defensin/hBD-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant beta 2 Defensin/hBD-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions |
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes To detect beta 2 Defensin/hBD-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83509 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant beta 2 Defensin/hBD-2. |
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes To yield one-half maximal inhibition [ND50] of the biological activity of Human beta 2 Defensin/hBD-2 (1.0 ug/ml), a concentration of 5.0 - 8.0 ug/ml of this antibody is required. |
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Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Can bind to both human and mouse CCR6 and induce chemotactic activity of CCR6-expressing cells (PubMed:20068036).
Beta-defensin 4A, Beta-defensin 2, Skin-antimicrobial peptide 1, BD-2, hBD-2, SAP1, DEFB102, DEFB4A, DEFB2, DEFB4, DEFB4B
Goat Polyclonal DFB4A antibody. Suitable for WB, sELISA, Neut and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DEFB4A.
Beta-defensin 4A, Beta-defensin 2, Skin-antimicrobial peptide 1, BD-2, hBD-2, SAP1, DEFB102, DEFB4A, DEFB2, DEFB4, DEFB4B
IgG
Goat
pH: 7.2
Constituents: PBS
Lyophilized
Polyclonal
Affinity purification Immunogen
Centrifuge vial prior to opening. Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described, then no further reconstitution is necessary. The lyophilized antibody is stable for at least 2 years from date of receipt at -20C. The reconstituted antibody is stable for at least two weeks at 2-8C. Frozen aliquots are stable for at least 6 months when stored at -20C. Avoid repeated freeze-thaw cycles.
Blue Ice
+4°C
hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.
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This supplementary information is collated from multiple sources and compiled automatically.
Beta 2 defensin also known as BD-2 or beta-defensin 2 is a small antimicrobial peptide with a molecular mass of about 4.6 kDa. It plays an important role in the innate immune response by disrupting microbial membranes. BD-2 is expressed in epithelial cells making it important for creating a protective barrier in the skin respiratory tract and urogenital tract. This peptide acts directly against bacteria fungi and some viruses helping to maintain microbial homeostasis in these areas.
Beta 2 defensin serves as a critical component of host defense mechanisms. It not only shows antimicrobial properties but also influences inflammatory processes. BD-2 interacts with immune cells recruiting and activating them for enhanced defense response. Presently there is no evidence of beta 2 defensin being part of a larger protein complex focusing its function on immediate immune response and cellular signaling in areas it is expressed.
Beta 2 defensin engages in critical pathways of immune protection. It is part of the toll-like receptor signaling pathway and engages the NF-kB pathway. These pathways are essential for activating immune responses when pathogens are recognized. Additionally beta 2 defensin interacts with other defensins and related antimicrobial peptides to mount an effective immune response coordinating with various cytokines and chemokines to balance protective actions.
Beta 2 defensin has connections to inflammatory and autoimmune conditions. It has a potential link to psoriasis where its expression levels are increased serving both protective and inflammatory roles. BD-2 is also investigated in cystic fibrosis where its impaired function can contribute to chronic lung infections. In these conditions the altered balance of BD-2 associates it with proteins involved in immune dysregulation contributing to the pathophysiology of these disorders.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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