AB14426

Anti-beta 2 Defensin/BD-2 antibody [B 235-I]

Name: Anti-beta 2 Defensin/BD-2 antibody [B 235-I] (ab14426) | Abcam
Brand: Abcam Limited
SKU: AB14426

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(1 Publication)

Mouse Monoclonal DFB4A antibody. Suitable for ELISA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DEFB4A aa 1-50.

View Alternative Names

DEFB102, DEFB2, DEFB4, DEFB4B, DEFB4A, Defensin beta 4A, Beta-defensin 2, Skin-antimicrobial peptide 1, BD-2, hBD-2, SAP1

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B 235-I

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ELISA

applications

Immunogen

Synthetic Peptide within Human DEFB4A aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O15263

Specificity

ab14426 recognises synthetic beta 2 Defensin/BD-2.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein L

Purification notes

Cell culture supernatant, protein L purified.

Storage buffer

pH: 7.4 Constituents: 0.605% Tris

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta 2 defensin also known as BD-2 or beta-defensin 2 is a small antimicrobial peptide with a molecular mass of about 4.6 kDa. It plays an important role in the innate immune response by disrupting microbial membranes. BD-2 is expressed in epithelial cells making it important for creating a protective barrier in the skin respiratory tract and urogenital tract. This peptide acts directly against bacteria fungi and some viruses helping to maintain microbial homeostasis in these areas.

Biological function summary

Beta 2 defensin serves as a critical component of host defense mechanisms. It not only shows antimicrobial properties but also influences inflammatory processes. BD-2 interacts with immune cells recruiting and activating them for enhanced defense response. Presently there is no evidence of beta 2 defensin being part of a larger protein complex focusing its function on immediate immune response and cellular signaling in areas it is expressed.

Pathways

Beta 2 defensin engages in critical pathways of immune protection. It is part of the toll-like receptor signaling pathway and engages the NF-kB pathway. These pathways are essential for activating immune responses when pathogens are recognized. Additionally beta 2 defensin interacts with other defensins and related antimicrobial peptides to mount an effective immune response coordinating with various cytokines and chemokines to balance protective actions.

Beta 2 defensin has connections to inflammatory and autoimmune conditions. It has a potential link to psoriasis where its expression levels are increased serving both protective and inflammatory roles. BD-2 is also investigated in cystic fibrosis where its impaired function can contribute to chronic lung infections. In these conditions the altered balance of BD-2 associates it with proteins involved in immune dysregulation contributing to the pathophysiology of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria (PubMed : 10837369, PubMed : 9202117). Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM (PubMed : 10837369). Also exhibits antimicrobial activity against the yeast C.albicans (PubMed : 10837369, PubMed : 30050988, PubMed : 9202117). Permeabilizes C.albicans cell membranes via targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), thereby leading to cell fragmentation and cell death (PubMed : 30050988). Acts as a ligand for C-C chemokine receptor CCR6 (PubMed : 10521347, PubMed : 20068036). Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells, such as immature dendritic cells and memory T cells (PubMed : 10521347, PubMed : 20068036).

See full target information DEFB4A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 15:141-152 PubMed35035226

2022

Tumor-Infiltrating CD4 Central Memory T Cells Correlated with Favorable Prognosis in Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Wu,Tianyi Zhang,Haofeng Xiong,Liujun Zeng,Zijia Wang,Ying Peng,Weijun Chen,Xin Hu,Tong Su

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta 2 Defensin/BD-2 antibody [B 235-I]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise