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Mouse Monoclonal beta 2 Microglobulin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human B2M.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [3F9-2C2] (AB54810), expandable thumbnail
  • Western blot - Anti-beta 2 Microglobulin antibody [3F9-2C2] (AB54810), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.4

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human B2M. Database link P61769

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Associated Products

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Target data

Function

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).

Alternative names

Recommended products

Mouse Monoclonal beta 2 Microglobulin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human B2M.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human B2M. Database link P61769
Clone number

3F9-2C2

Purity

Tissue culture supernatant

Light chain type

kappa

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Associated diseases and disorders

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

Product promise

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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [3F9-2C2] (ab54810), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [3F9-2C2] (ab54810)

    beta 2 Microglobulin antibody (ab54810) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human esophagus.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-beta 2 Microglobulin antibody [3F9-2C2] (ab54810), expandable thumbnail

    Western blot - Anti-beta 2 Microglobulin antibody [3F9-2C2] (ab54810)

    beta 2 Microglobulin antibody (ab54810) at 1ug/lane + U-2 cell lysate at 25ug/lane.

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-beta 2 Microglobulin antibody [3F9-2C2] (ab54810)

    Predicted band size: 14 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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