AB175031

Anti-beta 2 Microglobulin antibody

Name: Anti-beta 2 Microglobulin antibody (ab175031) | Abcam
Brand: Abcam Limited
SKU: AB175031
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal beta 2 Microglobulin antibody. Suitable for WB, ICC/IF and reacts with African green monkey, Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human B2M.

View Alternative Names

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody (AB175031)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody (AB175031)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab175031. Blue DAPI for nuclear staining.

Supplier Data

Western blot - Anti-beta 2 Microglobulin antibody (AB175031)

All lanes:

Western blot - Anti-beta 2 Microglobulin antibody (ab175031) at 1/500 dilution

Lane 1:

Jurkat cell extract

Lane 2:

COS-7 cell extract

Lane 3:

HeLa cell extract

Lane 4:

K562 cell extract

Lane 5:

Mouse lung extract

Lane 6:

Mouse liver extract

Lane 7:

Mouse spleen extract

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, African green monkey

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human B2M.

P61769

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/499 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).

See full target information B2M

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 20: PubMed31091675

2019

Cadmium Complexed with β2-Microglubulin, Albumin and Lipocalin-2 rather than Metallothionein Cause Megalin:Cubilin Dependent Toxicity of the Renal Proximal Tubule.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Fels,Bettina Scharner,Ralf Zarbock,Itzel Pamela Zavala Guevara,Wing-Kee Lee,Olivier C Barbier,Frank Thévenod

Scientific reports 8:6205 PubMed29670130

2018

A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells associated with Enhanced Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Abhijit Basu,Saira Munir,Medanie A Mulaw,Karmveer Singh,Diana Crisan,Anca Sindrilaru,Nicolai Treiber,Meinhard Wlaschek,Markus Huber-Lang,Florian Gebhard,Karin Scharffetter-Kochanek

PloS one 10:e0124420 PubMed25933295

2015

HGF Gene Modification in Mesenchymal Stem Cells Reduces Radiation-Induced Intestinal Injury by Modulating Immunity.

Applications

IHC

Species

Unspecified reactive species

Hua Wang,Rui-Ting Sun,Yang Li,Yue-Feng Yang,Feng-Jun Xiao,Yi-Kun Zhang,Shao-Xia Wang,Hui-Yan Sun,Qun-Wei Zhang,Chu-Tse Wu,Li-Sheng Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta 2 Microglobulin antibody

Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody (AB175031)

Western blot - Anti-beta 2 Microglobulin antibody (AB175031)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise