Skip to main content

Rabbit Polyclonal beta 2 Microglobulin antibody. Suitable for WB and reacts with Human, Dog samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Dog B2M.

Be the first to review this product! Submit a review

Images

Western blot - Anti-beta 2 Microglobulin antibody (AB231225), expandable thumbnail
  • Western blot - Anti-beta 2 Microglobulin antibody (AB231225), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Dog B2M. Database link P19341

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Dog
Tested

Tested
Tested

Species

Human

Dilution info
0.5-2 µg/mL
Notes

-

Species

Dog

Dilution info
0.5-2 µg/mL
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).

Alternative names

Recommended products

Rabbit Polyclonal beta 2 Microglobulin antibody. Suitable for WB and reacts with Human, Dog samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Dog B2M.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Dog B2M. Database link P19341
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Associated diseases and disorders

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-beta 2 Microglobulin antibody (ab231225), expandable thumbnail

    Western blot - Anti-beta 2 Microglobulin antibody (ab231225)

    All lanes: Western blot - Anti-beta 2 Microglobulin antibody (ab231225) at 2 µg/mL

    All lanes: Human urine

    Secondary

    All lanes: HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

    Predicted band size: 14 kDa

  • Western blot - Anti-beta 2 Microglobulin antibody (ab231225), expandable thumbnail

    Western blot - Anti-beta 2 Microglobulin antibody (ab231225)

    All lanes: Western blot - Anti-beta 2 Microglobulin antibody (ab231225) at 2 µg/mL

    All lanes: Recombinant dog beta 2 microglobulin

    Secondary

    All lanes: HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

    Predicted band size: 14 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com