AB6608

Anti-beta 2 Microglobulin antibody

Name: Anti-beta 2 Microglobulin antibody (ab6608) | Abcam
Brand: Abcam Limited
SKU: AB6608
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal beta 2 Microglobulin antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human B2M.

View Alternative Names

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human B2M.

P61769

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "" } } }

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Product details

The antibody reacts with b2-microglobulin isolated from human urine showing a 12.8 kDa band under non-reducing condition of SDS-PAGE.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification technique

Ion exchange chromatography

Purification notes

Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).

See full target information B2M

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Immunity 54:1772-1787.e9 PubMed34289378

2021

β-microglobulin triggers NLRP3 inflammasome activation in tumor-associated macrophages to promote multiple myeloma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Hofbauer,Dimitrios Mougiakakos,Luca Broggini,Mario Zaiss,Maike Büttner-Herold,Christian Bach,Bernd Spriewald,Frank Neumann,Savita Bisht,Jens Nolting,Robert Zeiser,Shaima'a Hamarsheh,Martin Eberhardt,Julio Vera,Cristina Visentin,Chiara Maria Giulia De Luca,Fabio Moda,Stefan Haskamp,Cindy Flamann,Martin Böttcher,Katrin Bitterer,Simon Völkl,Andreas Mackensen,Stefano Ricagno,Heiko Bruns

Antioxidants & redox signaling 15:635-44 PubMed21050141

2011

Dimerization of soluble disulfide trap single-chain major histocompatibility complex class I molecules dependent on peptide binding affinity.

Applications

Species

Human

Eleni Kotsiou,Joanna Brzostek,Izabela Lenart,Antony N Antoniou,Julian Dyson,Keith G Gould

Hepatology (Baltimore, Md.) 51:654-9 PubMed20017200

2009

Huh-7: a human "hemochromatotic" cell line.

Applications

ICC/IF

Species

Human

Chiara Vecchi,Giuliana Montosi,Antonello Pietrangelo

PloS one 3:e3858 PubMed19050760

2008

GPRC6A null mice exhibit osteopenia, feminization and metabolic syndrome.

Applications

Species

Human

Min Pi,Ling Chen,Min-Zhao Huang,Wenyu Zhu,Brian Ringhofer,Junming Luo,Lane Christenson,Benyi Li,Jianghong Zhang,P David Jackson,Pieter Faber,Kurt R Brunden,John J Harrington,L Darryl Quarles

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta 2 Microglobulin antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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