AB759

Anti-beta 2 Microglobulin antibody [B2M-01]

Name: Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) | Abcam
Brand: Abcam Limited
SKU: AB759
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(13 Publications)

Mouse Monoclonal beta 2 Microglobulin antibody. Suitable for WB, Flow Cyt and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human B2M.

View Alternative Names

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

3 Images

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M-01] (AB759)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M-01] (AB759)

Flow cytometric analysis of Human Peripheral Blood cells labelling beta 2 Microglobulin with ab759 at 0.3 ug/ml showing separation of human monocytes (red-filled) from human beta2-Microglobulin negative blood debris (black-dashed).

Supplier Data

Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (AB759)

All lanes:

Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) at 2 µg/mL

Lane 1:

Raji cell lysate under non-reducing condition

Lane 2:

Jurkat cell lysate under non-reducing condition

Lane 3:

HeLa cell lysate under non-reducing condition

Lane 4:

Daudi cell lysate under non-reducing condition

Lane 5:

Raji cell lysate under reducing condition

Lane 6:

Jurkat cell lysate under reducing condition

Lane 7:

HeLa cell lysate under reducing condition

Lane 8:

Daudi cell lysate under reducing condition

Secondary

All lanes:

IRDye800-conjugated anti-mouse secondary antibody

Predicted band size: 14 kDa

Observed band size: 12 kDa

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Unknown

Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (AB759)

Lysates were separated on 15% SDS–PAGE gel and electrotransferred to a PVDF membrane. The membrane was blocked in PBS/Tween buffer containing 5% non fat dry milk for 2 hours and probed with ab759 at 4°C overnight.

All lanes:

Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) at 2 µg/mL

Lane 1:

JEG-3 cell lysate

Lane 2:

A-431 cell lysate

Lane 3:

U-937 cell lysate

Lane 4:

Raji cell lysate

Predicted band size: 14 kDa

true

Biotin

Biotin Anti-beta 2 Microglobulin antibody [B2M-01]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-beta 2 Microglobulin antibody [B2M-01]
578 PE

PE Anti-beta 2 Microglobulin antibody [B2M-01]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B2M-01

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Native Full Length Protein corresponding to Human B2M.

P61769

Specificity

This antibody reacts with beta-2-microglobulin, associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble forms of beta-2-microglobulin. The antibody detected beta-2-microglobulin in U-937 and RAJI cell lines in WB in non reducing conditions however failed to detect a band in JEG-3 and A431 cell lysates. All lysates gave a signal in direct ELISA.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5-4 µg/mL", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Chicken": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Cow": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Dog": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Pig": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rabbit": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Purified by precipitation methods. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).

See full target information B2M

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials research 29:0152 PubMed39990979

2025

Insulin-Like Growth Factor 2 Secreted from Mesenchymal Stem Cells with High Glutathione Levels Alleviates Osteoarthritis via Paracrine Rejuvenation of Senescent Chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Gun Hee Cho,Hyun Cheol Bae,Yu Jeong Lee,Ha Ru Yang,Hyewon Kang,Hee Jung Park,Sun Young Wang,You Jung Kim,Heun-Soo Kang,In Gyu Kim,Byung Sun Choi,Hyuk-Soo Han

Journal of immunology research 2017:8959687 PubMed29318161

2017

Activation of Peptidylarginine Deiminase in the Salivary Glands of Mice Drives the Citrullination of Ro and La Ribonucleoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

Mayra Rodríguez-Rodríguez,Rafael Herrera-Esparza,Juan-José Bollain Y Goytia,María-Elena Pérez-Pérez,Deyanira Pacheco-Tovar,Jessica Murillo-Vázquez,Guadalupe Pacheco-Tovar,Esperanza Avalos-Díaz

BMC neuroscience 16:24 PubMed25896789

2015

Expression and cellular localization of hepcidin mRNA and protein in normal rat brain.

Applications

Species

Unspecified reactive species

Ruma Raha-Chowdhury,Animesh Alexander Raha,Serhiy Forostyak,Jing-Wei Zhao,Simon Russell William Stott,Adrian Bomford

Autoimmune diseases 2013:548064 PubMed23533719

2013

Posttranslational Protein Modification in the Salivary Glands of Sjögren's Syndrome Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael Herrera-Esparza,Mayra Rodríguez-Rodríguez,María Elena Pérez-Pérez,Martha Adriana Badillo-Soto,Felipe Torres-Del-Muro,Juan José Bollain-Y-Goytia,Deyanira Pacheco-Tovar,Esperanza Avalos-Díaz

The Journal of biological chemistry 288:13325-36 PubMed23519467

2013

Dual mechanism of integrin αIIbβ3 closure in procoagulant platelets.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine J A Mattheij,Karen Gilio,Roger van Kruchten,Shawn M Jobe,Adam J Wieschhaus,Athar H Chishti,Peter Collins,Johan W M Heemskerk,Judith M E M Cosemans

Journal of cell science 124:1672-80 PubMed21511727

2011

Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Shweta Tiwari,Janet A Askari,Martin J Humphries,Neil J Bulleid

Journal of proteome research 9:6-21 PubMed19678649

2009

Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B.

Applications

Species

Human

Astrid Slany,Verena J Haudek,Hannes Zwickl,Nina C Gundacker,Michael Grusch,Thomas S Weiss,Katja Seir,Chantal Rodgarkia-Dara,Claus Hellerbrand,Christopher Gerner

Journal of virology 83:3298-311 PubMed19158240

2009

Oncostatin M enhances the antiviral effects of type I interferon and activates immunostimulatory functions in liver epithelial cells.

Applications

Species

Human

Esther Larrea,Rafael Aldabe,Iranzu Gonzalez,Victor Segura,Pablo Sarobe,Itziar Echeverria,Jesus Prieto

Circulation 116:1396-403 PubMed17724262

2007

Beta2-microglobulin as a biomarker in peripheral arterial disease: proteomic profiling and clinical studies.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew M Wilson,Eiichiro Kimura,Randall K Harada,Nandini Nair,Balasubramanian Narasimhan,Xiao-Ying Meng,Fujun Zhang,Kendall R Beck,Jeffrey W Olin,Eric T Fung,John P Cooke

Oncogene 25:7650-61 PubMed17043663

2006

Proteomic identification of the wt-p53-regulated tumor cell secretome.

Applications

Unspecified application

Species

Unspecified reactive species

F W Khwaja,P Svoboda,M Reed,J Pohl,B Pyrzynska,E G Van Meir

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta 2 Microglobulin antibody [B2M-01]

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M-01] (AB759)

Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (AB759)

Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (AB759)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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