Mouse Monoclonal beta 2 Microglobulin antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human B2M.
IgG1
Mouse
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | Flow Cyt | WB | |
---|---|---|---|
Human | Tested | Tested | Tested |
Primates | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes (for 30 minutes at RT) Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Primates | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Primates | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Primates | Dilution info - | Notes - |
Select an associated product type
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).
CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin
Mouse Monoclonal beta 2 Microglobulin antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human B2M.
IgG1
Mouse
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
B2M/1118
Affinity purification Protein G
kappa
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
ab212752 is a carrier free version of Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882.
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Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.
Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.
Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.
Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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This data was developed using Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882, the same antibody clone in a different buffer formulation.
Flow Cytometric Analysis of HeLa cells labeling beta 2 Microglobulin with Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882; followed by goat anti-mouse IgG-CF488 (Blue); Unstained cells Control (Red).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882 at 1µg/ml.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882)
.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882).
All lanes: Western blot - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752) at 2 µg/mL
Lane 1: THP-1 cell lysate
Lane 2: Raji cell lysate
Predicted band size: 14 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrial carcinoma tissue labeling beta 2 Microglobulin with Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882 at 1µg/ml.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882)
.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882 at 1µg/ml.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882)
.
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