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Mouse Monoclonal beta 2 Microglobulin antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human B2M.

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Images

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (AB212752), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (AB212752), expandable thumbnail
  • Western blot - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (AB212752), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (AB212752), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (AB212752), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human B2M. Database link P61769

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Tested
Tested
Primates
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1.00000-2.00000 µg/mL

Notes

(for 30 minutes at RT)

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Primates

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1.00000-2.00000 µg for 106 Cells

Notes

-

Predicted
Predicted

Species

Primates

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1.00000-2.00000 µg/mL

Notes

-

Predicted
Predicted

Species

Primates

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

1 product for Alternative Version

Target data

Function

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).

Alternative names

Recommended products

Mouse Monoclonal beta 2 Microglobulin antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human B2M.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human B2M. Database link P61769
Carrier free

Yes

Clone number

B2M/1118

Purification technique

Affinity purification Protein G

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

ab212752 is a carrier free version of Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Associated diseases and disorders

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752), expandable thumbnail

    Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752)

    This data was developed using Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882, the same antibody clone in a different buffer formulation.

    Flow Cytometric Analysis of HeLa cells labeling beta 2 Microglobulin with Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882; followed by goat anti-mouse IgG-CF488 (Blue); Unstained cells Control (Red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882 at 1µg/ml.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882)

    .

  • Western blot - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752), expandable thumbnail

    Western blot - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882).

    All lanes: Western blot - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752) at 2 µg/mL

    Lane 1: THP-1 cell lysate

    Lane 2: Raji cell lysate

    Predicted band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrial carcinoma tissue labeling beta 2 Microglobulin with Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882 at 1µg/ml.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882)

    .

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/1118] - BSA and Azide free (ab212752)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882 at 1µg/ml.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [B2M/1118] ab215882)

    .

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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