AB215889

Anti-beta 2 Microglobulin antibody [B2M/961]

Name: Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889) | Abcam
Brand: Abcam Limited
SKU: AB215889
Price: 220 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal beta 2 Microglobulin antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human B2M.

View Alternative Names

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

8 Images

Supplier Data

Immunocytochemistry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunocytochemistry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling beta 2 Microglobulin with ab215889 at 4 μg/mL followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling beta 2 Microglobulin with ab215889 at 2 μg/10⁶ cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrial carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bladder carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

Supplier Data

Western blot - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

All lanes:

Western blot - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889) at 2 µg/mL

Lane 1:

THP-1 (human monocytic leukemia cell line) whole cell lysate

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate

Predicted band size: 14 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B2M/961

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human B2M.

P61769

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-4 µg/mL", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "Primary incubation for 30 minutes at room temperature." }, "Primates": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab215889 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).

See full target information B2M

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 8: PubMed36946463

2023

JAK-STAT activation contributes to cytotoxic T cell-mediated basal cell death in human chronic lung allograft dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Aaditya Khatri,Jamie L Todd,Fran L Kelly,Andrew Nagler,Zhicheng Ji,Vaibhav Jain,Simon G Gregory,Kent J Weinhold,Scott M Palmer

Nature communications 9:3634 PubMed30194383

2018

Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel B Ferguson,Ben Van Handel,Maxwell Bay,Petko Fiziev,Tonis Org,Siyoung Lee,Ruzanna Shkhyan,Nicholas W Banks,Mila Scheinberg,Ling Wu,Biagio Saitta,Joseph Elphingstone,A Noelle Larson,Scott M Riester,April D Pyle,Nicholas M Bernthal,Hanna Ka Mikkola,Jason Ernst,Andre J van Wijnen,Michael Bonaguidi,Denis Evseenko

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-beta 2 Microglobulin antibody [B2M/961]

Immunocytochemistry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Western blot - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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