Knockout Tested Rabbit Recombinant Monoclonal beta 2 Microglobulin antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.
Images
![Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (AB214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--western-blot-img144889.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (AB214769)")
![Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (AB214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--western-blot-img170663.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (AB214769)")
![Immunoprecipitation - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (AB214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--immunoprecipitation-img11142.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (AB214769)")
![Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (AB214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--flow-cytometry-intracellular-img25791.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (AB214769)")
![Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (AB214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--immunocytochemistry-immunofluorescence-img126657.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (AB214769)")
Publications
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- Oncotarget 6:11959-782015Systemic attenuation of the TGF-β pathway by a single drug simultaneously rejuvenates hippocampal neurogenesis and myogenesis in the same old mammal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanadie Yousef,Michael J Conboy,Adam Morgenthaler,Christina Schlesinger,Lukasz Bugaj,Preeti Paliwal,Christopher Greer,Irina M Conboy,David SchafferPubMed 26003168
- Journal of translational medicine 12:1662014Capillary nano-immunoassay for Akt 1/2/3 and 4EBP1 phosphorylation in acute myeloid leukemia.
- The Journal of physiology 591:4777-912013Neuronal major histocompatibility complex class I molecules are implicated in the generation of asymmetries in hippocampal circuitry.
- The Journal of experimental medicine 209:1363-772012VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Expected | Tested | Not recommended | Expected | Tested |
| Rat | Expected | Tested | Not recommended | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
5 products for Alternative Version
Target data
Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).
Alternative names
Beta-2-microglobulin, CDABP0092, B2M, HDCMA22P
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal beta 2 Microglobulin antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 4 publications.
Alternative names
Beta-2-microglobulin, CDABP0092, B2M, HDCMA22P
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP2978Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab214769 is the carrier-free version of Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.
- Biological function summary
Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.
- Pathways
Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.
- Associated diseases and disorders
Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--western-blot-img144889.jpg "Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)")
Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)
This data was developed using the same antibody clone in a different buffer formulation (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853).
Lanes 1-4: Merged signal (red and green). Green - Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 observed at 14 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 Anti-beta 2 Microglobulin antibody [EP2978Y] was shown to specifically react with beta 2 Microglobulin in wild-type HepG2 cells. Loss of signal was observed when knockout cell line Human B2M (beta 2 Microglobulin) knockout Hep G2 cell line ab262325 (knockout cell lysate Human B2M (beta 2 Microglobulin) knockout Hep G2 cell lysate ab256846) was used. Wild-type and beta 2 Microglobulin knockout samples were subjected to SDS-PAGE. Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853) at 1/1000 dilution
Lane 1: Wild-type HepG2 cell lysate at 20 µg
Lane 2: B2M knockout HepG2 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
![Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--western-blot-img170663.jpg "Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)")
Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)
False colour image of Western blot: Anti-beta 2 Microglobulin antibody [EP2978Y] staining at 1/5000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 was shown to bind specifically to beta 2 Microglobulin. A band was observed at 12 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in B2M knockout cell line Human B2M (beta 2 Microglobulin) knockout HEK-293T cell line ab266828 (knockout cell lysate Human B2M (beta 2 Microglobulin) knockout HEK-293T cell lysate ab256845). To generate this image, wild-type and B2M knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853) at 1/5000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: B2M knockout HEK-293T cell lysate at 20 µg
Lane 3: Wild-type A431 cell lysate at 20 µg
Lane 4: B2M knockout A431 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 12 kDa
![Immunoprecipitation - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--immunoprecipitation-img11142.jpg "Immunoprecipitation - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)")
Immunoprecipitation - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)
Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 (purified) at 1/20 immunoprecipitating beta 2 Microglobulin in Raji whole cell lysate.
Lane 1 (input): Raji whole cell lysate (10µg)
Lane 2 (+): Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 + Raji whole cell lysate (10µg).
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 in Raji whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853).
All lanes: Immunoprecipitation - Anti-beta 2 Microglobulin antibody [EP2978Y] (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853)
Predicted band size: 14 kDa
Observed band size: 14 kDa
![Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--flow-cytometry-intracellular-img25791.jpg "Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)")
Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling beta 2 Microglobulin with purified Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853).
![Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--immunocytochemistry-immunofluorescence-img126657.jpg "Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)")
Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling beta 2 Microglobulin with purified Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853).
![Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--western-blot-img432517.jpg "Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)")
Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)
Western blot: Anti-B2M antibody [EP2978Y] (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853) staining at 1/5000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 was shown to bind specifically to B2M. A band was observed at 13 kDa in wild-type A549 cell lysates with no signal observed at this size in B2M knockout cell line. To generate this image, wild-type and B2M knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853) at 1/5000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: B2M knockout A549 cell lysate at 20 µg
Lane 3: Wild-type HEK-293T ab255553 cell lysate at 20 µg
Lane 4: B2M knockout HEK-293T Human B2M (beta 2 Microglobulin) knockout HEK-293T cell line ab266828 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
![Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--flow-cytometry-intracellular-img421243.jpg "Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)")
Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853).
Flow cytometry overlay histogram showing C57 BL/6 mouse splenocytes stained with Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 (red line). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. The cells were incubated in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853) (1x 106 in 100μl at 0.2 μg/ml (1/11300)) for 30min on ice.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice
Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
![Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--western-blot-img432517.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)")
![Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769), expandable thumbnail](https://content.abcam.com/products/images/beta-2-microglobulin-antibody-ep2978y-bsa-and-azide-free-ab214769--flow-cytometry-intracellular-img421243.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (ab214769)")
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