Anti-beta 3 Adrenergic Receptor antibody (ab94506) is a rabbit polyclonal antibody that is used to detect beta 3 Adrenergic Receptor in Western Blot, IHC-P. Suitable for Mouse, Rat samples.
- Over 20 publications
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- Cancer gene therapy 30:890-9042023β3-adrenergic receptor on tumor-infiltrating lymphocytes sustains IFN-γ-dependent PD-L1 expression and impairs anti-tumor immunity in neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGennaro Bruno,Nicoletta Nastasi,Angela Subbiani,Alessia Boaretto,Sara Ciullini Mannurita,Gianluca Mattei,Patrizia Nardini,Chiara Della Bella,Alberto Magi,Alessandro Pini,Emanuela De Marco,Annalisa Tondo,Claudio Favre,Maura CalvaniPubMed 36854895
- Nature communications 13:72692022Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Duregotti,Christina M Reumiller,Ursula Mayr,Maria Hasman,Lukas E Schmidt,Sean A Burnap,Konstantinos Theofilatos,Javier Barallobre-Barreiro,Arne Beran,Maria Grandoch,Alessandro Viviano,Marjan Jahangiri,Manuel MayrPubMed 36433953
- International journal of molecular sciences 23:2022Chronic Intermittent Hypoxia Exposure Alternative to Exercise Alleviates High-Fat-Diet-Induced Obesity and Fatty Liver.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunfei Luo,Qiongfeng Chen,Junrong Zou,Jingjing Fan,Yuanjun Li,Zhijun LuoPubMed 35563600
- Clinical and translational medicine 12:e6932022The microRNA-204-5p inhibits APJ signalling and confers resistance to cardiac hypertrophy and dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRavinder Reddy Gaddam,Young-Rae Kim,Julia S Jacobs,Jin-Young Yoon,Qiuxia Li,Angela Cai,Hamsitha Shankaiahgari,Barry London,Kaikobad Irani,Ajit VikramPubMed 35060347
- The Journal of clinical investigation 132:2021β3-Adrenergic receptor downregulation leads to adipocyte catecholamine resistance in obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph M Valentine,Maryam Ahmadian,Omer Keinan,Mohammad Abu-Odeh,Peng Zhao,Xin Zhou,Mark P Keller,Hui Gao,Ruth T Yu,Christopher Liddle,Michael Downes,Jin Zhang,Aldons J Lusis,Alan D Attie,Ronald M Evans,Mikael Rydén,Alan R SaltielPubMed 34847077
- Frontiers in pharmacology 12:6979122021β2-and β3-Adrenergic Receptors Contribute to Cancer-Evoked Pain in a Mouse Model of Osteosarcoma Modulation of Neural Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGennaro Bruno,Francesco De Logu,Daniel Souza Monteiro de Araujo,Angela Subbiani,Federica Lunardi,Sofia Rettori,Romina Nassini,Claudio Favre,Maura CalvaniPubMed 34646131
- The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 25:355-3632021Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye-Jin Lee,Bo-Yeong Jin,Mi-Rae Park,Kwan Sik Seo,Yong Taek Jeong,Sang-Hyun Choi,Dong-Hoon KimPubMed 34187952
- Endocrinology 162:2021Loss of CREB Coactivator CRTC1 in SF1 Cells Leads to Hyperphagia and Obesity by High-fat Diet But Not Normal Chow Diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShigenobu Matsumura,Fuka Ishikawa,Tsutomu Sasaki,Mike Krogh Terkelsen,Kim Ravnskjaer,Tomoki Jinno,Jin Tanaka,Tsuyoshi Goto,Kazuo InouePubMed 33846709
- Cell reports 34:1088932021FoxO1 suppresses Fgf21 during hepatic insulin resistance to impair peripheral glucose utilization and acute cold tolerance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOliver Stöhr,Rongya Tao,Ji Miao,Kyle D Copps,Morris F WhitePubMed 33761350
- Cell & bioscience 11:312021The crosstalk of HDAC3, microRNA-18a and ADRB3 in the progression of heart failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingtao Na et. al.PubMed 33549119
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1-10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
Alternative names
Adrb3r, B3bar, Adrb3, Beta-3 adrenergic receptor, Beta-3 adrenoreceptor, Beta-3 adrenoceptor
Recommended products
Anti-beta 3 Adrenergic Receptor antibody (ab94506) is a rabbit polyclonal antibody that is used to detect beta 3 Adrenergic Receptor in Western Blot, IHC-P. Suitable for Mouse, Rat samples.
- Over 20 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-beta 3 Adrenergic Receptor antibody (ab94506) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), in Mouse, Rat samples.
What is the molecular weight of beta 3 Adrenergic Receptor?
Anti-beta 3 Adrenergic Receptor (ab94506) specifically detects a band for beta 3 Adrenergic Receptor (UniProt: P13945) at a molecular weight of 43kDa.
Recommended positive controls
WB: Mouse brown adipose tissue lysate.IHC-P: Mouse pancreas tissue. Mouse Adipose, Mouse Bladder, Mouse ovary.
Trusted by the scientific community
Anti-beta 3 Adrenergic Receptor (ab94506) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The beta 3 adrenergic receptor also known as beta-3 adrenoceptor or B3 adrenergic receptor is a member of the adrenergic receptor family that activates the GS protein upon binding ligands like catecholamines. This receptor approximately 408 amino acids in size has a predominant mass of around 45 kDa. It is mainly expressed in adipose tissue but also found in the gallbladder urinary bladder and the gastrointestinal tract. These receptors primarily mediate lipolysis and thermogenesis in brown adipose tissue through interaction with norepinephrine and epinephrine.
- Biological function summary
The beta 3 adrenergic receptor plays an important role in energy expenditure and metabolic processes. It does not typically form part of a complex but interacts with GS proteins leading to the activation of adenylyl cyclase and consequent increase in cAMP levels. This receptor is instrumental in the regulation of lipid metabolism which aids in maintaining balance in energy store.
- Pathways
The beta 3 adrenergic receptor is involved in the adrenergic signaling pathways regulating energy expenditure and thermogenesis. These pathways often work together with other adrenergic receptors such as beta 1 and beta 2 receptors. An important pathway involving the beta 3 adrenergic receptor is the cAMP-dependent pathway which influences metabolic functions and energy homeostasis in the body.
- Associated diseases and disorders
The beta 3 adrenergic receptor is associated with obesity and diabetes. Altered function or expression of this receptor can impact the body's ability to regulate energy metabolism leading to these metabolic disorders. The receptor's function has connections with insulin-like growth factor 1 (IGF1) as changes in energy metabolism affect insulin signaling and glucose homeostasis. Abnormal adrenergic receptor activity can also contribute to bladder disorders due to its expression in the urinary system.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab94506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506) at 1 µg/mL
Lane 1: Brown Adipose (Mouse) Tissue Lysate at 10 µg
Lane 2: Brown Adipose (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 8min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
IHC image of beta 3 Adrenergic Receptor staining in Mouse adipose (white) tissue formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94506, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
IHC image of beta 3 Adrenergic Receptor staining in Mouse Ovary Normal tissue formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94506, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
IHC image of beta 3 Adrenergic Receptor staining in Mouse Bladder Normal tissue formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94506, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab94506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506) at 1 µg/mL
All lanes: Brown Adipose (Mouse) Tissue Lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 22 kDa, 44 kDa
Exposure time: 2min
Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab94506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab94506) at 1 µg/mL
Lane 1: Mouse ovary tissue lysate at 25 µg
Lane 2: Mouse bladder tissue lysate at 25 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Exposure time: 20min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 3 Adrenergic Receptor antibody (ab94506)
IHC image of beta 3 Adrenergic Receptor staining in Mouse pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94506, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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