Name: Anti-beta Actin antibody [8F10-G10]
SKU: ab170325
Rating: 3 (3 reviews)

Mouse Monoclonal beta Actin antibody. Suitable for WB and reacts with Chinese hamster, Saccharomyces cerevisiae, African green monkey, Drosophila melanogaster, Zebrafish, Human, Goat, Rabbit, Mouse, Rat samples. Cited in 22 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTB.

Images

Western blot - Anti-beta Actin antibody [8F10-G10] (AB170325), expandable thumbnail

Publications

Key facts

Isotype: IgG2b
Host species: Mouse
Storage buffer: pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine)
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Fragment Protein within Human ACTB. The exact immunogen used to generate this antibody is proprietary information. Database link P60709

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB
Human	Tested
Mouse	Tested
Rat	Tested
African green monkey	Tested
Chinese hamster	Tested
Drosophila melanogaster	Tested
Goat	Tested
Rabbit	Tested
Saccharomyces cerevisiae	Tested
Zebrafish	Tested

Tested
Tested

Species	Dilution info	Notes
Species Chinese hamster	Dilution info 1/5000	Notes -
Species Saccharomyces cerevisiae	Dilution info 1/5000	Notes -
Species African green monkey	Dilution info 1/5000	Notes -
Species Drosophila melanogaster	Dilution info 1/5000	Notes -
Species Zebrafish	Dilution info 1/5000	Notes -
Species Human	Dilution info 1/5000	Notes -
Species Goat	Dilution info 1/5000	Notes -
Species Rabbit	Dilution info 1/5000	Notes -
Species Mouse	Dilution info 1/5000	Notes -
Species Rat	Dilution info 1/5000	Notes -

Associated Products

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Target data

See full target information ACTB

Function

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

Alternative names

Beta-actin, ACTB

Key facts

Isotype: IgG2b
Host species: Mouse
Storage buffer: pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine)
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Fragment Protein within Human ACTB. The exact immunogen used to generate this antibody is proprietary information. Database link P60709
Clone number: 8F10-G10
Purification technique: Cross-flow filtration
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Associated diseases and disorders

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

Product promise

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9 product images

Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
All lanes: Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1: COS7 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: C6 cell lysate
Lane 4: 3T3 cell lysate
Predicted band size: 41 kDa
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
This image was produced with antibody produced in ascites.
All lanes: Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/10000 dilution
Lane 1: Goat muscle cell lysate
Lane 2: CHO-K1 cell lysate
Lane 3: COS7 cell lysate
Lane 4: C6 cell lysate
Lane 5: 3T3 cell lysate
Lane 6: K562 cell lysate
Predicted band size: 41 kDa
Observed band size: 45 kDa
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
This image was produced with antibody produced in ascites.
All lanes: Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1: Rabbit spleen cell lysate
Lane 2: Rabbit colon cell lysate
Lane 3: Rabbit skeletal muscle cell lysate
Lane 4: Rabbit liver cell lysate
Lane 5: Rabbit heart cell lysate
Predicted band size: 41 kDa
Observed band size: 45 kDa
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
This image was produced with antibody produced in ascites.
Lane 1: Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
Lane 2: Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution
All lanes: Zebrafish row cell lysate
Predicted band size: 41 kDa
Observed band size: 45 kDa
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
This image was produced with antibody produced in ascites.
All lanes: Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution
Lane 1: Arabidopsis cell lysate at 30 µg
Lane 2: Arabidopsis cell lysate at 15 µg
Predicted band size: 41 kDa
Observed band size: 45 kDa
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
All lanes: Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1: S. cerevisiae cell lysate at 10 µg
Lane 2: P. pastoris cell lysate at 10 µg
Lane 3: S. cerevisiae cell lysate at 5 µg
Lane 4: P. pastoris cell lysate at 5 µg
Predicted band size: 41 kDa
Observed band size: 45 kDa
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
All lanes: Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1: COS7 cell lysate
Lane 2: 3T3 cell lysate
Lane 3: k562 cell lysate
Lane 4: HeLa cell lysate
Predicted band size: 41 kDa
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
All lanes: Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
Lane 1: Drosophila melanogaster cell lysate at 40 µg
Lane 2: Drosophila melanogaster cell lysate at 20 µg
Predicted band size: 41 kDa
Observed band size: 45 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-beta Actin antibody [8F10-G10] (ab170325)
beta Actin western blot using anti-beta Actin antibody [8F10-G10] ab170325. Publication image and figure legend from Cardoso da Silva, R., Villar-Fernández, M. A., et al., 2020, PLoS Genet, PubMed 32569318.

ab170325 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab170325 please see the product overview.
Functional analysis of the transcriptional-associated Pch2 chromosomal population.A. Representative images of immunofluorescence of Hop1 on meiotic chromosome spreads in rpo21-FRB anchor away cells, treated with DMSO or rapamycin, using the regimen indicated in Fig 3C. Chromosome synapsis was assessed by α-Gmc2 staining. B. Quantification of total Hop1 intensity per spread nucleus for the experiment shown in A and S9D Fig. n.s. (non-significant) indicates p>0.05, Mann-Whitney U test C. Schematic of treatment regimens used for anchor away experiment, as shown in D. D. Western blot analysis of Hop1 and Pch2 (α-HA) in rpo21-FRB anchor away ndt80Δ cells synchronously progressing through meiotic prophase, treated with DMSO or rapamycin, as indicated above the western blot image. Upon DMSO or rapamycin treatment samples were taken every 30 minutes. Arrow indicates phosphorylated Hop1, * indicates non-phosphorylated Hop1. See quantification of three independent western blot experiments on S4 Table.