Anti-beta Actin antibody [8F10-G10] - Loading Control

• WB

Supplier Data

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (AB170325)

This image was produced with antibody produced in ascites.

All lanes:

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (ab170325) at 1/10000 dilution

Lane 1:

Goat muscle cell lysate

Lane 2:

CHO-K1 cell lysate

Lane 3:

COS7 cell lysate

Lane 4:

C6 cell lysate

Lane 5:

3T3 cell lysate

Lane 6:

K562 cell lysate

Predicted band size: 41 kDa

Observed band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (AB170325)

All lanes:

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (ab170325) at 1/5000 dilution

Lane 1:

COS7 cell lysate

Lane 2:

3T3 cell lysate

Lane 3:

k562 cell lysate

Lane 4:

HeLa cell lysate

Predicted band size: 41 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (AB170325)

All lanes:

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (ab170325) at 1/5000 dilution

Lane 1:

COS7 cell lysate

Lane 2:

Jurkat cell lysate

Lane 3:

C6 cell lysate

Lane 4:

3T3 cell lysate

Predicted band size: 41 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (AB170325)

All lanes:

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (ab170325) at 1/3000 dilution

Lane 1:

Drosophila melanogaster cell lysate at 40 µg

Lane 2:

Drosophila melanogaster cell lysate at 20 µg

Predicted band size: 41 kDa

Observed band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (AB170325)

All lanes:

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (ab170325) at 1/5000 dilution

Lane 1:

S. cerevisiae cell lysate at 10 µg

Lane 2:

P. pastoris cell lysate at 10 µg

Lane 3:

S. cerevisiae cell lysate at 5 µg

Lane 4:

P. pastoris cell lysate at 5 µg

Predicted band size: 41 kDa

Observed band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (AB170325)

This image was produced with antibody produced in ascites.

All lanes:

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (ab170325) at 1/5000 dilution

Lane 1:

Rabbit spleen cell lysate

Lane 2:

Rabbit colon cell lysate

Lane 3:

Rabbit skeletal muscle cell lysate

Lane 4:

Rabbit liver cell lysate

Lane 5:

Rabbit heart cell lysate

Predicted band size: 41 kDa

Observed band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (AB170325)

This image was produced with antibody produced in ascites.

Lane 1:

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (ab170325) at 1/3000 dilution

Lane 2:

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (ab170325) at 1/1000 dilution

All lanes:

Zebrafish row cell lysate

Predicted band size: 41 kDa

Observed band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (AB170325)

This image was produced with antibody produced in ascites.

All lanes:

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (ab170325) at 1/1000 dilution

Lane 1:

Arabidopsis cell lysate at 30 µg

Lane 2:

Arabidopsis cell lysate at 15 µg

Predicted band size: 41 kDa

Observed band size: 45 kDa

false

• WB

CiteAb

Western blot - Anti-beta Actin antibody [8F10-G10] - Loading Control (AB170325)

beta Actin western blot using anti-beta Actin antibody [8F10-G10] ab170325. Publication image and figure legend from Cardoso da Silva, R., Villar-Fernández, M. A., et al., 2020, PLoS Genet, PubMed 32569318.

ab170325 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab170325 please see the product overview.

Functional analysis of the transcriptional-associated Pch2 chromosomal population.A. Representative images of immunofluorescence of Hop1 on meiotic chromosome spreads in rpo21-FRB anchor away cells, treated with DMSO or rapamycin, using the regimen indicated in Fig 3C. Chromosome synapsis was assessed by α-Gmc2 staining. B. Quantification of total Hop1 intensity per spread nucleus for the experiment shown in A and S9D Fig. n.s. (non-significant) indicates p>0.05, Mann-Whitney U test C. Schematic of treatment regimens used for anchor away experiment, as shown in D. D. Western blot analysis of Hop1 and Pch2 (α-HA) in rpo21-FRB anchor away ndt80Δ cells synchronously progressing through meiotic prophase, treated with DMSO or rapamycin, as indicated above the western blot image. Upon DMSO or rapamycin treatment samples were taken every 30 minutes. Arrow indicates phosphorylated Hop1, * indicates non-phosphorylated Hop1. See quantification of three independent western blot experiments on S4 Table.

false