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Rabbit Polyclonal beta Actin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, Chicken, Chinese hamster, Cow, Dog, Fish, Rabbit, Xenopus laevis samples. Cited in 4034 publications.
Alternative names=Beta-actin, ACTB
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Tested |
| Rat | Expected | Tested | Tested |
| Chicken | Expected | Expected | Expected |
| Chinese hamster | Expected | Expected | Expected |
| Cow | Expected | Expected | Expected |
| Dog | Expected | Expected | Expected |
| Drosophila melanogaster | Predicted | Predicted | Predicted |
| Fish | Expected | Expected | Expected |
| Guinea pig | Predicted | Predicted | Predicted |
| Monkey | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Expected | Expected | Expected |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
| Xenopus laevis | Expected | Expected | Expected |
| Zebrafish | Predicted | Predicted | Predicted |
Images
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.20000-1.00000 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Biotin) (ab6720) secondary antibody or Goat Anti-Rabbit IgG Fc (HRP) (ab97200) secondary antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info 0.20000-1.00000 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Biotin) (ab6720) secondary antibody or Goat Anti-Rabbit IgG Fc (HRP) (ab97200) secondary antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Chicken | Dilution info 0.2-1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Biotin) (ab6720) secondary antibod or Goat Anti-Rabbit IgG Fc (HRP) (ab97200) secondary antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Chinese hamster | Dilution info 0.2-1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Biotin) (ab6720) secondary antibod or Goat Anti-Rabbit IgG Fc (HRP) (ab97200) secondary antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Cow | Dilution info 0.2-1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Biotin) (ab6720) secondary antibod or Goat Anti-Rabbit IgG Fc (HRP) (ab97200) secondary antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Dog | Dilution info 0.2-1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Biotin) (ab6720) secondary antibod or Goat Anti-Rabbit IgG Fc (HRP) (ab97200) secondary antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Fish | Dilution info 0.2-1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Biotin) (ab6720) secondary antibod or Goat Anti-Rabbit IgG Fc (HRP) (ab97200) secondary antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rabbit | Dilution info 0.2-1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Biotin) (ab6720) secondary antibod or Goat Anti-Rabbit IgG Fc (HRP) (ab97200) secondary antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Xenopus laevis | Dilution info 0.2-1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Biotin) (ab6720) secondary antibod or Goat Anti-Rabbit IgG Fc (HRP) (ab97200) secondary antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig, Drosophila melanogaster, Monkey, Zebrafish, Rhesus monkey, Sheep | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems. |
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems. |
Species Rat | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems. |
Species Fish | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems. |
Species Xenopus laevis | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems. |
Species Dog | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems. |
Species Cow | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems. |
Species Chicken | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems. |
Species Rabbit | Dilution info 1/1000.00000 - 1/5000.00000 | Notes We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody. A stronger signal is observed in chemiluminescent western blot when using 2% BSA as the blocking agent. Milk blocking is suitable for use with fluorescent detection systems. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig, Drosophila melanogaster, Monkey, Zebrafish, Rhesus monkey, Sheep | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken | Dilution info 1 µg/mL | Notes - |
Species Chinese hamster | Dilution info 1 µg/mL | Notes - |
Species Cow | Dilution info 1 µg/mL | Notes - |
Species Dog | Dilution info 1 µg/mL | Notes - |
Species Fish | Dilution info 1 µg/mL | Notes - |
Species Rabbit | Dilution info 1 µg/mL | Notes - |
Species Xenopus laevis | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig, Drosophila melanogaster, Monkey, Zebrafish, Rhesus monkey, Sheep | Dilution info - | Notes - |
Recommended products
Rabbit Polyclonal beta Actin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, Chicken, Chinese hamster, Cow, Dog, Fish, Rabbit, Xenopus laevis samples. Cited in 4034 publications.
Alternative names=Beta-actin, ACTB
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. Cross-reactivity with this protein has not been confirmed experimentally.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
For western blot, milk blocking is suitable for use with fluorescent detection systems. For western blot using chemiluminescent (ECL) systems we recommend BSA blocking.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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